Large variability in CO 2 and N 2 O emissions and in 15 N site preference of N 2 O from reactions of nitrite with lignin and its derivatives at different pH
Abstract
Rationale Chemodenitrification is an important N 2 O source in soil; however, knowledge about the production of CO 2 and N 2 O from abiotic nitrite‐SOM reactions, especially the N 2 O isotopic signatures (intramolecular 15 N site preference (SP), and δ 15 N bulk and δ 18 O values), is quite limited at present. Methods N 2 O and CO 2 emissions from chemical reactions of nitrite with lignin products were determined with gas chromatography, and their response surfaces as a function of pH from 3 to 6 and nitrite concentration from 0.1 to 0.5 mM were explored with polynomial regression. The intramolecular 15 N distribution of N 2 O, as well as δ 15 N bulk and δ 18 O values, were measured with an isotope ratio mass spectrometer coupled to an online pre‐concentration unit. The variability in N 2 O SP values was tested from pH 3 to 5, and for nitrite concentrations from 0.3 to 0.5 mM. Results Both CO 2 and N 2 O emissions varied largely with pH and the structure of lignin products. The highest N 2 O emission occurred at pH 4–5 in 4‐hydroxy‐3,5‐dimethoxybenzaldehyde and 4‐hydroxy‐3,5‐dimethoxybenzoic acid treatments, and at pH 3 in the treatments with lignin, 4‐hydroxy‐3‐methoxybenzaldehyde, 4‐hydroxy‐3‐methoxybenzoic acid, 4‐hydroxybenzaldehyde, and 4‐hydroxybenzoic acid. A wide range of N 2 O SP values (11.9–37.4‰), which was pH dependent and not distinguishable from microbial pathways, was observed at pH 3–5. The δ 15 N bulk and δ 18 O values of N 2 O were both in a similar range to that reported for fungal denitrification and bacterial denitrification. Conclusions These results present the first characterization of the isotopic composition of N 2 O from chemodenitrification in pure chemical assays. Chemical reactions of nitrite with lignin are pH‐dependent and associated with substantial CO 2 and N 2 O emissions. The SP values of N 2 O derived from chemodenitrification were neither distinguishable from the biotic pathways nor remained stable with varying pH. Therefore, the use of N 2 O isotopic signatures for source partitioning is restricted when chemodenitrification is contributing significantly to N 2 O emission.
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Less prominent ways of abiotic N immobilization were reported, for example the reaction of nitrite with HCA-derived lignin or its derivatives.
“In addition to biotic N immobilization and N adsorption in soil, also less prominent ways of abiotic N immobilization were reported (Miyajima, 2015), e.g., the reaction of nitrite with HCA-derived lignin or its derivatives (Wei et al., 2017).”
From Potential of Wheat Straw, Spruce Sawdust, and Lignin as High Organic Carbon Soil Amendments to Improve Agricultural Nitrogen Retention Capacity: An Incubation Study (2018) · cites this paper as Wei et al., 2017
Lignin was reported as a matrix of chemical N fixation, particularly if reactive N forms like nitrite are present.
“Also lignin was reported as matrix of chemical N fixation (Wei et al., 2017), particularly if reactive N forms like nitrite are present as demonstrated also in our study (Figure 1F).”
From Potential of Wheat Straw, Spruce Sawdust, and Lignin as High Organic Carbon Soil Amendments to Improve Agricultural Nitrogen Retention Capacity: An Incubation Study (2018) · cites this paper as Wei et al., 2017
N derived from dead microorganisms, which does not directly serve as substrate for other microorganisms in the decomposer succession, might be further transformed and fixed to recalcitrant organic molecules.
“N derived from dead microorganisms, which does not directly serve as substrate for other microorganisms in the decomposer succession (Bastian et al., 2009), might be further transformed and fixed to recalcitrant organic molecules (Miyajima, 2015; Wei et al., 2017).”
From Potential of Wheat Straw, Spruce Sawdust, and Lignin as High Organic Carbon Soil Amendments to Improve Agricultural Nitrogen Retention Capacity: An Incubation Study (2018) · cites this paper as Wei et al., 2017
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- Authors
- Wei, J., Zhou, M., Vereecken, H., & Brüggemann, N.
- Affiliations
- Forschungszentrum Jülich (DE)
- Corresponding author
- Wei, Jing
- Venue
- Rapid Communications in Mass Spectrometry
- Publisher
- Wiley
- Published
- 2017-07-10
- Volume
- 31
- Issue
- 16
- Pages
- 1333-1343
- ISSN
- 0951-4198 (ISSN-L)
- 1097-0231
- OpenAlex
- W2619181884
- Cited by
- 18
- References
- 46
- Citations by year
- 2026: 1 · 2023: 1 · 2022: 2 · 2021: 3 · 2020: 5 · 2019: 5 · 2018: 1
- FWCI
- 3.30
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Rapid Communications in Mass Spectrometry (publishedVersion)
- PubMed (publishedVersion)
- Funders
- German Federal Ministry of Science and Education (INPLAMINT (FKZ 031A561A))
- Chinese Scholarship Council (201406890023)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Soil Science › Soil Carbon and Nitrogen Dynamics
- Physical Sciences › Earth and Planetary Sciences › Atmospheric Science › Atmospheric chemistry and aerosols
- Physical Sciences › Earth and Planetary Sciences › Geochemistry and Petrology › Groundwater and Isotope Geochemistry
- Keywords
- nitrite, lignin, lignin derivatives, CO2 emissions, pH dependence, nitrite content, soil nitrogen cycling, lignin oxidation products, 4-hydroxybenzaldehyde
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