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Bacterioplankton Compositions of Lakes and Oceans: a First Comparison Based on Fluorescence In Situ Hybridization

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Glöckner, Frank Oliver, Fuchs, Bernhard M., Amann, Rudolf·1999·Applied and Environmental Microbiology

Abstract

Fluorescence in situ hybridization (FISH) with rRNA-targeted oligonucleotide probes was used to investigate the phylogenetic composition of bacterioplankton communities in several freshwater and marine samples. An average of about 50% of the cells were detected by probes for the domains Bacteria and Archaea , and of these, about half could be identified at the subdomain level with a set of group-specific probes. Beta subclass proteobacteria constituted a dominant fraction in freshwater systems, accounting for 16% (range, 3 to 32%) of the cells, although they were essentially absent in the marine samples examined. Members of the Cytophaga-Flavobacterium cluster were the most abundant group detected in the marine systems, accounting for 18% (range, 2 to 72%) of the 4′,6-diamidino-2-phenylindole (DAPI) counts, and they were also important in freshwater systems (7%, range 0 to 18%). Furthermore, members of the alpha and gamma subclasses of Proteobacteria as well as members of the Planctomycetales were detected in both freshwater and marine water in abundances <7%.

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Crossref · 10.1128/aem.65.8.3721-3726.1999

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Metadata

Authors
Glöckner, F. O., Fuchs, B. M., & Amann, R.
Venue
Applied and Environmental Microbiology
Published
1999-08
Volume
65
Issue
8
Pages
3721-3726
ISSN
  • 0099-2240 (ISSN-L)
  • 1098-5336
OpenAlex
W1527271978
Cited by
816
References
1
FWCI
37.29
Type
journal article
Language
en
Open access
green

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CoChat. (2026). Citation check: Bacterioplankton Compositions of Lakes and Oceans: a First Comparison Based on Fluorescence In Situ Hybridization. https://app.cochat.ai/papers/10.1128/aem.65.8.3721-3726.1999
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Glöckner, F. O., Fuchs, B. M., & Amann, R. (1999). Bacterioplankton Compositions of Lakes and Oceans: a First Comparison Based on Fluorescence In Situ Hybridization. Applied and Environmental Microbiology. https://doi.org/10.1128/aem.65.8.3721-3726.1999

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