The DOI matches its Crossref record; 12 of the 12 references it cites were found in a registry or an index.
Expression of the Candida albicans Gene ALS1 in Saccharomyces cerevisiae Induces Adherence to Endothelial and Epithelial Cells
Open accessAbstract
To identify genes encoding adhesins that mediate the binding of Candida albicans to endothelial cells, a genomic library from this organism was constructed and used to transform Saccharomyces cerevisiae . These transformed organisms were screened for adherence to endothelial cells, and a highly adherent clone was identified. The adherence of this clone to endothelial cells was over 100-fold greater than that of control S. cerevisiae transformed with the empty plasmid. This clone also exhibited enhanced adherence to epithelial cells. The C. albicans gene contained within this clone was found to be ALS1 . These results indicate that ALS1 may encode a candidal adhesin.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
The source backs part of the claim, not all of it — or backs it for a different population, condition or comparison.
In the citing paper
“even though previous studies have clearly demonstrated that Als1 is an adhesin [36,37].”
From Als3 Is a Candida albicans Invasin That Binds to Cadherins and Induces Endocytosis by Host Cells (2007) · cites this paper as [37]
Experimental — accuracy not yet measured
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Jaffe, E. A., Nachman, R., Becker, C. G., & Minick, C. (1973) · Journal of Clinical Investigation · doi:10.1172/jci107470 · Cited by 7,146
Studies on the transformation of intact yeast cells by the LiAc/SS‐DNA/PEG procedure
Gietz, R. D., Schiestl, R. H., Willems, A. R., & Woods, R. A. (1995) · Yeast · doi:10.1002/yea.320110408 · Cited by 2,030
Elledge, S. J., Mulligan, J. T., Ramer, S. W., Spottswood, M. R., & Davis, R. W. (1991) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.88.5.1731 · Cited by 345
Gaur, N. K., & Klotz, S. A. (1997) · Infection and Immunity · doi:10.1128/iai.65.12.5289-5294.1997 · Cited by 182 · cited 1×
“Recently, Gaur and Klotz (4) described another member of the ALS gene family, ALA1.”
BackgroundLipke, P. N., Wojciechowicz, D. C., & Kurjan, J. (1989) · Molecular and Cellular Biology · doi:10.1128/mcb.9.8.3155-3165.1989 · Cited by 179
Hoyer, L. L., Scherer, S., Shatzman, A. R., & Livi, G. P. (1995) · Molecular Microbiology · doi:10.1111/j.1365-2958.1995.tb02219.x · Cited by 150
Wojciechowicz, D., Lu, C.-F., Kurjan, J., & Lipke, P. (1993) · Molecular and Cellular Biology · doi:10.1128/mcb.13.4.2554-2563.1993 · Cited by 129
Filler, S. G., Pfunder, A. S., Spellberg, B. J., Spellberg, J. P., & Edwards, J. E. (1996) · Infection and Immunity · doi:10.1128/iai.64.7.2609-2617.1996 · Cited by 117
Self-citationWojciechowicz, D. C., Lu, C., Kurjan, J., & Lipke, P. N. (1993) · Molecular and Cellular Biology · doi:10.1128/mcb.13.4.2554 · Cited by 116
Dominant genetics using a yeast genomic library under the control of a strong inducible promoter.
Ramer, S. W., Elledge, S. J., & Davis, R. W. (1992) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.89.23.11589 · Cited by 96
Research works printed but not linked: 1
- Fu Y. S. G. Filler B. J. Spellberg W. A. Fonzi A. S. Ibrahim T. Kanbe M. A. Ghannoum and J. E. Edwards Jr. Cloning and characterization of CAD1/AAF1 a gene from Candida albicans that induces adherence to endothelium when expressed in Saccharomyces cerevisiae . Infect. Immun. in press.
