CoChat
Sign Up
Cited by 56Fungal and yeast genetics researchGenomics and Chromatin DynamicsBacterial Genetics and Biotechnology

Combinatorial Repression of the Hypoxic Genes of Saccharomyces cerevisiae by DNA Binding Proteins Rox1 and Mot3

Open access
Klinkenberg, Lee G., Mennella, Thomas A., Luetkenhaus, Katharina, Zitomer, Richard S.·2005·Eukaryotic Cell
DOI 10.1128/ec.4.4.649-660.2005PMC PMC1087805
Open this paper in CoChat — free account

Abstract

The hypoxic genes of Saccharomyces cerevisiae are transcriptionally repressed during aerobic growth through recruitment of the Ssn6/Tup1 general repression complex by the DNA binding protein Rox1. A second DNA binding protein Mot3 enhances repression of some hypoxic genes. Previous studies characterized the role of Mot3 at the hypoxic ANB1 gene as promoting synergy among one Mot3 site and two Rox1 sites comprising operator A of that gene. Here we studied the role of Mot3 in enhancing repression by Rox1 at another hypoxic gene, HEM13, which is less strongly regulated than ANB1 and has a very different arrangement of Rox1 and Mot3 binding sites. By assessing the effects of deleting Rox1 and Mot3 sites individually and in combination, we found that the major repression of HEM13 occurred through three Mot3 sites closely spaced with a single Rox1 site. While the Mot3 sites functioned additively, they enhanced repression by the single Rox1 site, and the presence of Rox1 enhanced the additive effects of the Mot3 sites. In addition, using a Rox1-Ssn6 fusion protein, we demonstrated that Mot3 enhances Rox1 repression through helping recruit the Ssn6/Tup1 complex. Chromatin immunoprecipitation assays indicated that Rox1 stabilized Mot3 binding to DNA. Integrating these results, we were able to devise a set of rules that govern the combinatorial interactions between Rox1 and Mot3 to achieve differential repression.

Integrity signals

Registry

Crossref · 10.1128/ec.4.4.649-660.2005

Cites

50 of 50 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

We couldn’t retrieve this paper’s full text, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

  • Rox1 and Mot3 regulatory factors act synergistically to achieve stringent repression of target genes.

    “Rox1 and Mot3 regulatory factors act synergistically to achieve stringent repression of target genes [89,90].”

    From Regulation of Ergosterol Biosynthesis in Saccharomyces cerevisiae (2020) · cites this paper as [90]

  • Mot3 represses the expression of hypoxic, DAN/TIR/PAU and ERG genes including ERG2, ERG6 and ERG9.

    “In addition, to limit Ecm22 function [75], the transcriptional factor Mot3 also represses the expression of hypoxic, DAN/TIR/PAU and ERG genes including ERG2, ERG6 and ERG9 (Figure 3A) [86,89,90,91,92,93].”

    From Regulation of Ergosterol Biosynthesis in Saccharomyces cerevisiae (2020) · cites this paper as [90]

Cites

Flagged first, then by citations

Cited by

Sorted by citations

Related

Authors
Klinkenberg, L. G., Mennella, T. A., Luetkenhaus, K., & Zitomer, R. S.
Affiliations
University at Albany, State University of New York (US)
Corresponding author
Zitomer, Richard S.
ORCID
Klinkenberg, Lee G.
Venue
Eukaryotic Cell
Publisher
American Society for Microbiology
Published
2005-04
Volume
4
Issue
4
Pages
649-660
ISSN
  • 1535-9778 (ISSN-L)
  • 1535-9786
DOI
10.1128/ec.4.4.649-660.2005
PMC
PMC1087805
OpenAlex
W2116179409
Cited by
56
References
50
Citations by year
2025: 2 · 2024: 1 · 2022: 3 · 2021: 3 · 2020: 3 · 2019: 1 · 2018: 1 · 2017: 3 · 2016: 2 · 2015: 2 · 2014: 1 · 2013: 2 · 2012: 6
FWCI
1.38
Type
journal article
Language
en
Open access
Licence
journals.asm.org/non-commercial-tdm-license (tdm)
Locations
  • Eukaryotic Cell (publishedVersion)
  • Europe PMC (PubMed Central) (publishedVersion)
  • PubMed (publishedVersion)
  • PubMed Central (publishedVersion)
Funders
  • National Institutes of Health
  • National Institute of General Medical Sciences (GM 26061, R01GM026061)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
Keywords
roX1, transcriptional repression, DNA-binding proteins, transcription factor binding sites, Saccharomyces cerevisiae
MeSH terms
Eukaryotic Translation Initiation Factor 5A, Binding Sites, DNA-Binding Proteins, DNA, Fungal, Peptide Initiation Factors, Repressor Proteins, Saccharomyces cerevisiae, Transcription Factors, Transcription, Genetic, Cell Hypoxia, Gene Expression Regulation, Fungal, RNA-Binding Proteins, DNA Primers, Saccharomyces cerevisiae Proteins, Chromatin Immunoprecipitation

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review