Cited by 97Antimicrobial Resistance in StaphylococcusBacterial biofilms and quorum sensingMicrobial infections and disease research

Role of coagulase in a murine model of hematogenous pulmonary infection induced by intravenous injection of Staphylococcus aureus enmeshed in agar beads

Open access
Sawai, T, Tomono, K, Yanagihara, K, Yamamoto, Y, Kaku, M, Hirakata, Y, Koga, H, Tashiro, T +1 more·1997·Infection and Immunity

Abstract

We describe a novel mouse model of acute staphylococcal pneumonia induced by intravenous injection of Staphylococcus aureus enmeshed in agar beads. For comparison, we also used various strains of bacteria, including three strains of S. aureus, two strains of Staphylococcus epidermidis, one strain of Streptococcus pyogenes, three strains of Pseudomonas aeruginosa, and one strain of Klebsiella pneumoniae. All except two strains of S. aureus were cleared rapidly from the lungs. When S. aureus NUMR1 enmeshed in agar beads was injected intravenously, the organisms concentrated and remained in the lung for a period longer than several weeks. Multiple lung abscesses were evident macroscopically, and histological examination of the infected lung showed multiple lung abscesses around the pulmonary arterioles, consisting of bacterial colonies encircled with fibrin filaments and surrounded by inflammatory cells of neutrophils and macrophages. When 14 strains of clinically isolated S. aureus were injected intravenously, the number of bacteria recovered from the lung tissue 7 days after infection correlated with the titer of staphylocoagulase (P < 0.01) but not with the titer of clumping factor. Injection of coagulase-deficient mutant strain DU5843 was associated with a markedly reduced number of viable bacteria isolated from the lung, compared with its coagulase-positive parental strain DU5789. Our results suggest that coagulase may play a role in the development of blood-borne staphylococcal pneumonia in our model. Our animal model is simple and reproducible and resembles blood-borne staphylococcal pneumonia in humans, and it could be useful for investigating the pathogenicity or treatment of staphylococcal pulmonary infection, including infections with methicillin-resistant S. aureus.

Integrity signals

Registry

Crossref · 10.1128/iai.65.2.466-471.1997

Cites

29 of 29 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

The text we found for this paper looks like another document, so its claims and self-check weren’t run. The registry and citation checks still apply.

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The text we found for this paper looks like another document, so the self-check wasn’t run.

Cites

Flagged first, then by citations

Cited by

Sorted by citations

Authors
Sawai, T., Tomono, K., Yanagihara, K., Yamamoto, Y., Kaku, M., Hirakata, Y., Koga, H., Tashiro, T., & Kohno, S.
Affiliations
Nagasaki University (JP)
Venue
Infection and Immunity
Published
1997-02
Volume
65
Issue
2
Pages
466-471
ISSN
  • 0019-9567 (ISSN-L)
  • 1098-5522
OpenAlex
W2116348263
Cited by
97
References
29
Citations by year
2026: 2 · 2025: 4 · 2024: 1 · 2021: 3 · 2020: 2 · 2019: 5 · 2018: 2 · 2016: 5 · 2015: 3 · 2014: 4 · 2013: 6 · 2012: 3
FWCI
1.46
Type
journal article
Language
en
Open access
Locations
  • Infection and Immunity (publishedVersion)
  • Europe PMC (PubMed Central) (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Health Sciences › Medicine › Infectious Diseases › Antimicrobial Resistance in Staphylococcus
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bacterial biofilms and quorum sensing
  • Life Sciences › Immunology and Microbiology › Microbiology › Microbial infections and disease research
Keywords
staphylocoagulase, Staphylococcus aureus, staphylococcal pneumonia, mouse model, lung abscess, clumping factor, bacterial virulence factors, methicillin-resistant Staphylococcus aureus
MeSH terms
Agar, Animals, Coagulase, Disease Models, Animal, Injections, Intravenous, Lung, Male, Mice, Inbred Strains, Microspheres, Pneumonia, Staphylococcal, Staphylococcal Infections, Staphylococcus aureus, Colony Count, Microbial, Bacteremia, Mice

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review