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Cited by 73Barrier Structure and Function StudiesProtease and Inhibitor MechanismsEscherichia coli research studies

A bacterial protease perturbs the paracellular barrier function of transporting epithelial monolayers in culture

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Azghani, A O, Gray, L D, Johnson, A R·1993·Infection and Immunity
DOI 10.1128/iai.61.6.2681-2686.1993PMC PMC280900
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Abstract

Tight junctions between cells and adhesion to the substratum maintain the barrier function of epithelia throughout the body. Damage to the epithelial barrier by microbial products allows penetration of bacteria and promotion of infection. We studied the effects of Pseudomonas elastase (PE) on the barrier function of epithelia by using Madin-Darby canine kidney (MDCK) epithelial cells; these cells form tight junctions (zonula occludens [ZO]) in vitro. PE decreased electrical resistance across the monolayers in a concentration- and time-dependent manner. Immunostaining of selected proteins of the ZO and zonula adherens was used to explore the effects of PE on junctional proteins. PE-treated monolayers of MDCK cells had markedly decreased immunostaining of ZO-1, a protein of the ZO, but light microscopy of PE-treated cells revealed no obvious morphologic changes. A chromium release assay indicated that, even with marked changes in transmonolayer electrical resistance, the permeability defect was not due to membrane disruption. Fluorescence staining of F-actin indicated diminution of cellular microfilaments in PE-treated cells, but E cadherin (uvomorulin), a protein of the zonula adherens, was unaffected by the enzyme. Elastases from porcine pancreas and human leukocytes with similar enzymatic activity (6 U/ml) did not decrease transmonolayer electrical resistance or degrade ZO-1. These results suggest that PE disturbs the barrier function of epithelial monolayers, in part, by changing the cell architecture and altering at least one protein of the ZO.

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  • In two well-established rodent models of IBS that lead to visceral hyperalgesia, the enhanced visceromotor response to colorectal distension was reversed following administration of F. prausnitzii.

    “Using two well-established rodent models of IBS that lead to visceral hyperalgesia in vivo, it was found that the enhanced visceromotor response to colorectal distension was reversed following administration of F. prausnitzii.”

    From The emerging roles of bacterial proteases in intestinal diseases (2023) · cites this paper as [162]

  • Faecalibacterium prausnitzii is a potential source of anti-nociceptive mediators, including a PAR-4 activating serine protease.

    “Subsequent studies have identified Faecalibacterium prausnitzii as a potential source of anti-nociceptive mediators, including a PAR-4 activating serine protease.”

    From The emerging roles of bacterial proteases in intestinal diseases (2023) · cites this paper as [162]

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  1. Azghani, A. O., L. D. Gray, B. T. Peterson, and A. R. Johnson. 1991. Elastase from Pseudomonas aeruginosa alters integrity of intercellular junctions of cultured epithelial cells. Abstr. 8010. VOL. 61, 1993 FASEB J. 5:A1750.

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Authors
Azghani, A. O., Gray, L. D., & Johnson, A. R.
Affiliations
The University of Texas Health Science Center at Tyler (US)
ORCID
Azghani, A O
Venue
Infection and Immunity
Publisher
American Society for Microbiology
Published
1993-06
Volume
61
Issue
6
Pages
2681-2686
ISSN
  • 0019-9567 (ISSN-L)
  • 1098-5522
DOI
10.1128/iai.61.6.2681-2686.1993
PMC
PMC280900
OpenAlex
W1865065378
Cited by
73
References
35
Citations by year
2026: 2 · 2025: 1 · 2024: 2 · 2023: 3 · 2021: 2 · 2020: 1 · 2019: 3 · 2018: 1 · 2017: 3 · 2015: 4 · 2014: 3 · 2012: 5
FWCI
1.91
Type
journal article
Language
en
Open access
Licence
journals.asm.org/non-commercial-tdm-license (tdm)
Locations
  • Infection and Immunity (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • American Lung Association
  • National Institutes of Health (5r01hl044473-03)
  • National Heart, Lung, and Blood Institute (HL 44473)
Topics
  • Life Sciences › Neuroscience › Neurology › Barrier Structure and Function Studies
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Protease and Inhibitor Mechanisms
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Endocrinology › Escherichia coli research studies
Keywords
epithelial barrier function, tight junctions, ZO-1, zonula occludens, adherens junctions, MDCK cells, paracellular permeability, F-actin, elastase, epithelial permeability
MeSH terms
Animals, Bacterial Proteins, Biological Transport, Cells, Cultured, Cytochalasin B, Dogs, Epithelium, Fluorescent Antibody Technique, Humans, Intercellular Junctions, Metalloendopeptidases, Permeability, Swine, Cadherins

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