Human tracheobronchial mucin: purification and binding to Pseudomonas aeruginosa
Open accessAbstract
Colonization of the respiratory tract with Pseudomonas aeruginosa is a serious problem in cystic fibrosis and seriously ill hospitalized patients. Human tracheobronchial mucin (HTBM), the major glycoprotein of human tracheobronchial secretions, is known to interact with this pathogen, which may then be cleared by mucociliary action. However, the mechanism of interaction is not known. To understand this process, pure HTBM was isolated from tracheobronchial secretions of a laryngectomee. Following initial fractionation on Sepharose CL-2B, the HTBM-containing fraction was subjected to reductive methylation and then gel filtration. Pure HTBM was employed in an overlay binding assay to identify the bacterial adhesin(s) and mucin receptors that participate in mucin-P. aeruginosa interactions. An approximately 16-kDa nonpilus protein component(s) of P. aeruginosa was found to be the adhesin(s) for HTBM. The mucin receptor for the 16-kDa component(s) was found in the peptide moiety. This study confirms that P. aeruginosa utilizes the nonpilus adhesin(s) to bind to HTBM. Identification of the specificity of the HTBM-P. aeruginosa interactions can lead to a better understanding of the predominance of P. aeruginosa colonization in individuals with cystic fibrosis.
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- Authors
- Reddy, M. S.
- Affiliations
- University at Buffalo, State University of New York (US)
- Corresponding author
- Reddy, M S
- Venue
- Infection and Immunity
- Publisher
- American Society for Microbiology
- Published
- 1992-04
- Volume
- 60
- Issue
- 4
- Pages
- 1530-1535
- ISSN
- 0019-9567 (ISSN-L)
- 1098-5522
- PMC
- PMC257027
- OpenAlex
- W1920211617
- Cited by
- 48
- References
- 32
- Citations by year
- 2025: 1 · 2022: 1 · 2020: 2 · 2015: 1 · 2014: 2 · 2013: 2
- FWCI
- 2.12
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Infection and Immunity (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health (1r01ai027401-01)
- National Institute of Allergy and Infectious Diseases (AI27401)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bacterial biofilms and quorum sensing
- Health Sciences › Medicine › Pulmonary and Respiratory Medicine › Cystic Fibrosis Research Advances
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Glycosylation and Glycoproteins Research
- Keywords
- Pseudomonas aeruginosa, bacterial adhesion, mucin purification, cystic fibrosis, respiratory tract colonization, mucociliary clearance
- MeSH terms
- Aged, Bacterial Adhesion, Bronchi, Chromatography, Gel, Electrophoresis, Polyacrylamide Gel, Humans, Male, Mucins, Pseudomonas aeruginosa, Trachea, In Vitro Techniques
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