Cited by
Sorted by citations
Items 101–159 of 159
Mao, Y., Zhang, Z., & Wong, B. (2003) · Molecular Microbiology · doi:10.1046/j.1365-2958.2003.03794.x · Cited by 28
Adhesion and Adhesives of Fungi and Oomycetes
Epstein, L., & Nicholson, R. (2006) · Springer eBooks · doi:10.1007/978-3-540-31049-5_3 · Cited by 54
Fungal spore attachment to substrata
Vasselli, J. G., & Shaw, B. D. (2022) · Fungal Biology Reviews · doi:10.1016/j.fbr.2022.03.002 · Cited by 25
Kitanovic, A., NGUYEN, M., VOGL, G., HARTMANN, A., Günther, J., Würzner, R., Künkel, W., Wölfl, S., & Eck, R. (2004) · FEMS Yeast Research · doi:10.1016/j.femsyr.2004.11.005 · Cited by 24
“Interaction with the host cells depends on cell surface proteins mediating adhesion, like the adhesin-like protein Als1p [25], the integrin-like protein Int1p [26] and Hwp1p, a surface protein required for normal hyphal development [27].”
BackgroundOzone ultrafine bubble water inhibits the early formation of Candida albicans biofilms
Shichiri-Negoro, Y., Tsutsumi-Arai, C., Arai, Y., Satomura, K., Arakawa, S., & Wakabayashi, N. (2021) · PLoS ONE · doi:10.1371/journal.pone.0261180 · Cited by 23
“ALS1 helps to enhance adhesion to endothelial cells and encode proteins producing reproductive tracts that grow in host tissues [37].”
BackgroundInteractions of the Fungal Pathogen Candida Albicans with the Host
Rupp, S. (2007) · Future Microbiology · doi:10.2217/17460913.2.2.141 · Cited by 23
“This method identified several adhesins including ALA1/ALS5 [48,49] and ALS1 [50].”
MethodAnandamide prevents the adhesion of filamentous Candida albicans to cervical epithelial cells
Sionov, R. V., Feldman, M., Smoum, R., Mechoulam, R., & Steinberg, D. (2020) · Scientific Reports · doi:10.1038/s41598-020-70650-6 · Cited by 21
Tissue Infection and Site-specific Gene Expression in Candida albicans
Fradin, C., & Hube, B. (2003) · Advances in Applied Microbiology · doi:10.1016/s0065-2164(03)53008-8 · Cited by 21
Between Amyloids and Aggregation Lies a Connection with Strength and Adhesion
Lipke, P., Ramsook, C. B., Garcia-Sherman, M. C., Jackson, D., Chan, C. X. J., Bois, M., & Klotz, S. (2014) · New Journal of Science · doi:10.1155/2014/815102 · Cited by 22
“albicans adhesins [14–16].”
Rast, T. J., Kullas, A. L., Southern, P. J., & Davis, D. A. (2016) · PLoS ONE · doi:10.1371/journal.pone.0153165 · Cited by 22
“cerevisiae as a control, which does not adhere to nor promote epithelial cell damage [24].”
BackgroundHuang, G., Zhang, M., & Erdman, S. E. (2003) · Eukaryotic Cell · doi:10.1128/ec.2.5.1099-1114.2003 · Cited by 21
Mechanisms of Disease and Immunity
Mealey, R. H., & Long, M. T. (2017) · Elsevier eBooks · doi:10.1016/b978-0-323-44329-6.00001-2 · Cited by 16
Adherence of clinical isolates of Saccharomyces cerevisiae to buccal epithelial cells.
Murphy, A., & Kavanagh, K. (2001) · Medical Mycology · doi:10.1080/mmy.39.1.123.127 · Cited by 13
Signal transduction pathways and cell-wall construction in Candida albicans
Navarro-García, F., Eisman, B., Román, E., Nombela, C., & Plá, J. (2001) · Medical Mycology · doi:10.1080/mmy.39.1.87.100 · Cited by 18
Nguyen, H. T., Zhang, R., Inokawa, N., Oura, T., Chen, X., Iwatani, S., Niimi, K., Niimi, M., Holmes, A. R., Cannon, R. D., et al. (2021) · Journal of Oral Microbiology · doi:10.1080/20002297.2021.1879497 · Cited by 13
“albicans adhesion to human epithelial cells, endothelial cells and even abiotic surfaces such as silicone [42,43].”
