Cited by 67Vibrio bacteria research studiesAquaculture disease management and microbiotaEnterobacteriaceae and Cronobacter Research

Arbitrarily Primed PCR To Type Vibrio spp. Pathogenic for Shrimp

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Goarant, Cyrille, Merien, Fabrice, Berthe, Franck, Mermoud, Isabelle, Perolat, Philippe·1999·Applied and Environmental Microbiology

Abstract

A molecular typing study on Vibrio strains implicated in shrimp disease outbreaks in New Caledonia and Japan was conducted by using AP-PCR (arbitrarily primed PCR). It allowed rapid identification of isolates at the genospecies level and studies of infraspecific population structures of epidemiological interest. Clusters identified within the species Vibrio penaeicida were related to their area of origin, allowing discrimination between Japanese and New Caledonian isolates, as well as between those from two different bays in New Caledonia separated by only 50 km. Other subclusters of New Caledonian V. penaeicida isolates could be identified, but it was not possible to link those differences to accurate epidemiological features. This contribution of AP-PCR to the study of vibriosis in penaeid shrimps demonstrates its high discriminating power and the relevance of the epidemiological information provided. This approach would contribute to better knowledge of the ecology of Vibrio spp. and their implication in shrimp disease in aquaculture.

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Crossref · 10.1128/aem.65.3.1145-1151.1999

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Authors
Goarant, C., Merien, F., Berthe, F., Mermoud, I., & Perolat, P.
Affiliations
  • Ifremer (FR)
  • <!--label omitted: 2-->Laboratoire des Leptospires, Institut Pasteur de Nouvelle-Calédonie,2 and
  • Centre de Coopération Internationale en Recherche Agronomique pour le Développement (FR)
Venue
Applied and Environmental Microbiology
Published
1999-03
Volume
65
Issue
3
Pages
1145-1151
ISSN
  • 0099-2240 (ISSN-L)
  • 1098-5336
OpenAlex
W2140687394
Cited by
67
References
31
Citations by year
2023: 1 · 2022: 1 · 2021: 6 · 2020: 1 · 2019: 1 · 2018: 2 · 2016: 2 · 2015: 1 · 2014: 6 · 2013: 3 · 2012: 1
FWCI
0.83
Type
journal article
Language
en
Open access
Locations
  • Applied and Environmental Microbiology (publishedVersion)
  • Europe PMC (PubMed Central) (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
  • HAL (submittedVersion, other-oa)
  • Institutional Archive of Ifremer (French Research Institute for Exploitation of the Sea) (submittedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Endocrinology › Vibrio bacteria research studies
  • Life Sciences › Immunology and Microbiology › Immunology › Aquaculture disease management and microbiota
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Endocrinology › Enterobacteriaceae and Cronobacter Research
Keywords
Vibrio penaeicida, Vibrio spp., shrimp disease, vibriosis, penaeid shrimp, AP-PCR, population structure, New Caledonia, Japan, aquaculture
MeSH terms
Animals, Disease Outbreaks, New Caledonia, Vibrio, Bacterial Typing Techniques, Polymerase Chain Reaction, DNA Fingerprinting, Aquaculture, Decapoda

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