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Cited by 43

The DOI matches its Crossref record; 35 of the 35 references it cites were found in a registry or an index.

Interaction of the fish pathogen Aeromonas salmonicida with rainbow trout macrophages

Garduño, R A, Kay, W W·1992·Infection and Immunity
DOI 10.1128/iai.60.11.4612-4620.1992
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Abstract

A procedure was developed to culture rainbow trout macrophages (M phi) on supported glass coverslips. Using this method and a variety of well-characterized Aeromonas salmonicida strains with normal or altered cell surfaces, we investigated the role of this unusual bacterial surface in the bacterium-M phi interaction. An intact crystalline protein array, the A-layer, mediated adherence of A. salmonicida cells to M phi even in the absence of opsonins. In contrast, unopsonized cells of an A-layer-negative (A-) mutant with a smooth lipopolysaccharide (LPS) layer were unable to interact with M phi. However, this ability was recovered when the A-layer was reconstituted onto the smooth LPS surface of these A- LPS+ cells. Two A. salmonicida mutants possessing the A-layer in different disorganized states had a reduced ability to interact with M phi. A+ cells grown under calcium limitation produced A-layers locked into an alternative conformation which mediated the highest levels of M phi association in the absence of opsonins or any other surface coating. Coating A+ cells with hemin greatly increased their levels of M phi association, and bacterial cells grown on trout blood agar plates also had a dramatic increase in their ability to interact with M phi. Only A+ A. salmonicida cells were highly cytotoxic to trout M phi, especially after being coated with hemin, presumably due to a more focused targeting of the bacterial cell onto the M phi surface and/or into the intracellular regions of the M phi.

Overview

Integrity signals

Registry

Crossref · 10.1128/iai.60.11.4612-4620.1992

Cites

35 of 35 references verified0 citation errors · 0 not checkable

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Verification

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Citations

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Cited by

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Metadata

Authors
Garduño, R. A., & Kay, W. W.
Affiliations
Department of Biochemistry and Microbiology, University of Victoria, British Columbia, Canada.
Venue
Infection and Immunity
Publisher
American Society for Microbiology
Published
1992-11
Volume
60
Issue
11
Pages
4612-4620
ISSN
  • 0019-9567
  • 1098-5522
DOI
10.1128/iai.60.11.4612-4620.1992
Cited by
43
References
35
Language
en
Licence
journals.asm.org/non-commercial-tdm-license (tdm)

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Data sources

Bibliographic data: Semantic Scholar, OpenAlex, Crossref.

Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).

Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.