The DOI matches its Crossref record; 26 of the 26 references it cites were found in a registry or an index.
Differentiation of Lactobacillus plantarum, L. pentosus, and L. paraplantarum by recA Gene Sequence Analysis and Multiplex PCR Assay with recA Gene-Derived Primers
Open accessAbstract
In this study, we succeeded in differentiating Lactobacillus plantarum, Lactobacillus pentosus, and Lactobacillus paraplantarum by means of recA gene sequence comparison. Short homologous regions of about 360 bp were amplified by PCR with degenerate consensus primers, sequenced, and analyzed, and 322 bp were considered for the inference of phylogenetic trees. Phylograms, obtained by parsimony, maximum likelihood, and analysis of data matrices with the neighbor-joining model, were coherent and clearly separated the three species. The validity of the recA gene and RecA protein as phylogenetic markers is discussed. Based on the same sequences, species-specific primers were designed, and a multiplex PCR protocol for the simultaneous distinction of these bacteria was optimized. The sizes of the amplicons were 318 bp for L. plantarum , 218 bp for L. pentosus, and 107 bp for L. paraplantarum . This strategy permitted the unambiguous identification of strains belonging to L. plantarum, L. pentosus, and L. paraplantarum in a single reaction, indicating its applicability to the speciation of isolates of the L. plantarum group.
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Gapped BLAST and PSI-BLAST: a new generation of protein database search programs
Altschul, S. (1997) · Nucleic Acids Research · doi:10.1093/nar/25.17.3389 · Cited by 73,302
Thompson, J. D., Higgins, D. G., & Gibson, T. J. (1994) · Nucleic Acids Research · doi:10.1093/nar/22.22.4673 · Cited by 64,617
The neighbor-joining method: a new method for reconstructing phylogenetic trees.
(1987) · Molecular Biology and Evolution · doi:10.1093/oxfordjournals.molbev.a040454 · Cited by 59,715
A procedure for the isolation of deoxyribonucleic acid from micro-organisms
Marmur, J. (1961) · Journal of Molecular Biology · doi:10.1016/s0022-2836(61)80047-8 · Cited by 11,545
Bergey's Manual of Determinative Bacteriology
S., F. A., Buchanan, R. E. T., Gibbons, N. E., & Bergey (1975) · Taxon · doi:10.2307/1218353 · Cited by 11,333
STACKEBRANDT, E., & GOEBEL, B. M. (1994) · International Journal of Systematic and Evolutionary Microbiology · doi:10.1099/00207713-44-4-846 · Cited by 7,083
How Close Is Close: 16S rRNA Sequence Identity May Not Be Sufficient To Guarantee Species Identity
Fox, G. E., Wisotzkey, J. D., & Jurtshuk, P. (1992) · International Journal of Systematic Bacteriology · doi:10.1099/00207713-42-1-166 · Cited by 1,300
Structure of the recA protein–ADP complex
Story, R. M., & Steitz, T. A. (1992) · Nature · doi:10.1038/355374a0 · Cited by 624
Collins, M. D., Rodrigues, U., Ash, C., Aguirre, M., Farrow, J. A. E., Martinez-Murcia, A., Phillips, B. A., Williams, A. M., & Wallbanks, S. (1991) · FEMS Microbiology Letters · doi:10.1111/j.1574-6968.1991.tb04313.x · Cited by 383
Palys, T., Nakamura, L. K., & Cohan, F. M. (1997) · International Journal of Systematic Bacteriology · doi:10.1099/00207713-47-4-1145 · Cited by 381
Cited by
Zheng, J., Wittouck, S., Salvetti, E., Franz, C. M. A. P., Harris, H. M. B., Mattarelli, P., O’Toole, P. W., Pot, B., Vandamme, P., Walter, J., et al. (2020) · International Journal of Systematic and Evolutionary Microbiology · doi:10.1099/ijsem.0.004107 · Cited by 3,313
Self-citationGenomic Diversity and Versatility of Lactobacillus plantarum, a Natural Metabolic Engineer
Siezen, R. J., & van Hylckama Vlieg, J. E. (2011) · Microbial Cell Factories · doi:10.1186/1475-2859-10-s1-s3 · Cited by 294
Biodiversity and identification of sourdough lactic acid bacteria
De Vuyst, L., & Vancanneyt, M. (2007) · Food Microbiology · doi:10.1016/j.fm.2006.07.005 · Cited by 232
Ennahar, S., Cai, Y., & Fujita, Y. (2003) · Applied and Environmental Microbiology · doi:10.1128/aem.69.1.444-451.2003 · Cited by 215
Identification of potential probiotic starter cultures for Scandinavian-type fermented sausages
Klingberg, T. D., Axelsson, L., Naterstad, K., Elsser, D., & Budde, B. B. (2005) · International Journal of Food Microbiology · doi:10.1016/j.ijfoodmicro.2005.03.020 · Cited by 186
Metadata
- Authors
- Torriani, S., Felis, G. E., & Dellaglio, F.
- Venue
- Applied and Environmental Microbiology
- Publisher
- American Society for Microbiology
- Published
- 2001-08
- Volume
- 67
- Issue
- 8
- Pages
- 3450-3454
- ISSN
- 0099-2240 (ISSN-L)
- 1098-5336
- OpenAlex
- W1966782526
- Cited by
- 622
- References
- 26
- FWCI
- 9.24
- Type
- journal article
- Language
- en
- Open access
- green
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- Torriani, S., Felis, G. E., & Dellaglio, F. (2001). Differentiation of Lactobacillus plantarum, L. pentosus, and L. paraplantarum by recA Gene Sequence Analysis and Multiplex PCR Assay with recA Gene-Derived Primers. Applied and Environmental Microbiology. https://doi.org/10.1128/aem.67.8.3450-3454.2001
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