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A PCR Assay To Discriminate Human and Ruminant Feces on the Basis of Host Differences in Bacteroides-Prevotella Genes Encoding 16S rRNA
Open accessAbstract
Our purpose was to develop a rapid, inexpensive method of diagnosing the source of fecal pollution in water. In previous research, we identified Bacteroides-Prevotella ribosomal DNA (rDNA) PCR markers based on analysis. These markers length heterogeneity PCR and terminal restriction fragment length polymorphism distinguish cow from human feces. Here, we recovered 16S rDNA clones from natural waters that were close phylogenetic relatives of the markers. From the sequence data, we designed specific PCR primers that discriminate human and ruminant sources of fecal contamination.
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Cited by
Kildare, B. J., Leutenegger, C. M., McSwain, B. S., Bambic, D. G., Rajal, V. B., & Wuertz, S. (2007) · Water Research · doi:10.1016/j.watres.2007.06.037 · Cited by 451
Performance of forty-one microbial source tracking methods: A twenty-seven lab evaluation study
Boehm, A. B., Van De Werfhorst, L. C., Griffith, J. F., Holden, P. A., Jay, J. A., Shanks, O. C., Wang, D., & Weisberg, S. B. (2013) · Water Research · doi:10.1016/j.watres.2012.12.046 · Cited by 313
Pickering, A. J., Davis, J., Walters, S. P., Horak, H. M., Keymer, D. P., Mushi, D., Strickfaden, R., Chynoweth, J. S., Liu, J., Blum, A., et al. (2010) · Environmental Science & Technology · doi:10.1021/es903524m · Cited by 164
Liszt, K., Zwielehner, J., Handschur, M., Hippe, B., Thaler, R., & Haslberger, A. G. (2009) · Annals of Nutrition and Metabolism · doi:10.1159/000229505 · Cited by 61
Metadata
- Authors
- Bernhard, A. E., & Field, K. G.
- Venue
- Applied and Environmental Microbiology
- Publisher
- American Society for Microbiology
- Published
- 2000-10
- Volume
- 66
- Issue
- 10
- Pages
- 4571-4574
- ISSN
- 0099-2240 (ISSN-L)
- 1098-5336
- OpenAlex
- W2121409138
- Cited by
- 692
- References
- 0
- FWCI
- 16.05
- Type
- journal article
- Language
- en
- Open access
- green
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- CoChat. (2026). Citation check: A PCR Assay To Discriminate Human and Ruminant Feces on the Basis of Host Differences in Bacteroides-Prevotella Genes Encoding 16S rRNA. https://app.cochat.ai/papers/10.1128/aem.66.10.4571-4574.2000
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- Bernhard, A. E., & Field, K. G. (2000). A PCR Assay To Discriminate Human and Ruminant Feces on the Basis of Host Differences in Bacteroides-Prevotella Genes Encoding 16S rRNA. Applied and Environmental Microbiology. https://doi.org/10.1128/aem.66.10.4571-4574.2000
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
