Cited by 56Mast cells and histamineAsthma and respiratory diseasesAllergic Rhinitis and Sensitization

Induction of interleukin-4 and interleukin-5 expression in mast cells is inhibited by glucocorticoids

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Sewell, William A., Scurr, Lyndee L., Orphanides, Helen, Kinder, Simon, Ludowyke, Russell I.·1998·Clinical Diagnostic Laboratory Immunology

Abstract

Inflammation in asthma and other allergic diseases is characterized by excessive production of immunoglobulin E (IgE) and the influx of leukocytes, especially eosinophils. Interleukin 4 (IL-4) and IL-5 are essential for IgE production and eosinophilia, respectively, and are produced by mast cells in allergic conditions, for which glucocorticoids are widely used therapeutically. We assessed the effect of glucocorticoids on IL-4 and IL-5 mRNA production by the RBL-2H3 cell line, an analog of mucosal mast cells. IL-4 and IL-5 mRNAs were induced by an antigen that is used to cross-link receptor bound IgE, by calcium ionophore, or by ionophore with phorbol ester and were markedly inhibited by dexamethasone. In cells activated with ionophore and phorbol ester, 10(-6) M dexamethasone reduced the IL-4 and IL-5 mRNA levels to only 12.8 and 5.7%, respectively, of those in cells without dexamethasone, and 10(-9) M dexamethasone caused reductions to 27 and 56%, respectively. Hydrocortisone at 10(-6) and 10(-7) M almost completely inhibited IL-4 and IL-5 mRNA production. Dexamethasone was markedly inhibitory even if it was added after the cells were activated, provided that it was present in the cultures for at least 1.5 h. These studies indicate that the expression of IL-4 and IL-5 mRNAs by mast cells is highly sensitive to glucocorticoids. The data suggest that these inhibitory effects may contribute to the clinical efficacy of glucocorticoids in the therapy of allergic diseases.

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Crossref · 10.1128/cdli.5.1.18-23.1998

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Authors
Sewell, W. A., Scurr, L. L., Orphanides, H., Kinder, S., & Ludowyke, R. I.
Affiliations
UNSW Sydney (AU)
Venue
Clinical Diagnostic Laboratory Immunology
Published
1998-01
Volume
5
Issue
1
Pages
18-23
ISSN
  • 1071-412X (ISSN-L)
  • 1098-6588
OpenAlex
W1816680223
Cited by
56
References
45
Citations by year
2026: 3 · 2025: 1 · 2024: 1 · 2023: 1 · 2022: 2 · 2021: 1 · 2020: 2 · 2019: 5 · 2018: 5 · 2017: 1 · 2015: 1 · 2013: 2 · 2012: 1
FWCI
0.72
Type
journal article
Language
en
Open access
Locations
  • Clinical and Diagnostic Laboratory Immunology (publishedVersion)
  • Europe PMC (PubMed Central) (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Immunology and Microbiology › Immunology › Mast cells and histamine
  • Health Sciences › Medicine › Physiology › Asthma and respiratory diseases
  • Health Sciences › Medicine › Immunology and Allergy › Allergic Rhinitis and Sensitization
Keywords
IL-4, IL-5, mast cells, glucocorticoids, dexamethasone, cortisol, RBL-2H3 cells, IgE production, eosinophilia, allergic inflammation, asthma, cytokine mRNA expression, phorbol ester, calcium ionophore
MeSH terms
Animals, Anti-Inflammatory Agents, Dexamethasone, Glucocorticoids, Hydrocortisone, Mast Cells, RNA, Messenger, Time Factors, Tumor Cells, Cultured, Interleukin-4, Interleukin-5, Gene Expression, Rats

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