BackgroundIkeda, K., Inoue, K., Kitagawa, H., Meguro, H., Shimoi, S., & Park, P. (2012) · InTech eBooks · doi:10.5772/30801 · Cited by 12
“In addition to INT1, agglutinin family proteins, ALA1 and ALS1, and the fibronectin binding protein, FLS5, were also involved in blastospore adhesion (Fu et al., 1998; Rauceo et al., 2006).”
BackgroundExpression of Virulence Genes in Candida Albicans
Staib, P., Kretschmar, M., Nichterlein, T., Köhler, G. A., & Morschhäuser, J. (2005) · Kluwer Academic Publishers eBooks · doi:10.1007/0-306-46840-9_23 · Cited by 12
Human fungal pathogen Candida albicans in the postgenomic era: an overview
Kabir, M. A., & Hussain, M. A. (2009) · Expert Review of Anti-infective Therapy · doi:10.1586/14787210.7.1.121 · Cited by 15
Lagree, K., & Mitchell, A. P. (2017) · ASM Press eBooks · doi:10.1128/9781555819583.ch42 · Cited by 9
Mechanisms of Infectious Disease
Reed, S. M., Bayly, W. M., & Sellon, D. C. (2004) · Elsevier eBooks · doi:10.1016/b0-72-169777-1/50004-4 · Cited by 9
Candida albicans Biofilm Development and Its Genetic Control
Desai, J. V., & Mitchell, A. P. (2015) · ASM Press eBooks · doi:10.1128/9781555817466.ch5 · Cited by 8
Hinokitiol Inhibits Candida albicans Adherence to Oral Epithelial Cells
Nakamura, M., Fujibayashi, T., Tominaga, A., Satoh, N., Kawarai, T., Shinozuka, O., Watanabe, H., Yamazaki, T., & Senpuku, H. (2010) · Journal of Oral Biosciences · doi:10.1016/s1349-0079(10)80007-6 · Cited by 7
Younes, S., & Khalaf, R. A. (2013) · Microbiology · doi:10.1099/mic.0.067249-0 · Cited by 7
“Increased S. cerevisiae adherence by transformation of a C. albicans gene has been shown previously for other adhesins such as Als1 (Fu et al., 1998).”
BackgroundManaging Denture Biofilm Related Diseases
Wu, T., Shi, W., Loewy, Z. G., & He, X. (2015) · Dentistry - Open Journal · doi:10.17140/doj-2-116 · Cited by 6
Comparative Genomic Analysis of Pathogenic Yeasts and the Evolution of Virulence
Fitzpatrick, D. A., & Butler, G. (2009) · Springer eBooks · doi:10.1007/978-3-642-03150-2_1 · Cited by 6
Molecular Basis of Fungal Adherence to Endothelial and Epithelial Cells
Filler, S. G., Sheppard, D. C., & Edwards, J. E. (2014) · ASM Press eBooks · doi:10.1128/9781555815776.ch13 · Cited by 5
Self-citationRecent advances in understanding human opportunistic fungal pathogenesis mechanisms
Cramer, R. A., & Perfect, J. R. (2009) · Elsevier eBooks · doi:10.1016/b978-1-4160-5680-5.00002-5 · Cited by 4
Encounters with Mammalian Cells: Survival Strategies ofCandidaSpecies
Vylkova, S., & Lorenz, M. C. (2014) · ASM Press eBooks · doi:10.1128/9781555817176.ch17 · Cited by 5
Virulence mechanisms for fungi, Part II
Perfect, J. R., & Cox, G. M. (2000) · Clinical Microbiology Newsletter · doi:10.1016/s0196-4399(01)80001-5 · Cited by 4
