The DOI matches its Crossref record; 34 of the 34 references it cites were found in a registry or an index.
Immunogenic integral membrane proteins of Borrelia burgdorferi are lipoproteins
Open accessAbstract
The pathogenic spirochete Borrelia burgdorferi contains a set of integral membrane proteins which were selectively extracted into the detergent phase upon solubilization of intact B. burgdorferi with the nonionic detergent Triton X-114. Virtually all of these hydrophobic proteins were recognized by antibodies in pooled sera from patients with chronic Lyme arthritis, demonstrating that proteins partitioning into the detergent phase of Triton X-114 encompass the major B. burgdorferi immunogens. Furthermore, most of these immunogenic proteins, including the previously characterized OspA and OspB membrane antigens, could be biosynthetically labeled when B. burgdorferi was incubated in vitro with [3H]palmitate. The OspA and OspB antigens were radioimmunoprecipitated from [3H]palmitate-labeled detergent-phase proteins with monoclonal antibodies, and [3H]palmitate was recovered unaltered from these proteins after sequential alkaline and acid hydrolyses. The combined results provide formal confirmation that the major B. burgdorferi immunogens extracted by Triton X-114 are lipoproteins. The demonstration that B. burgdorferi integral membrane antigens are lipoproteins may explain the basis of their immunogenicity and may help to improve our understanding of the surface topology of B. burgdorferi.
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Cites
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
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A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION
Bligh, E. G., & Dyer, W. J. (1959) · Canadian Journal of Biochemistry and Physiology · doi:10.1139/o59-099 · Cited by 54,751
Phase separation of integral membrane proteins in Triton X-114 solution.
Bordier, C. (1981) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)69848-0 · Cited by 3,324
Lyme Disease—a Tick-Borne Spirochetosis?
Burgdorfer, W., Barbour, A. G., Hayes, S. F., Benach, J. L., Grunwaldt, E., & Davis, J. P. (1982) · Science · doi:10.1126/science.7043737 · Cited by 2,859
Isolation and cultivation of Lyme disease spirochetes.
Barbour, A. G. (1984) · PubMed Central · Cited by 1,556
Spirochetes Isolated from the Blood of Two Patients with Lyme Disease
Benach, J. L., Bosler, E. M., Hanrahan, J. P., Coleman, J. L., Habicht, G. S., Bast, T. F., Cameron, D. J., Ziegler, J. L., Barbour, A. G., Burgdorfer, W., et al. (1983) · New England Journal of Medicine · doi:10.1056/nejm198303313081302 · Cited by 813
The Clinical Evolution of Lyme Arthritis
STEERE, A. C., SCHOEN, R. T., & TAYLOR, E. (1987) · Annals of Internal Medicine · doi:10.7326/0003-4819-107-5-725 · Cited by 784
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The Early Clinical Manifestations of Lyme Disease
STEERE, A. C., BARTENHAGEN, N. H., CRAFT, J. E., HUTCHINSON, G. J., NEWMAN, J. H., RAHN, D. W., SIGAL, L. H., SPIELER, P. N., STENN, K. S., & MALAWISTA, S. E. (1983) · Annals of Internal Medicine · doi:10.7326/0003-4819-99-1-76 · Cited by 662
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Cited by
Genomic sequence of a Lyme disease spirochaete, Borrelia burgdorferi
Fraser, C. M., Casjens, S., Huang, W. M., Sutton, G. G., Clayton, R., Lathigra, R., White, O., Ketchum, K. A., Dodson, R., Hickey, E. K., et al. (1997) · Nature · doi:10.1038/37551 · Cited by 2,108
Kalish, R. A., Leong, J. M., & Steere, A. C. (1993) · Infection and Immunity · doi:10.1128/iai.61.7.2774-2779.1993 · Cited by 299
Badten, A. J., & Torres, A. G. (2024) · Vaccines · doi:10.3390/vaccines12030313 · Cited by 8
Metadata
- Authors
- Brandt, M. E., Riley, B. S., Radolf, J. D., & Norgard, M. V.
- Venue
- Infection and Immunity
- Publisher
- American Society for Microbiology
- Published
- 1990-04
- Volume
- 58
- Issue
- 4
- Pages
- 983-991
- ISSN
- 0019-9567 (ISSN-L)
- 1098-5522
- OpenAlex
- W1922236261
- Cited by
- 279
- References
- 34
- FWCI
- 21.78
- Type
- journal article
- Language
- en
- Open access
- green
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- Brandt, M. E., Riley, B. S., Radolf, J. D., & Norgard, M. V. (1990). Immunogenic integral membrane proteins of Borrelia burgdorferi are lipoproteins. Infection and Immunity. https://doi.org/10.1128/iai.58.4.983-991.1990
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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