The DOI matches its Crossref record.
Enumeration of Marine Viruses in Culture and Natural Samples by Flow Cytometry
Open accessAbstract
Flow cytometry (FCM) was successfully used to enumerate viruses in seawater after staining with the nucleic acid-specific dye SYBR Green-I. The technique was first optimized by using the Phaeocystis lytic virus PpV-01. Then it was used to analyze natural samples from different oceanic locations. Virus samples were fixed with 0.5% glutaraldehyde and deep frozen for delayed analysis. The samples were then diluted in Tris-EDTA buffer and analyzed in the presence of SYBR Green-I. A duplicate sample was heated at 80°C in the presence of detergent before analysis. Virus counts obtained by FCM were highly correlated to, although slightly higher than, those obtained by epifluorescence microscopy or by transmission electron microscopy ( r = 0.937, n = 14, and r = 0.96, n = 8, respectively). Analysis of a depth profile from the Mediterranean Sea revealed that the abundance of viruses displayed the same vertical trend as that of planktonic cells. FCM permits us to distinguish between at least two and sometimes three virus populations in natural samples. Because of its speed and accuracy, FCM should prove very useful for studies of virus infection in cultures and should allow us to better understand the structure and dynamics of virus populations in natural waters.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cited by
Interactions between microplastics and phytoplankton aggregates: Impact on their respective fates
Long, M., Moriceau, B., Gallinari, M., Lambert, C., Huvet, A., Raffray, J., & Soudant, P. (2015) · Marine Chemistry · doi:10.1016/j.marchem.2015.04.003 · Cited by 711
Viral Control of Phytoplankton Populations—a Review1
BRUSSAARD, C. P. D. (2004) · Journal of Eukaryotic Microbiology · doi:10.1111/j.1550-7408.2004.tb00537.x · Cited by 501
Self-citationYoch, D. C. (2002) · Applied and Environmental Microbiology · doi:10.1128/aem.68.12.5804-5815.2002 · Cited by 452
Past, present and future applications of flow cytometry in aquatic microbiology
Wang, Y., Hammes, F., De Roy, K., Verstraete, W., & Boon, N. (2010) · Trends in Biotechnology · doi:10.1016/j.tibtech.2010.04.006 · Cited by 247
Flow cytometry and the single cell in phycology
Collier, J. L. (2000) · Journal of Phycology · doi:10.1046/j.1529-8817.2000.99215.x · Cited by 136
Stable coexistence in marine algal host-virus systems
Thyrhaug, R., Larsen, A., Thingstad, T., & Bratbak, G. (2003) · Marine Ecology Progress Series · doi:10.3354/meps254027 · Cited by 128
Self-citationWang, Y., Liu, Y., Wang, J., Luo, T., Zhang, R., Sun, J., Zheng, Q., & Jiao, N. (2019) · Marine Pollution Bulletin · doi:10.1016/j.marpolbul.2019.03.035 · Cited by 45
Metadata
- Authors
- Marie, D., Brussaard, C. P. D., Thyrhaug, R., Bratbak, G., & Vaulot, D.
- Venue
- Applied and Environmental Microbiology
- Publisher
- American Society for Microbiology
- Published
- 1999-01
- Volume
- 65
- Issue
- 1
- Pages
- 45-52
- ISSN
- 0099-2240 (ISSN-L)
- 1098-5336
- OpenAlex
- W2102820270
- Cited by
- 646
- References
- 0
- FWCI
- 20.30
- Type
- journal article
- Language
- en
- Open access
- green
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
