Horizontal gene transfer to endogenous endophytic bacteria from poplar improves phytoremediation of toluene
Open accessAbstract
Poplar, a plant species frequently used for phytoremediation of groundwater contaminated with organic solvents, was inoculated with the endophyte Burkholderia cepacia VM1468. This strain, whose natural host is yellow lupine, contains the pTOM-Bu61 plasmid coding for constitutively expressed toluene degradation. Noninoculated plants or plants inoculated with the soil bacterium B. cepacia Bu61(pTOM-Bu61) were used as controls. Inoculation of poplar had a positive effect on plant growth in the presence of toluene and reduced the amount of toluene released via evapotranspiration. These effects were more dramatic for VM1468, the endophytic strain, than for Bu61. Remarkably, none of the strains became established at detectable levels in the endophytic community, but there was horizontal gene transfer of pTOM-Bu61 to different members of the endogenous endophytic community, both in the presence and in the absence of toluene. This work is the first report of in planta horizontal gene transfer among plant-associated endophytic bacteria and demonstrates that such transfer could be used to change natural endophytic microbial communities in order to improve the remediation of environmental insults.
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Exploring and exploiting the vast metabolic potential of microorganisms enables more efficient degradation of several complex organic compounds.
“Exploring and exploiting the vast metabolic potential of microorganisms (oxidative and peroxidative enzymes in fungi and bacteria, surfactants and alkane dehydrogenases in bacteria) enables more efficient degradation of several complex organic compounds (Taghavi et al., 2005;Barac et al., 2004).”
From Intensify production, transform biomass to energy and novel goods and protect soils in Europe—A vision how to mobilize marginal lands (2018) · cites this paper as Taghavi et al., 2005
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- Authors
- Taghavi, S., Barac, T., Greenberg, B., Borremans, B., Vangronsveld, J., & van der Lelie, D.
- Affiliations
- Brookhaven National Laboratory (US)
- Transnational University Limburg (BE)
- Flemish Institute for Technological Research (BE)
- Brookhaven National Laboratory, Biology Department, Building 463, Upton, New York 11973-5000
- ORCID
- Vangronsveld, Jaco
- Venue
- Applied and Environmental Microbiology
- Publisher
- American Society for Microbiology
- Published
- 2005-12
- Volume
- 71
- Issue
- 12
- Pages
- 8500-8505
- ISSN
- 0099-2240 (ISSN-L)
- 1098-5336
- PMC
- PMC1317371
- OpenAlex
- W2132927938
- Cited by
- 358
- References
- 24
- Citations by year
- 2026: 5 · 2025: 9 · 2024: 5 · 2023: 22 · 2022: 20 · 2021: 25 · 2020: 22 · 2019: 26 · 2018: 22 · 2017: 27 · 2016: 19 · 2015: 19 · 2014: 35 · 2013: 23 · 2012: 13
- FWCI
- 5.98
- Type
- journal article
- Language
- en
- Open access
- green
- europepmc.org
- Locations
- Applied and Environmental Microbiology (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Document Server@UHasselt (UHasselt) (submittedVersion)
- Topics
- Physical Sciences › Environmental Science › Pollution › Microbial bioremediation and biosurfactants
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant-Microbe Interactions and Immunity
- Life Sciences › Agricultural and Biological Sciences › Ecology, Evolution, Behavior and Systematics › Plant and fungal interactions
- Keywords
- horizontal gene transfer, endophytic bacteria, poplar, toluene degradation, phytoremediation, Burkholderia cepacia, endophytic microbial communities, groundwater contamination, plant growth promotion, evapotranspiration
- MeSH terms
- Biodegradation, Environmental, Soil Microbiology, Toluene, Polymerase Chain Reaction, Burkholderia cepacia, DNA Primers, Plant Leaves, Plant Roots, Plant Stems, Gene Transfer, Horizontal, Populus
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