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RNA Interference Silencing of a Major Lipid Droplet Protein Affects Lipid Droplet Size in Chlamydomonas reinhardtii
Open accessAbstract
Eukaryotic cells store oils in the chemical form of triacylglycerols in distinct organelles, often called lipid droplets. These dynamic storage compartments have been intensely studied in the context of human health and also in plants as a source of vegetable oils for human consumption and for chemical or biofuel feedstocks. Many microalgae accumulate oils, particularly under conditions limiting to growth, and thus have gained renewed attention as a potentially sustainable feedstock for biofuel production. However, little is currently known at the cellular or molecular levels with regard to oil accumulation in microalgae, and the structural proteins and enzymes involved in the biogenesis, maintenance, and degradation of algal oil storage compartments are not well studied. Focusing on the model green alga Chlamydomonas reinhardtii , the accumulation of triacylglycerols and the formation of lipid droplets during nitrogen deprivation were investigated. Mass spectrometry identified 259 proteins in a lipid droplet-enriched fraction, among them a major protein, tentatively designated major lipid droplet protein (MLDP). This protein is specific to the green algal lineage of photosynthetic organisms. Repression of MLDP gene expression using an RNA interference approach led to increased lipid droplet size, but no change in triacylglycerol content or metabolism was observed.
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Cited by
Biodiesel from algae: challenges and prospects
Scott, S. A., Davey, M. P., Dennis, J. S., Horst, I., Howe, C. J., Lea-Smith, D. J., & Smith, A. G. (2010) · Current Opinion in Biotechnology · doi:10.1016/j.copbio.2010.03.005 · Cited by 1,183
Genetic Engineering of Algae for Enhanced Biofuel Production
Radakovits, R., Jinkerson, R. E., Darzins, A., & Posewitz, M. C. (2010) · Eukaryotic Cell · doi:10.1128/ec.00364-09 · Cited by 1,142
Advances and perspectives in using microalgae to produce biodiesel
Amaro, H. M., Guedes, A. C., & Malcata, F. X. (2011) · Applied Energy · doi:10.1016/j.apenergy.2010.12.014 · Cited by 572
Miller, R., Wu, G., Deshpande, R. R., Vieler, A., Gärtner, K., Li, X., Moellering, E. R., Zäuner, S., Cornish, A. J., Liu, B., et al. (2010) · PLANT PHYSIOLOGY · doi:10.1104/pp.110.165159 · Cited by 511
Self-citationThe lipid biochemistry of eukaryotic algae
Li-Beisson, Y., Thelen, J. J., Fedosejevs, E., & Harwood, J. L. (2019) · Progress in Lipid Research · doi:10.1016/j.plipres.2019.01.003 · Cited by 453
Metadata
- Authors
- Moellering, E. R., & Benning, C.
- Venue
- Eukaryotic Cell
- Publisher
- American Society for Microbiology
- Published
- 2010-01
- Volume
- 9
- Issue
- 1
- Pages
- 97-106
- ISSN
- 1535-9778 (ISSN-L)
- 1535-9786
- OpenAlex
- W2144640901
- Cited by
- 399
- References
- 2
- FWCI
- 9.51
- Type
- journal article
- Language
- en
- Open access
- diamond
- Licence
- journals.asm.org/non-commercial-tdm-license (tdm)
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- CoChat. (2026). Citation check: RNA Interference Silencing of a Major Lipid Droplet Protein Affects Lipid Droplet Size in Chlamydomonas reinhardtii. https://app.cochat.ai/papers/10.1128/ec.00203-09
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- Moellering, E. R., & Benning, C. (2010). RNA Interference Silencing of a Major Lipid Droplet Protein Affects Lipid Droplet Size in Chlamydomonas reinhardtii. Eukaryotic Cell. https://doi.org/10.1128/ec.00203-09
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