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A New Alkali-Thermostable Azoreductase from Bacillus sp. Strain SF
Open accessAbstract
A screening for dye-decolorizing alkali-thermophilic microorganisms resulted in a Bacillus sp. strain isolated out of the wastewater drain of a textile finishing company. An NADH-dependent azoreductase of this strain, Bacillus sp. strain SF, was found to be responsible for the decolorization of azo dyes. This enzyme was purified by a combination of ammonium sulfate precipitation and anion-exchange and affinity chromatography and had a molecular mass of 61.6 kDa and an isoelectric point at pH 5.3. The pH optimum of the azoreductase depended on the substrate and was within the range of pHs 8 to 9, while the temperature maximum was reached at 80°C. Decolorization only took place in the absence of oxygen and was enhanced by FAD, which was not consumed during the reaction. A 26% similarity of this azoreductase to chaperonin Cpn60 from a Bacillus sp. was found by peptide mass mapping experiments. Substrate specificities of the azoreductase were studied by using synthesized model substrates based on di-sodium-( R )-benzyl-azo-2,7-dihydroxy-3,6-disulfonyl-naphthaline. Those dyes with NO 2 substituents, especially in the ortho position, were degraded fastest, while analogues with a methyl substitution showed the lowest degradation rates.
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Cited by
Bacterial decolorization and degradation of azo dyes: A review
Saratale, R. G., Saratale, G. D., Chang, J. S., & Govindwar, S. P. (2011) · Journal of the Taiwan Institute of Chemical Engineers · doi:10.1016/j.jtice.2010.06.006 · Cited by 1,344
Enzymatic decolorization and degradation of azo dyes – A review
Singh, R. L., Singh, P. K., & Singh, R. P. (2015) · International Biodeterioration & Biodegradation · doi:10.1016/j.ibiod.2015.04.027 · Cited by 543
Husain, Q. (2006) · Critical Reviews in Biotechnology · doi:10.1080/07388550600969936 · Cited by 429
Current Technologies for Biological Treatment of Textile Wastewater—A Review
Sarayu, K., & Sandhya, S. (2012) · Applied Biochemistry and Biotechnology · doi:10.1007/s12010-012-9716-6 · Cited by 420
Jadhav, J. P., Kalyani, D. C., Telke, A. A., Phugare, S. S., & Govindwar, S. P. (2010) · Bioresource Technology · doi:10.1016/j.biortech.2009.08.027 · Cited by 275
Ben Mansour, H., Houas, I., Montassar, F., Ghedira, K., Barillier, D., Mosrati, R., & Chekir-Ghedira, L. (2012) · Environmental Science and Pollution Research · doi:10.1007/s11356-012-0802-7 · Cited by 78
Sultana, S., Khan, M. D., Sabir, S., Gani, K. M., Oves, M., & Khan, M. Z. (2015) · New Journal of Chemistry · doi:10.1039/c5nj01610j · Cited by 47
Heterogeneous biocatalytic system for effective decolorization of textile dye effluent
Harish, B. S., Thayumanavan, T., Nambukrishnan, V., & Sakthishobana, K. (2023) · 3 Biotech · doi:10.1007/s13205-023-03586-z · Cited by 16
Biological Decolorization of Azo Dyes from Textile Wastewater Effluent by Aspergillus niger
Salem, S., Fouda, A., Mohamed, A., El-Gamal, M., & Talat, M. (2019) · Egyptian Journal of Chemistry · doi:10.21608/ejchem.2019.11720.1747 · Cited by 15
Explorations and Applications of Enzyme-linked Bioremediation of Synthetic Dyes
Ogola, H. J. O., Ashida, H., Ishikawa, T., & Sawa, Y. (2015) · Advances in Bioremediation of Wastewater and Polluted Soil · doi:10.5772/60753 · Cited by 5
Metadata
- Authors
- Maier, J., Kandelbauer, A., Erlacher, A., Cavaco-Paulo, A., & Gübitz, G. M.
- Venue
- Applied and Environmental Microbiology
- Publisher
- American Society for Microbiology
- Published
- 2004-02
- Volume
- 70
- Issue
- 2
- Pages
- 837-844
- ISSN
- 0099-2240 (ISSN-L)
- 1098-5336
- OpenAlex
- W2120151837
- Cited by
- 264
- References
- 0
- FWCI
- 10.87
- Type
- journal article
- Language
- en
- Open access
- green
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