Influence of Growth Phase on Adhesion Kinetics of Escherichia coli D21g
Open accessAbstract
The influence of bacterial growth stage and the evolution of surface macromolecules on cell adhesion have been examined by using a mutant of Escherichia coli K-12. To better understand the adhesion kinetics of bacteria in the mid-exponential and stationary growth phases under flow conditions, deposition experiments were conducted in a well-controlled radial stagnation point flow (RSPF) system. Complementary cell characterization techniques were conducted in combination with the RSPF experiments to evaluate the hydrophobicity, electrophoretic mobility, size, and titratable surface charge of the cells in the two growth phases considered. It was observed that cells in stationary phase were notably more adhesive than those in mid-exponential phase. This behavior is attributed to the high degree of local charge heterogeneity on the outer membranes of stationary-phase cells, which results in decreased electrostatic repulsion between the cells and a quartz surface. The mid-exponential-phase cells, on the other hand, have a more uniform charge distribution on the outer membrane, resulting in greater electrostatic repulsion and, subsequently, less adhesion. Our results suggest that the macromolecules responsible for this phenomenon are outer membrane-bound proteins and lipopolysaccharide-associated functional groups.
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The complex movement of E. coli hardly affects the overall collision frequency.
“This result implies that the complex movement of E. coli hardly affects the overall collision frequency.25”
From Label-Free Detection of Single Living Bacteria via Electrochemical Collision Event (2016) · cites this paper as [25]
The Results and Discussion report virtually identical bacterial transfer rates for two growth phases under favorable conditions, but attribute this finding to the small difference in cell size. The copy does not address whether E. coli’s complex movement affects overall collision frequency (searched: movement, motility, swimming, collision frequency). (LLM, conf=0.72)
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- Authors
- Walker, S. L., Hill, J. E., Redman, J. A., & Elimelech, M.
- Affiliations
- University of California, Riverside (US)
- Yale University (US)
- California State University System (US)
- Corresponding author
- Walker, Sharon L.
- Venue
- Applied and Environmental Microbiology
- Publisher
- American Society for Microbiology
- Published
- 2005-06
- Volume
- 71
- Issue
- 6
- Pages
- 3093-3099
- ISSN
- 0099-2240 (ISSN-L)
- 1098-5336
- PMC
- PMC1151849
- OpenAlex
- W2107504187
- Cited by
- 190
- References
- 72
- Citations by year
- 2026: 2 · 2025: 4 · 2024: 6 · 2023: 5 · 2022: 3 · 2021: 7 · 2020: 5 · 2019: 12 · 2018: 7 · 2017: 7 · 2016: 16 · 2015: 11 · 2014: 11 · 2013: 10 · 2012: 11
- FWCI
- 7.29
- Type
- journal article
- Language
- en
- Open access
- green
- europepmc.org
- Locations
- Applied and Environmental Microbiology (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Endocrinology › Escherichia coli research studies
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bacterial biofilms and quorum sensing
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Keywords
- bacterial adhesion, Escherichia coli, growth phase, stationary phase, exponential growth phase, adhesion kinetics, surface charge heterogeneity, outer membrane, lipopolysaccharide, outer membrane proteins, hydrophobicity, electrophoretic mobility, quartz surface, electrostatic repulsion
- MeSH terms
- Bacterial Adhesion, Kinetics, Quartz, Surface Properties, Escherichia coli K12, Static Electricity, Hydrophobic and Hydrophilic Interactions
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