Arbitrarily Primed PCR To Type Vibrio spp. Pathogenic for Shrimp
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A molecular typing study on Vibrio strains implicated in shrimp disease outbreaks in New Caledonia and Japan was conducted by using AP-PCR (arbitrarily primed PCR). It allowed rapid identification of isolates at the genospecies level and studies of infraspecific population structures of epidemiological interest. Clusters identified within the species Vibrio penaeicida were related to their area of origin, allowing discrimination between Japanese and New Caledonian isolates, as well as between those from two different bays in New Caledonia separated by only 50 km. Other subclusters of New Caledonian V. penaeicida isolates could be identified, but it was not possible to link those differences to accurate epidemiological features. This contribution of AP-PCR to the study of vibriosis in penaeid shrimps demonstrates its high discriminating power and the relevance of the epidemiological information provided. This approach would contribute to better knowledge of the ecology of Vibrio spp. and their implication in shrimp disease in aquaculture.
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Production of Litopenaeus stylirostris in New Caledonia has been declining due to seasonal vibriosis touching adult individuals and to larval mortalities that have not yet been explained.
“Unfortunately, the production has been declining due to seasonal vibriosis touching adult individuals (Goarant et al., 1999; Goarant et al., 2006) and to larval mortalities that have not yet been explained.”
From Potential lineage transmission within the active microbiota of the eggs and the nauplii of the shrimp <i>Litopenaeus stylirostris</i> : possible influence of the rearing water and more (2021) · cites this paper as Goarant et al., 1999
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- Authors
- Goarant, C., Merien, F., Berthe, F., Mermoud, I., & Perolat, P.
- Affiliations
- Ifremer (FR)
- <!--label omitted: 2-->Laboratoire des Leptospires, Institut Pasteur de Nouvelle-Calédonie,2 and
- Centre de Coopération Internationale en Recherche Agronomique pour le Développement (FR)
- Venue
- Applied and Environmental Microbiology
- Publisher
- American Society for Microbiology
- Published
- 1999-03
- Volume
- 65
- Issue
- 3
- Pages
- 1145-1151
- ISSN
- 0099-2240 (ISSN-L)
- 1098-5336
- PMC
- PMC91156
- OpenAlex
- W2140687394
- Cited by
- 67
- References
- 31
- Citations by year
- 2023: 1 · 2022: 1 · 2021: 6 · 2020: 1 · 2019: 1 · 2018: 2 · 2016: 2 · 2015: 1 · 2014: 6 · 2013: 3 · 2012: 1
- FWCI
- 0.83
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Applied and Environmental Microbiology (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- HAL (submittedVersion, other-oa)
- Institutional Archive of Ifremer (French Research Institute for Exploitation of the Sea) (submittedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Endocrinology › Vibrio bacteria research studies
- Life Sciences › Immunology and Microbiology › Immunology › Aquaculture disease management and microbiota
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Endocrinology › Enterobacteriaceae and Cronobacter Research
- Keywords
- Vibrio penaeicida, Vibrio spp., shrimp disease, vibriosis, penaeid shrimp, AP-PCR, population structure, New Caledonia, Japan, aquaculture
- MeSH terms
- Animals, Disease Outbreaks, New Caledonia, Vibrio, Bacterial Typing Techniques, Polymerase Chain Reaction, DNA Fingerprinting, Aquaculture, Decapoda
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