The DOI matches its Crossref record; 44 of the 44 references it cites were found in a registry or an index.
Alanine Esters of Enterococcal Lipoteichoic Acid Play a Role in Biofilm Formation and Resistance to Antimicrobial Peptides
Abstract
Enterococcus faecalis is among the predominant causes of nosocomial infections. Surface molecules like d -alanine lipoteichoic acid (LTA) perform several functions in gram-positive bacteria, such as maintenance of cationic homeostasis and modulation of autolytic activities. The aim of the present study was to evaluate the effect of d -alanine esters of teichoic acids on biofilm production and adhesion, autolysis, antimicrobial peptide sensitivity, and opsonic killing. A deletion mutant of the dltA gene was created in a clinical E. faecalis isolate. The absence of d -alanine in the LTA of the dltA deletion mutant was confirmed by nuclear magnetic resonance spectroscopy. The wild-type strain and the deletion mutant did not show any significant differences in growth curve, morphology, or autolysis. However, the mutant produced significantly less biofilm when grown in the presence of 1% glucose (51.1% compared to that of the wild type); adhesion to eukaryotic cells was diminished. The mutant absorbed 71.1% of the opsonic antibodies, while absorption with the wild type resulted in a 93.2% reduction in killing. Sensitivity to several cationic antimicrobial peptides (polymyxin B, colistin, and nisin) was considerably increased in the mutant strain, confirming similar results from other studies of gram-positive bacteria. Our data suggest that the absence of d -alanine in LTA plays a role in environmental interactions, probably by modulating the net negative charge of the bacterial cell surface, and therefore it may be involved in the pathogenesis of this organism.
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Cites
Biofilm formation as microbial development
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Role of Mobile DNA in the Evolution of Vancomycin-Resistant Enterococcus faecalis
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The Enterococcal Surface Protein, Esp, Is Involved in Enterococcus faecalis Biofilm Formation
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Key Role of Teichoic Acid Net Charge in Staphylococcus aureus Colonization of Artificial Surfaces
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How do bacteria resist human antimicrobial peptides?
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Cited by
The rise of the Enterococcus: beyond vancomycin resistance
Arias, C. A., & Murray, B. E. (2012) · Nature Reviews Microbiology · doi:10.1038/nrmicro2761 · Cited by 1,776
Molecular control of bacterial death and lysis
Rice, K. C., & Bayles, K. W. (2008) · Microbiology and Molecular Biology Reviews · doi:10.1128/mmbr.00030-07 · Cited by 362
Action of Antimicrobial Peptides against Bacterial Biofilms
Yasir, M., Willcox, M. D. P., & Dutta, D. (2018) · Materials · doi:10.3390/ma11122468 · Cited by 319
Sakoulas, G., Bayer, A. S., Pogliano, J., Tsuji, B. T., Yang, S.-J., Mishra, N. N., Nizet, V., Yeaman, M. R., & Moise, P. A. (2012) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.05551-11 · Cited by 176
Lactobacillus plantarum lipoteichoic acid inhibits biofilm formation of Streptococcus mutans
Ahn, K. B., Baik, J. E., Park, O.-J., Yun, C.-H., & Han, S. H. (2018) · PLOS ONE · doi:10.1371/journal.pone.0192694 · Cited by 113
Metadata
- Authors
- Fabretti, F., Theilacker, C., Baldassarri, L., Kaczynski, Z., Kropec, A., Holst, O., & Huebner, J.
- Venue
- Infection and Immunity
- Publisher
- American Society for Microbiology
- Published
- 2006-07
- Volume
- 74
- Issue
- 7
- Pages
- 4164-4171
- ISSN
- 0019-9567 (ISSN-L)
- 1098-5522
- OpenAlex
- W2121857077
- Cited by
- 255
- References
- 44
- FWCI
- 3.93
- Type
- journal article
- Language
- en
- Open access
- closed
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- Fabretti, F., Theilacker, C., Baldassarri, L., Kaczynski, Z., Kropec, A., Holst, O., & Huebner, J. (2006). Alanine Esters of Enterococcal Lipoteichoic Acid Play a Role in Biofilm Formation and Resistance to Antimicrobial Peptides. Infection and Immunity. https://doi.org/10.1128/iai.00111-06
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