Hameed, A., Hussain, S. A., Ijaz, M. U., & Umer, M. (2020) · Folia Biologica · doi:10.14712/fb2020066030091 · Cited by 3
“A striking feature of Candida albicans biology is its ability to grow as filamentous/ hyphal, pseudohyphal and yeast forms, in which the hyphal/filamentous form is considered to be vital for its pathogenicity as the filamentous form can grow and invade epithelial cells (Fu et al., 1998; Sandovsky-Losica et al., 2006;…”
BackgroundShort Peptides Allowing Preferential Detection of Candida albicans Hyphae
Kaba, H. E. J., Pölderl, A., & Bilitewski, U. (2015) · Analytical Chemistry · doi:10.1021/acs.analchem.5b01156 · Cited by 3
Human Mycoses: The Role of Molecular Biology
Sheppard, D. C., Ibrahim, A. S., & Edwards, J. E. (2004) · Springer eBooks · doi:10.1007/978-1-4419-8859-1_14 · Cited by 3
Self-citationCastaño, I., Peñas, A. D. L., & Cormack, B. (2006) · ASM Press eBooks · doi:10.1128/9781555815776.ch11 · Cited by 5
Increased fungal burden in the gastrointestinal tract of brain-dead organ donors
Nachman, E. J., Ardis, C. K., Ardis, A. K. B., Nieto, J., Bresson, M. M., Robertson, C. M., Seale, M. N., Villafuerte, N. M., Lyu, Z., Preisner, E. C., et al. (2025) · Microbiology Spectrum · doi:10.1128/spectrum.03341-24 · Cited by 2
Molecular and Mechanistic Insights of Yeast Flocculation
Sariki, S. K., Kumawat, R., Singh, R., & Tomar, R. S. (2023) · Springer eBooks · doi:10.1007/978-981-99-2302-1_26 · Cited by 2
Donofrio, F. C. (2007) · Americanae (AECID Library) · Cited by 2
Pilze im Darm — Fakten, Fragen, Forschungsbedarf
Weig, M., Karch, H., & Mühlschlegel, F. A. (1999) · Springer eBooks · doi:10.1007/978-3-642-59963-7_8 · Cited by 2
Activation of oral epithelial EphA2-EFGR signaling by Candida albicans virulence factorsPreprint
Swidergall, M., Solis, N. V., Millet, N., Huang, M. Y., Lin, J., Phan, Q. T., Lazarus, M. D., Wang, Z., Mitchell, A. P., & Filler, S. G. (2018) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/491076 · Cited by 2
Self-citationPostgenomic Approaches to Analyse Candida albicans Pathogenicity
Munro, C. A., Fradin, C., Bader, O., & Hube, B. (2006) · Springer eBooks · doi:10.1007/3-540-30809-1_9 · Cited by 2
Nosanchuk, J., & Deepe, G. (2006) · ASM Press eBooks · doi:10.1128/9781555815776.ch38 · Cited by 1
Comparative Genomics ofCandidaSpecies
Butler, G. (2014) · ASM Press eBooks · doi:10.1128/9781555817176.ch3 · Cited by 1
Persistent colonization of Candida albicans yeast on the tongue in NOD/SCID.e2f1−/− mice
Arai, T., Kinoshita, Y., & Senpuku, H. (2015) · Journal of Infection and Chemotherapy · doi:10.1016/j.jiac.2015.01.001 · Cited by 1
In vitro models of hematogenously disseminated candidiasis
Filler, S. G. (2014) · Virulence · doi:10.4161/viru.27453 · Cited by 1
“albicans ALS gene family mediate endothelial cell adherence.(12-18) Also, C.”
