The DOI matches its Crossref record; 34 of the 34 references it cites were found in a registry or an index.
Laboratory Cultivation of Widespread and PreviouslyUncultured SoilBacteria
Open accessAbstract
Most soil bacteria belong to family-level phylogenetic groups with few or no known cultivated representatives. We cultured a collection of 350 isolates from soil by using simple solid media in petri dishes. These isolates were assigned to 60 family-level groupings in nine bacterial phyla on the basis of a comparative analysis of their 16S rRNA genes. Ninety-three (27%) of the isolates belonged to 20 as-yet-unnamed family-level groupings, many from poorly studied bacterial classes and phyla. They included members of subdivisions 1, 2, 3, and 4 of the phylum Acidobacteria , subdivision 3 of the phylum Verrucomicrobia , subdivision 1 of the phylum Gemmatimonadetes , and subclasses Acidimicrobidae and Rubrobacteridae of the phylum Actinobacteria . In addition, members of 10 new family-level groupings of subclass Actinobacteridae of the phylum Actinobacteria and classes Alphaproteobacteria , Betaproteobacteria , and Gammaproteobacteria of the phylum Proteobacteria were obtained. The high degree of phylogenetic novelty and the number of isolates affiliated with so-called unculturable groups show that simple cultivation methods can still be developed further to obtain laboratory cultures of many phylogenetically novel soil bacteria.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Nonparametric estimation of the number of classes in a population
Chao, A. (1984) · Scandinavian Journal of Statistics · Cited by 4,265
Impact of Culture-Independent Studies on the Emerging Phylogenetic View of Bacterial Diversity
Hugenholtz, P., Goebel, B. M., & Pace, N. R. (1998) · Journal of Bacteriology · doi:10.1128/jb.180.18.4765-4774.1998 · Cited by 2,428
Self-citation(2006) · CRC Press eBooks · doi:10.1201/9781420015201 · Cited by 1,294
Isolating "Uncultivable" Microorganisms in Pure Culture in a Simulated Natural Environment
Kaeberlein, T., Lewis, K., & Epstein, S. S. (2002) · Science · doi:10.1126/science.1070633 · Cited by 1,237
Zengler, K., Toledo, G., Rappé, M., Elkins, J., Mathur, E. J., Short, J. M., & Keller, M. (2002) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.252630999 · Cited by 796
Janssen, P. H., Yates, P. S., Grinton, B. E., Taylor, P. M., & Sait, M. (2002) · Applied and Environmental Microbiology · doi:10.1128/aem.68.5.2391-2396.2002 · Cited by 731
Self-citationBiodiversity of Denitrifying and Dinitrogen-Fixing Bacteria in an Acid Forest Soil
Rösch, C., Mergel, A., & Bothe, H. (2002) · Applied and Environmental Microbiology · doi:10.1128/aem.68.8.3818-3829.2002 · Cited by 585
Ochsenreiter, T., Selezi, D., Quaiser, A., Bonch‐Osmolovskaya, L., & Schleper, C. (2003) · Environmental Microbiology · doi:10.1046/j.1462-2920.2003.00476.x · Cited by 484
Zhang, H., Sekiguchi, Y., Hanada, S., Hugenholtz, P., Kim, H., Kamagata, Y., & Nakamura, K. (2003) · International Journal of Systematic and Evolutionary Microbiology · doi:10.1099/ijs.0.02520-0 · Cited by 448
Self-citationSait, M., Hugenholtz, P., & Janssen, P. H. (2002) · Environmental Microbiology · doi:10.1046/j.1462-2920.2002.00352.x · Cited by 436
Self-citation
Cited by
Jones, R. T., Robeson, M. S., Lauber, C. L., Hamady, M., Knight, R., & Fierer, N. (2009) · The ISME Journal · doi:10.1038/ismej.2008.127 · Cited by 1,230
Daniel, R. (2005) · Nature Reviews Microbiology · doi:10.1038/nrmicro1160 · Cited by 862
Global Biogeography and Quantitative Seasonal Dynamics of Gemmatimonadetes in Soil
DeBruyn, J. M., Nixon, L. T., Fawaz, M. N., Johnson, A. M., & Radosevich, M. (2011) · Applied and Environmental Microbiology · doi:10.1128/aem.05005-11 · Cited by 624
Eichorst, S. A., Breznak, J. A., & Schmidt, T. M. (2007) · Applied and Environmental Microbiology · doi:10.1128/aem.02140-06 · Cited by 334
The soil metagenome – a rich resource for the discovery of novel natural products
Daniel, R. (2004) · Current Opinion in Biotechnology · doi:10.1016/j.copbio.2004.04.005 · Cited by 334
Metadata
- Authors
- Joseph, S. J., Hugenholtz, P., Sangwan, P., Osborne, C. A., & Janssen, P. H.
- Venue
- Applied and Environmental Microbiology
- Publisher
- American Society for Microbiology
- Published
- 2003-12
- Volume
- 69
- Issue
- 12
- Pages
- 7210-7215
- ISSN
- 0099-2240 (ISSN-L)
- 1098-5336
- OpenAlex
- W2170706168
- Cited by
- 499
- References
- 34
- FWCI
- 27.83
- Type
- journal article
- Language
- en
- Open access
- green
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Laboratory Cultivation of Widespread and PreviouslyUncultured SoilBacteria. https://app.cochat.ai/papers/10.1128/aem.69.12.7210-7215.2003
- The paper
- Joseph, S. J., Hugenholtz, P., Sangwan, P., Osborne, C. A., & Janssen, P. H. (2003). Laboratory Cultivation of Widespread and PreviouslyUncultured SoilBacteria. Applied and Environmental Microbiology. https://doi.org/10.1128/aem.69.12.7210-7215.2003
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
