The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.
PCR primers to amplify 16S rRNA genes from cyanobacteria
Open accessAbstract
We developed and tested a set of oligonucleotide primers for the specific amplification of 16S rRNA gene segments from cyanobacteria and plastids by PCR. PCR products were recovered from all cultures of cyanobacteria and diatoms that were checked but not from other bacteria and archaea. Gene segments selectively retrieved from cyanobacteria and diatoms in unialgal but nonaxenic cultures and from cyanobionts in lichens could be directly sequenced. In the context of growing sequence databases, this procedure allows rapid and phylogenetically meaningful identification without pure cultures or molecular cloning. We demonstrate the use of this specific PCR in combination with denaturing gradient gel electrophoresis to probe the diversity of oxygenic phototrophic microorganisms in cultures, lichens, and complex microbial communities.
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Altschul, S. F., Gish, W., Miller, W., Myers, E. W., & Lipman, D. J. (1990) · Journal of Molecular Biology · doi:10.1006/jmbi.1990.9999 · Cited by 98,669
Generic Assignments, Strain Histories and Properties of Pure Cultures of Cyanobacteria
Rippka, R., Stanier, R. Y., Deruelles, J., Herdman, M., & Waterbury, J. B. (1979) · Microbiology · doi:10.1099/00221287-111-1-1 · Cited by 7,606
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Fuller, N. J., Marie, D., Partensky, F., Vaulot, D., Post, A. F., & Scanlan, D. J. (2003) · Applied and Environmental Microbiology · doi:10.1128/aem.69.5.2430-2443.2003 · Cited by 316
Mühling, M., Woolven-Allen, J., Murrell, J. C., & Joint, I. (2008) · The ISME Journal · doi:10.1038/ismej.2007.97 · Cited by 304
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Rashby, S. E., Sessions, A. L., Summons, R. E., & Newman, D. K. (2007) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0704912104 · Cited by 281
An early-branching microbialite cyanobacterium forms intracellular carbonates
Couradeau, E., Benzerara, K., Gérard, E., Moreira, D., Bernard, S., Brown, G. E., & López-García, P. (2012) · Science · doi:10.1126/science.1216171 · Cited by 263
Metadata
- Authors
- Nübel, U., Garcia-Pichel, F., & Muyzer, G.
- Venue
- Applied and Environmental Microbiology
- Publisher
- American Society for Microbiology
- Published
- 1997-08
- Volume
- 63
- Issue
- 8
- Pages
- 3327-3332
- ISSN
- 0099-2240 (ISSN-L)
- 1098-5336
- OpenAlex
- W2159620478
- Cited by
- 1,513
- References
- 2
- FWCI
- 14.50
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Nübel, U., Garcia-Pichel, F., & Muyzer, G. (1997). PCR primers to amplify 16S rRNA genes from cyanobacteria. Applied and Environmental Microbiology. https://doi.org/10.1128/aem.63.8.3327-3332.1997
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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