The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Simultaneous Recovery of Extracellular and Intracellular DNA Suitable for Molecular Studies from Marine Sediments
Open accessAbstract
The occurrence of high extracellular DNA concentrations in aquatic sediments (concentrations that are 3 to 4 orders of magnitude greater than those in the water column) might play an important role in biogeochemical cycling, as well as in horizontal gene transfer through natural transformation. Since isolation of extracellular DNA from sediments is a difficult and unsolved task, in this study we developed an efficient procedure to recover simultaneously DNA associated with microbial cells and extracellular DNA from the same sediment sample. This procedure is specifically suitable for studying extracellular DNA because it avoids any contamination with DNA released by cell lysis during handling and extraction. Applying this procedure to different sediment types, we obtained extracellular DNA concentrations that were about 10 to 70 times higher than the intracellular DNA concentrations. Using specific targeted prokaryotic primers, we obtained evidence that extracellular DNA recovered from different sediments did not contain amplifiable 16S rRNA genes. By contrast, using DNA extracted from microbial cells as the template, we always amplified 16S rRNA genes. Although 16S rRNA genes were not detected in extracellular DNA, analyses of the sizes of extracellular DNA indicated the presence of high-molecular-weight fragments that might have contained other gene sequences. This protocol allows investigation of extracellular DNA and its possible participation in natural transformation processes.
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Cites
Lateral gene transfer and the nature of bacterial innovation
Ochman, H., Lawrence, J. G., & Groisman, E. A. (2000) · Nature · doi:10.1038/35012500 · Cited by 3,816
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Turner, C. R., Uy, K. L., & Everhart, R. C. (2015) · Biological Conservation · doi:10.1016/j.biocon.2014.11.017 · Cited by 555
Mao, D., Luo, Y., Mathieu, J., Wang, Q., Feng, L., Mu, Q., Feng, C., & Alvarez, P. J. J. (2014) · Environmental Science & Technology · doi:10.1021/es404280v · Cited by 508
Bacterial extracellular DNA forming a defined network-like structure
Böckelmann, U., Janke, A., Kuhn, R., Neu, T. R., Wecke, J., Lawrence, J. R., & Szewzyk, U. (2006) · FEMS Microbiology Letters · doi:10.1111/j.1574-6968.2006.00361.x · Cited by 176
Zhao, X., Su, H., Xu, W., Hu, X., Xu, Y., Wen, G., & Cao, Y. (2021) · Science of The Total Environment · doi:10.1016/j.scitotenv.2021.146348 · Cited by 36
O'Malley, K., McDonald, W., & McNamara, P. (2023) · Environmental Science: Water Research & Technology · doi:10.1039/d3ew00356f · Cited by 29
Metadata
- Authors
- Corinaldesi, C., Danovaro, R., & Dell'Anno, A.
- Venue
- Applied and Environmental Microbiology
- Publisher
- American Society for Microbiology
- Published
- 2005-01
- Volume
- 71
- Issue
- 1
- Pages
- 46-50
- ISSN
- 0099-2240 (ISSN-L)
- 1098-5336
- OpenAlex
- W2119762376
- Cited by
- 291
- References
- 1
- FWCI
- 8.88
- Type
- journal article
- Language
- en
- Open access
- green
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- CoChat. (2026). Citation check: Simultaneous Recovery of Extracellular and Intracellular DNA Suitable for Molecular Studies from Marine Sediments. https://app.cochat.ai/papers/10.1128/aem.71.1.46-50.2005
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- Corinaldesi, C., Danovaro, R., & Dell'Anno, A. (2005). Simultaneous Recovery of Extracellular and Intracellular DNA Suitable for Molecular Studies from Marine Sediments. Applied and Environmental Microbiology. https://doi.org/10.1128/aem.71.1.46-50.2005
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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