BackgroundSelf-citationIn vitro models to analyse fungal infection
Gola, S., Arana, D. M., Alonso‐Monge, R., Román, E., & Plá, J. (2007) · Springer eBooks · doi:10.1007/978-1-4020-6397-8_2 · Cited by 1
Exploring the fungal wall proteome by mass spectrometry
Yin, Q. Y. (2008) · UvA-DARE (University of Amsterdam) · Cited by 1
Interactions of Fungi with Endothelial Cells
Filler, S. G., & Sheppard, D. C. (2005) · Springer eBooks · doi:10.1007/0-387-25445-5_20
Self-citationSalomon, S., Felk, A., & Schäfer, W. (2004) · Applied mycology and biotechnology · doi:10.1016/s1874-5334(04)80007-8
The Fungal PathogenCandida albicans
Fanning, S., & Mitchell, A. P. (2014) · ASM Press eBooks · doi:10.1128/9781555816636.ch46
Analysis of the CYC1 Promoter in Candida Albicans
Blocker, T. D. (2008)
Pathogenic Development of Candida albicans
Calderone, R., & Herman, D. (2002) · CRC Press eBooks · doi:10.1201/9780203910719.ch18
Vito, E. D. (2007) · Portuguese National Funding Agency for Science, Research and Technology (RCAAP Project by FCT)
Role of Rim101p in the pH response in Candida albicans
Weyler, M. (2007) · HAL
Rosiana, S., Zhang, L.-Y., Kim, G. H. E., Revtovich, A. V., Sukumaran, A., Geddes-McAlister, J., Kirienko, N. V., & Shapiro, R. S. (2020) · bioRxiv · doi:10.1101/2020.10.22.350991
“…biochemical analysis, and have well-described roles in various aspects of adhesion, host-pathogen interactions, filamentation, and fungal virulence (Fu et al. 1998, 2002; Hoyer et al. 1998; Zhao et al. 2004; Sheppard et al. 2004; Ibrahim et al. 2005; Phan et al. 2007; Nobile et al. 2008; Almeida…”
BackgroundOfek, I., Hasty, D. L., & Doyle, R. J. (2014) · ASM Press eBooks · doi:10.1128/9781555817800.ch12
Soumbo, M. (2019) · theses.fr (ABES) · doi:10.70675/39d1c9fbz9275z4211z82fdz3dc792b563f5
“Parmi celles-ci, les adhésines Als1p et Als3p permettent l’adhésion de C. albicans aux cellules endothéliales vasculaires et aux cellules épithéliales buccales (Fu et al., 1998; Zhao et al., 2004).”
Background
Metadata
- Authors
- Fu, Y., Rieg, G., Fonzi, W. A., Belanger, P. H., Edwards, J. E., & Filler, S. G.
- Affiliations
- University of California, Los Angeles (US)
- <!--label omitted: 1-->St. John’s Cardiovascular Research Center, Division of Infectious Diseases, Department of Medicine, Harbor-UCLA Research and Education Institute, Torrance, California 905021;
- Georgetown University (US)
- Venue
- Infection and Immunity
- Publisher
- American Society for Microbiology
- Published
- 1998-04
- Volume
- 66
- Issue
- 4
- Pages
- 1783-1786
- ISSN
- 0019-9567 (ISSN-L)
- 1098-5522
- PMC
- PMC108121
- OpenAlex
- W1965039542
- Cited by
- 159
- References
- 12
- Citations by year
- 2025: 1 · 2023: 1 · 2022: 1 · 2021: 5 · 2020: 3 · 2019: 3 · 2018: 6 · 2017: 3 · 2016: 3 · 2015: 6 · 2014: 10 · 2013: 4 · 2012: 3
- FWCI
- 7.06
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Infection and Immunity (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health
- National Institute of Allergy and Infectious Diseases (P01 AI-37194, R29AI040636, R01AI019990, R01-AI19990, P01AI037194)
- National Center for Research Resources (M01RR000425)
- Topics
- Health Sciences › Medicine › Infectious Diseases › Antifungal resistance and susceptibility
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
- Health Sciences › Medicine › Infectious Diseases › Fungal Infections and Studies
- Keywords
- Candida albicans, adhesins, cell adhesion, endothelial cells, epithelial cells, yeast transformation, genomic library screening, fungal adhesion, host cell binding
- MeSH terms
- Adhesiveness, Candida albicans, Endothelium, Vascular, Epithelial Cells, Fungal Proteins, Genes, Fungal, Humans, Saccharomyces cerevisiae
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
