Cited by 145Oral microbiology and periodontitis researchBacterial biofilms and quorum sensingDental Health and Care Utilization

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Mass Transport of Macromolecules within an In Vitro Model of Supragingival Plaque

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Thurnheer, Thomas, Gmür, Rudolf, Shapiro, Stuart, Guggenheim, Bernhard·2003·Applied and Environmental Microbiology

DOI 10.1128/aem.69.3.1702-1709.2003PMC PMC150054

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Abstract

The aim of this study was to examine the diffusion of macromolecules through an in vitro biofilm model of supragingival plaque. Polyspecies biofilms containing Actinomyces naeslundii , Fusobacterium nucleatum , Streptococcus oralis , Streptococcus sobrinus , Veillonella dispar , and Candida albicans were formed on sintered hydroxyapatite disks and then incubated at room temperature for defined periods with fluorescent markers with molecular weights ranging from 3,000 to 900,000. Subsequent examination by confocal laser scanning microscopy revealed that the mean square penetration depths for all tested macromolecules except immunoglobulin M increased linearly with time, diffusion coefficients being linearly proportional to the cube roots of the molecular weights of the probes (range, 10,000 to 240,000). Compared to diffusion in bulk water, diffusion in the biofilms was markedly slower. The rate of diffusion for each probe appeared to be constant and not a function of biofilm depth. Analysis of diffusion phenomena through the biofilms suggested tortuosity as the most probable explanation for retarded diffusion. Selective binding of probes to receptors present in the biofilms could not explain the observed extent of retardation of diffusion. These results are relevant to oral health, as selective attenuated diffusion of fermentable carbohydrates and acids produced within dental plaque is thought to be essential for the development of carious lesions.

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Authors: Thurnheer, T., Gmür, R., Shapiro, S., & Guggenheim, B.
Affiliations: University of Zurich (CH)
Institute for Oral Microbiology and General Immunology, University of Zürich, CH-8028 Zürich, Switzerland
ORCID: Thurnheer, Thomas
Shapiro, Stuart
Venue: Applied and Environmental Microbiology
Publisher: American Society for Microbiology
Published: 2003-03
Volume: 69
Issue: 3
Pages: 1702-1709
ISSN: 0099-2240 (ISSN-L)
1098-5336
DOI: 10.1128/aem.69.3.1702-1709.2003
PMC: PMC150054
OpenAlex: W2166083475
Cited by: 145
References: 62
Citations by year: 2026: 1 · 2025: 2 · 2024: 3 · 2023: 7 · 2022: 5 · 2021: 10 · 2020: 5 · 2019: 4 · 2018: 8 · 2017: 3 · 2016: 6 · 2015: 11 · 2014: 6 · 2013: 11 · 2012: 6
FWCI: 9.97
Type: journal article
Language: en
Open access: green
europepmc.org
Licence: journals.asm.org/non-commercial-tdm-license (tdm)
Locations: Applied and Environmental Microbiology (publishedVersion)
Europe PMC (PubMed Central) (publishedVersion)
PubMed Central (publishedVersion)
PubMed (publishedVersion)
Zurich Open Repository and Archive (University of Zurich) (acceptedVersion, other-oa)
Zurich Open Repository and Archive (University of Zurich) (submittedVersion, other-oa)
Zurich Open Repository and Archive (University of Zurich)
Funders: Universität Konstanz
Topics: Health Sciences › Dentistry › Periodontics › Oral microbiology and periodontitis research
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bacterial biofilms and quorum sensing
Health Sciences › Dentistry › General Dentistry › Dental Health and Care Utilization
Keywords: supragingival plaque, dental plaque, macromolecular diffusion, diffusion coefficient, molecular weight dependence, tortuosity, fluorescent probes, immunoglobulin M, Actinomyces naeslundii, Fusobacterium nucleatum, Streptococcus sobrinus, Streptococcus oralis, Veillonella dispar, Candida albicans, hydroxyapatite, caries development, oral health
MeSH terms: Actinomyces, Albumins, Candida albicans, Dental Plaque, Dextrans, Diffusion, Fluorescent Dyes, Gingiva, Models, Biological, Veillonella, Fusobacterium nucleatum, Streptococcus sobrinus, Biofilms, Streptococcus oralis, Macromolecular Substances

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Mass Transport of Macromolecules within an In Vitro Model of Supragingival Plaque

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