The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
New Unstable Variants of Green Fluorescent Protein for Studies of Transient Gene Expression in Bacteria
Open accessAbstract
Use of the green fluorescent protein (Gfp) from the jellyfish Aequorea victoria is a powerful method for nondestructive in situ monitoring, since expression of green fluorescence does not require any substrate addition. To expand the use of Gfp as a reporter protein, new variants have been constructed by the addition of short peptide sequences to the C-terminal end of intact Gfp. This rendered the Gfp susceptible to the action of indigenous housekeeping proteases, resulting in protein variants with half-lives ranging from 40 min to a few hours when synthesized in Escherichia coli and Pseudomonas putida . The new Gfp variants should be useful for in situ studies of temporal gene expression.
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Cites
Role of a peptide tagging system in degradation of proteins synthesized from damaged messenger RNA
Keiler, K. C., Waller, P. R. H., & Sauer, R. T. (1996) · Science · doi:10.1126/science.271.5251.990 · Cited by 1,125
Cited by
Stochastic Gene Expression in a Single Cell
Elowitz, M. B., Levine, A. J., Siggia, E. D., & Swain, P. S. (2002) · Science · doi:10.1126/science.1070919 · Cited by 5,441
Wilks, J. C., & Slonczewski, J. L. (2007) · Journal of Bacteriology · doi:10.1128/jb.00615-07 · Cited by 366
Next-generation synthetic gene networks
Lu, T. K., Khalil, A. S., & Collins, J. J. (2009) · Nature Biotechnology · doi:10.1038/nbt.1591 · Cited by 365
El Meouche, I., Siu, Y., & Dunlop, M. J. (2016) · Scientific Reports · doi:10.1038/srep19538 · Cited by 120
Detje, C. N., Lienenklaus, S., Chhatbar, C., Spanier, J., Prajeeth, C. K., Soldner, C., Tovey, M. G., Schlüter, D., Weiss, S., Stangel, M., et al. (2015) · Journal of Virology · doi:10.1128/jvi.02044-14 · Cited by 76
Metadata
- Authors
- Andersen, J. B., Sternberg, C., Poulsen, L. K., Bjørn, S. P., Givskov, M., & Molin, S.
- Venue
- Applied and Environmental Microbiology
- Publisher
- American Society for Microbiology
- Published
- 1998-06
- Volume
- 64
- Issue
- 6
- Pages
- 2240-2246
- ISSN
- 0099-2240 (ISSN-L)
- 1098-5336
- OpenAlex
- W1540505601
- Cited by
- 1,033
- References
- 1
- FWCI
- 22.45
- Type
- journal article
- Language
- en
- Open access
- green
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- The paper
- Andersen, J. B., Sternberg, C., Poulsen, L. K., Bjørn, S. P., Givskov, M., & Molin, S. (1998). New Unstable Variants of Green Fluorescent Protein for Studies of Transient Gene Expression in Bacteria. Applied and Environmental Microbiology. https://doi.org/10.1128/aem.64.6.2240-2246.1998
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
