The DOI matches its Crossref record; 112 of the 126 references it cites were found in a registry or an index, and 1 are not checked yet.
Multiplex PCR: Optimization and Application in Diagnostic Virology
Open accessAbstract
SUMMARY: PCR has revolutionized the field of infectious disease diagnosis. To overcome the inherent disadvantage of cost and to improve the diagnostic capacity of the test, multiplex PCR, a variant of the test in which more than one target sequence is amplified using more than one pair of primers, has been developed. Multiplex PCRs to detect viral, bacterial, and/or other infectious agents in one reaction tube have been described. Early studies highlighted the obstacles that can jeopardize the production of sensitive and specific multiplex assays, but more recent studies have provided systematic protocols and technical improvements for simple test design. The most useful of these are the empirical choice of oligonucleotide primers and the use of hot start-based PCR methodology. These advances along with others to enhance sensitivity and specificity and to facilitate automation have resulted in the appearance of numerous publications regarding the application of multiplex PCR in the diagnosis of infectious agents, especially those which target viral nucleic acids. This article reviews the principles, optimization, and application of multiplex PCR for the detection of viruses of clinical and epidemiological importance.
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“These results were probably due to a preferential amplification of one DNA template over the other, a frequent phenomenon when using multiplex PCRs [28].”
From Dirofilaria immitis and D. repens show circadian co-periodicity in naturally co-infected dogs (2017) · cites this paper as [28]
Citations
Cites
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Deletion screening of the Duchenne muscular dystrophy locus via multiplex DNA amplification
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Cited by
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Kaminski, M. M., Abudayyeh, O. O., Gootenberg, J. S., Zhang, F., & Collins, J. J. (2021) · Nature Biomedical Engineering · doi:10.1038/s41551-021-00760-7 · Cited by 1,293
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Point-of-Care Diagnostics for Global Health
Yager, P., Domingo, G. J., & Gerdes, J. (2008) · Annual Review of Biomedical Engineering · doi:10.1146/annurev.bioeng.10.061807.160524 · Cited by 1,076
Current trends in microsatellite genotyping
Guichoux, E., Lagache, L., Wagner, S., Chaumeil, P., Léger, P., Lepais, O., Lepoittevin, C., Malausa, T., Revardel, E., Salin, F., et al. (2011) · Molecular Ecology Resources · doi:10.1111/j.1755-0998.2011.03014.x · Cited by 942
Multiplex polymerase chain reaction: A practical approach
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Metadata
- Authors
- Elnifro, E. M., Ashshi, A. M., Cooper, R. J., & Klapper, P. E.
- Affiliations
- University of Manchester (GB)
- <!--label omitted: 1-->School of Medicine, The University of Manchester,1 and
- ORCID
- Klapper, Paul E.
- Venue
- Clinical Microbiology Reviews
- Publisher
- American Society for Microbiology
- Published
- 2000-10
- Volume
- 13
- Issue
- 4
- Pages
- 559-570
- ISSN
- 0893-8512 (ISSN-L)
- 1098-6618
- PMC
- PMC88949
- OpenAlex
- W2137089963
- Cited by
- 964
- References
- 126
- Citations by year
- 2026: 54 · 2025: 58 · 2024: 67 · 2023: 60 · 2022: 50 · 2021: 57 · 2020: 51 · 2019: 38 · 2018: 29 · 2017: 37 · 2016: 22 · 2015: 43 · 2014: 30 · 2013: 46 · 2012: 40
- FWCI
- 0.55
- Type
- journal article
- Language
- en
- Open access
- green
- europepmc.org
- Locations
- Clinical Microbiology Reviews (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Research Explorer (The University of Manchester) (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Molecular Biology Techniques and Applications
- Physical Sciences › Engineering › Biomedical Engineering › Biosensors and Analytical Detection
- Health Sciences › Medicine › Microbiology (medical) › Bacterial Identification and Susceptibility Testing
- Keywords
- multiplex PCR, PCR optimization, diagnostic virology, virus detection, primer design, hot-start PCR, infectious disease diagnosis, viral nucleic acids, assay sensitivity and specificity
- MeSH terms
- Humans, Virology, Virus Diseases, Viruses, Polymerase Chain Reaction
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