The DOI matches its Crossref record; 37 of the 37 references it cites were found in a registry or an index.
Hyaluronic Acid Production in Bacillus subtilis
Open accessAbstract
The hasA gene from Streptococcus equisimilis , which encodes the enzyme hyaluronan synthase, has been expressed in Bacillus subtilis , resulting in the production of hyaluronic acid (HA) in the 1-MDa range. Artificial operons were assembled and tested, all of which contain the hasA gene along with one or more genes encoding enzymes involved in the synthesis of the UDP-precursor sugars that are required for HA synthesis. It was determined that the production of UDP-glucuronic acid is limiting in B. subtilis and that overexpressing the hasA gene along with the endogenous tuaD gene is sufficient for high-level production of HA. In addition, the B. subtilis -derived material was shown to be secreted and of high quality, comparable to commercially available sources of HA.
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Cites
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The many ways to cleave hyaluronan
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Masquerading microbial pathogens: capsular polysaccharides mimic host-tissue molecules
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Print Me An Organ! Why We Are Not There Yet
Ng, W. L., Chua, C. K., & Shen, Y.-F. (2019) · Progress in Polymer Science · doi:10.1016/j.progpolymsci.2019.101145 · Cited by 287
Bioactives from probiotics for dermal health: functions and benefits
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Metadata
- Authors
- Widner, B., Behr, R., Von Dollen, S., Tang, M., Heu, T., Sloma, A., Sternberg, D., DeAngelis, P. L., Weigel, P. H., & Brown, S.
- Venue
- Applied and Environmental Microbiology
- Publisher
- American Society for Microbiology
- Published
- 2005-07
- Volume
- 71
- Issue
- 7
- Pages
- 3747-3752
- ISSN
- 0099-2240 (ISSN-L)
- 1098-5336
- OpenAlex
- W2100491597
- Cited by
- 276
- References
- 37
- FWCI
- 2.60
- Type
- journal article
- Language
- en
- Open access
- green
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- Widner, B., Behr, R., Von Dollen, S., Tang, M., Heu, T., Sloma, A., Sternberg, D., DeAngelis, P. L., Weigel, P. H., & Brown, S. (2005). Hyaluronic Acid Production in Bacillus subtilis. Applied and Environmental Microbiology. https://doi.org/10.1128/aem.71.7.3747-3752.2005
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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