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Distinguishing Nitrous Oxide Production from Nitrification and Denitrification on the Basis of Isotopomer Abundances
Open accessAbstract
The intramolecular distribution of nitrogen isotopes in N 2 O is an emerging tool for defining the relative importance of microbial sources of this greenhouse gas. The application of intramolecular isotopic distributions to evaluate the origins of N 2 O, however, requires a foundation in laboratory experiments in which individual production pathways can be isolated. Here we evaluate the site preferences of N 2 O produced during hydroxylamine oxidation by ammonia oxidizers and by a methanotroph, ammonia oxidation by a nitrifier, nitrite reduction during nitrifier denitrification, and nitrate and nitrite reduction by denitrifiers. The site preferences produced during hydroxylamine oxidation were 33.5 ± 1.2‰, 32.5 ± 0.6‰, and 35.6 ± 1.4‰ for Nitrosomonas europaea , Nitrosospira multiformis , and Methylosinus trichosporium , respectively, indicating similar site preferences for methane and ammonia oxidizers. The site preference of N 2 O from ammonia oxidation by N. europaea (31.4 ± 4.2‰) was similar to that produced during hydroxylamine oxidation (33.5 ± 1.2‰) and distinct from that produced during nitrifier denitrification by N. multiformis (0.1 ± 1.7‰), indicating that isotopomers differentiate between nitrification and nitrifier denitrification. The site preferences of N 2 O produced during nitrite reduction by the denitrifiers Pseudomonas chlororaphis and Pseudomonas aureofaciens (−0.6 ± 1.9‰ and −0.5 ± 1.9‰, respectively) were similar to those during nitrate reduction (−0.5 ± 1.9‰ and −0.5 ± 0.6‰, respectively), indicating no influence of either substrate on site preference. Site preferences of ∼33‰ and ∼0‰ are characteristic of nitrification and denitrification, respectively, and provide a basis to quantitatively apportion N 2 O.
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Cited by
Wunderlin, P., Mohn, J., Joss, A., Emmenegger, L., & Siegrist, H. (2012) · Water Research · doi:10.1016/j.watres.2011.11.080 · Cited by 573
The isotopic anatomies of molecules and minerals
Eiler, J. M. (2013) · Annual Review of Earth and Planetary Sciences · doi:10.1146/annurev-earth-042711-105348 · Cited by 96
Wei, J., Zhou, M., Vereecken, H., & Brüggemann, N. (2017) · Rapid Communications in Mass Spectrometry · doi:10.1002/rcm.7912 · Cited by 18
Metadata
- Authors
- Sutka, R. L., Ostrom, N. E., Ostrom, P. H., Breznak, J. A., Gandhi, H., Pitt, A. J., & Li, F.
- Venue
- Applied and Environmental Microbiology
- Publisher
- American Society for Microbiology
- Published
- 2006-01
- Volume
- 72
- Issue
- 1
- Pages
- 638-644
- ISSN
- 0099-2240 (ISSN-L)
- 1098-5336
- OpenAlex
- W2133813560
- Cited by
- 531
- References
- 0
- FWCI
- 27.98
- Type
- journal article
- Language
- en
- Open access
- green
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- CoChat. (2026). Citation check: Distinguishing Nitrous Oxide Production from Nitrification and Denitrification on the Basis of Isotopomer Abundances. https://app.cochat.ai/papers/10.1128/aem.72.1.638-644.2006
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- Sutka, R. L., Ostrom, N. E., Ostrom, P. H., Breznak, J. A., Gandhi, H., Pitt, A. J., & Li, F. (2006). Distinguishing Nitrous Oxide Production from Nitrification and Denitrification on the Basis of Isotopomer Abundances. Applied and Environmental Microbiology. https://doi.org/10.1128/aem.72.1.638-644.2006
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