The DOI matches its Crossref record; 31 of the 31 references it cites were found in a registry or an index.
Cellular Microcystin Content in N-Limited Microcystis aeruginosa Can Be Predicted from Growth Rate
Open accessAbstract
Cell quotas of microcystin ( Q MCYST ; femtomoles of MCYST per cell), protein, and chlorophyll a (Chl a ), cell dry weight, and cell volume were measured over a range of growth rates in N-limited chemostat cultures of the toxic cyanobacterium Microcystis aeruginosa MASH 01-A19. There was a positive linear relationship between Q MCYST and specific growth rate (μ), from which we propose a generalized model that enables Q MCYST at any nutrient-limited growth rate to be predicted based on a single batch culture experiment. The model predicts Q MCYST from μ, μ max (maximum specific growth rate), Q MCYSTmax (maximum cell quota), and Q MCYSTmin (minimum cell quota). Under the conditions examined in this study, we predict a Q MCYSTmax of 0.129 fmol cell −1 at μ max and a Q MCYSTmin of 0.050 fmol cell −1 at μ = 0. Net MCYST production rate ( R MCYST ) asymptotes to zero at μ = 0 and reaches a maximum of 0.155 fmol cell −1 day −1 at μ max . MCYST/dry weight ratio (milligrams per gram [dry weight]) increased linearly with μ, whereas the MCYST/protein ratio reached a maximum at intermediate μ. In contrast, the MCYST/Chl a ratio remained constant. Cell volume correlated negatively with μ, leading to an increase in intracellular MCYST concentration at high μ. Taken together, our results show that fast-growing cells of N-limited M. aeruginosa are smaller, are of lower mass, and have a higher intracellular MCYST quota and concentration than slow-growing cells. The data also highlight the importance of determining cell MCYST quotas, as potentially confusing interpretations can arise from determining MCYST content as a ratio to other cell components.
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Cites
PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENT
Lowry, O., Rosebrough, N., Farr, A. L., & Randall, R. (1951) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)52451-6 · Cited by 317,331
Toxic Cyanobacteria in Water: A Guide to their Public Health Consequences, Monitoring and Management
(1999) · CRC Press eBooks · doi:10.1201/9781482295061 · Cited by 2,574
MacKintosh, C., Beattie, K. A., Klumpp, S., Cohen, P., & Codd, G. A. (1990) · FEBS Letters · doi:10.1016/0014-5793(90)80245-e · Cited by 1,653
Liver Failure and Death after Exposure to Microcystins at a Hemodialysis Center in Brazil
Jochimsen, E. M., Carmichael, W. W., An, J., Cardo, D. M., Cookson, S. T., Holmes, C. E. M., Antunes, M. B., de Melo Filho, D. A., Lyra, T. M., Barreto, V. S. T., et al. (1998) · New England Journal of Medicine · doi:10.1056/nejm199803263381304 · Cited by 1,109
SOME THOUGHTS ON NUTRIENT LIMITATION IN ALGAE1
Droop, M. R. (1973) · Journal of Phycology · doi:10.1111/j.1529-8817.1973.tb04092.x · Cited by 813
Fatal microcystin intoxication in haemodialysis unit in Caruaru, Brazil
Pouria, S., de Andrade, A., Barbosa, J., Cavalcanti, R., Barreto, V., Ward, C., Preiser, W., Poon, G. K., Neild, G., & Codd, G. (1998) · The Lancet · doi:10.1016/s0140-6736(97)12285-1 · Cited by 812
Structure and biosynthesis of toxins from blue-green algae (cyanobacteria)
Rinehart, K. L., Namikoshi, M., & Choi, B. W. (1994) · Journal of Applied Phycology · doi:10.1007/bf02186070 · Cited by 515
Orr, P. T., & Jones, G. J. (1998) · Limnology and Oceanography · doi:10.4319/lo.1998.43.7.1604 · Cited by 471
Self-citationDittmann, E., Neilan, B. A., Erhard, M., Von Döhren, H., & Börner, T. (1997) · Molecular Microbiology · doi:10.1046/j.1365-2958.1997.6131982.x · Cited by 395
Arnon, D. I., McSwain, B. D., Tsujimoto, H. Y., & Wada, K. (1974) · Biochimica et Biophysica Acta (BBA) - Bioenergetics · doi:10.1016/0005-2728(74)90063-2 · Cited by 393
Cited by
The rise of harmful cyanobacteria blooms: the potential roles of eutrophication and climate change
O’Neil, J. M., Davis, T. W., Burford, M. A., & Gobler, C. J. (2012) · Harmful Algae · doi:10.1016/j.hal.2011.10.027 · Cited by 2,225
Harke, M. J., Steffen, M. M., Gobler, C. J., Otten, T. G., Wilhelm, S. W., Wood, S. A., & Paerl, H. W. (2016) · Harmful Algae · doi:10.1016/j.hal.2015.12.007 · Cited by 1,084
Microcystin-producing blooms—a serious global public health issue
de Figueiredo, D. R., Azeiteiro, U. M., Esteves, S. M., Gonçalves, F. J. M., & Pereira, M. J. (2004) · Ecotoxicology and Environmental Safety · doi:10.1016/j.ecoenv.2004.04.006 · Cited by 587
Rae, B. D., Long, B. M., Badger, M. R., & Price, G. D. (2013) · Microbiology and Molecular Biology Reviews · doi:10.1128/mmbr.00061-12 · Cited by 409
Self-citationVézie, C., Rapala, J., Vaitomaa, J., Seitsonen, J., & Sivonen, K. (2002) · Microbial Ecology · doi:10.1007/s00248-001-0041-9 · Cited by 332
Metadata
- Authors
- Long, B. M., Jones, G. J., & Orr, P. T.
- Venue
- Applied and Environmental Microbiology
- Publisher
- American Society for Microbiology
- Published
- 2001-01
- Volume
- 67
- Issue
- 1
- Pages
- 278-283
- ISSN
- 0099-2240 (ISSN-L)
- 1098-5336
- OpenAlex
- W2168154005
- Cited by
- 299
- References
- 31
- FWCI
- 5.14
- Type
- journal article
- Language
- en
- Open access
- green
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