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Cited by 63

The DOI matches its Crossref record; 18 of the 18 references it cites were found in a registry or an index.

Congo red binding phenotype is associated with hemin binding and increased infectivity of Shigella flexneri in the HeLa cell model

Daskaleros, P A, Payne, S M·1987·Infection and Immunity
DOI 10.1128/iai.55.6.1393-1398.1987
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Abstract

Wild-type isolates of Shigella flexneri bind the dye Congo red from solid media, thus producing red (Crb+) colonies. Mutants which fail to bind the dye produce white colonies (Crb-) and are avirulent in a variety of systems. In S. flexneri the ability to bind Congo red correlates with the ability to bind hemin and protoporphyrin IX. Binding of hemin by Crb+ S. flexneri was observed both in solid media and in liquid assays. Results of competition experiments suggest that Congo red and hemin bind to the same site on the bacterial cell and are retained on the cell surface. Binding of hemin by Crb+ S. flexneri is independent of hemin transport since both Crb+ and Crb- cells can utilize hemin as a sole source of iron. Both Crb- and Crb- organisms were able to grow in HeLa cell lysates, indicating that the gene(s) that is responsible for Congo red binding does not play a role in the acquisition of intracellular heme iron. By using the HeLa cell invasion system, the effect of hemin prebinding on the invasiveness of Crb+ S. flexneri was determined. Crb+ cells which had prebound hemin exhibited increased invasiveness, indicating a possible role for the crb gene product in the initial stages of invasion by S. flexneri.

Overview

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Crossref · 10.1128/iai.55.6.1393-1398.1987

Cites

18 of 18 references verified0 citation errors · 0 not checkable

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Citations

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Metadata

Authors
Daskaleros, P. A., & Payne, S. M.
Venue
Infection and Immunity
Publisher
American Society for Microbiology
Published
1987-06
Volume
55
Issue
6
Pages
1393-1398
ISSN
  • 0019-9567
  • 1098-5522
DOI
10.1128/iai.55.6.1393-1398.1987
Cited by
63
References
18
Language
en
Licence
journals.asm.org/non-commercial-tdm-license (tdm)

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CoChat. (2026). Citation check: Congo red binding phenotype is associated with hemin binding and increased infectivity of Shigella flexneri in the HeLa cell model. https://app.cochat.ai/papers/10.1128/iai.55.6.1393-1398.1987
The paper
Daskaleros, P. A., & Payne, S. M. (1987). Congo red binding phenotype is associated with hemin binding and increased infectivity of Shigella flexneri in the HeLa cell model. Infection and Immunity. https://doi.org/10.1128/iai.55.6.1393-1398.1987

Data sources

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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).

Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.