The DOI matches its Crossref record; 12 of the 12 references it cites were found in a registry or an index.
Expression of the Candida albicans Gene ALS1 in Saccharomyces cerevisiae Induces Adherence to Endothelial and Epithelial Cells
Open accessAbstract
To identify genes encoding adhesins that mediate the binding of Candida albicans to endothelial cells, a genomic library from this organism was constructed and used to transform Saccharomyces cerevisiae . These transformed organisms were screened for adherence to endothelial cells, and a highly adherent clone was identified. The adherence of this clone to endothelial cells was over 100-fold greater than that of control S. cerevisiae transformed with the empty plasmid. This clone also exhibited enhanced adherence to epithelial cells. The C. albicans gene contained within this clone was found to be ALS1 . These results indicate that ALS1 may encode a candidal adhesin.
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“even though previous studies have clearly demonstrated that Als1 is an adhesin [36,37].”
From Als3 Is a Candida albicans Invasin That Binds to Cadherins and Induces Endocytosis by Host Cells (2007) · cites this paper as [37]
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Cites
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Studies on the transformation of intact yeast cells by the LiAc/SS‐DNA/PEG procedure
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Gaur, N. K., & Klotz, S. A. (1997) · Infection and Immunity · doi:10.1128/iai.65.12.5289-5294.1997 · Cited by 182 · cited 1×
“Recently, Gaur and Klotz (4) described another member of the ALS gene family, ALA1.”
BackgroundLipke, P. N., Wojciechowicz, D. C., & Kurjan, J. (1989) · Molecular and Cellular Biology · doi:10.1128/mcb.9.8.3155-3165.1989 · Cited by 179
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Wojciechowicz, D., Lu, C.-F., Kurjan, J., & Lipke, P. (1993) · Molecular and Cellular Biology · doi:10.1128/mcb.13.4.2554-2563.1993 · Cited by 129
Filler, S. G., Pfunder, A. S., Spellberg, B. J., Spellberg, J. P., & Edwards, J. E. (1996) · Infection and Immunity · doi:10.1128/iai.64.7.2609-2617.1996 · Cited by 117
Self-citationWojciechowicz, D. C., Lu, C., Kurjan, J., & Lipke, P. N. (1993) · Molecular and Cellular Biology · doi:10.1128/mcb.13.4.2554 · Cited by 116
Dominant genetics using a yeast genomic library under the control of a strong inducible promoter.
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Research works printed but not linked: 1
- Fu Y. S. G. Filler B. J. Spellberg W. A. Fonzi A. S. Ibrahim T. Kanbe M. A. Ghannoum and J. E. Edwards Jr. Cloning and characterization of CAD1/AAF1 a gene from Candida albicans that induces adherence to endothelium when expressed in Saccharomyces cerevisiae . Infect. Immun. in press.
Cited by
Sorted by citations
Virulence factors of Candida albicans
Calderone, R. A., & Fonzi, W. A. (2001) · Trends in Microbiology · doi:10.1016/s0966-842x(01)02094-7 · Cited by 1,331
“Furthermore, Als1p is essential for virulence in a hematogenously disseminated murine model [Fu et al. (1999) Role of ALS1 in the virulence of Candida albicans, 37th Annual Meeting of the Infectious Disease Society of America, Philadelphia, PA, USA.”
BackgroundSelf-citationAls3 Is a Candida albicans Invasin That Binds to Cadherins and Induces Endocytosis by Host Cells
Phan, Q. T., Myers, C. L., Fu, Y., Sheppard, D. C., Yeaman, M. R., Welch, W. H., Ibrahim, A. S., Edwards, J. E., & Filler, S. G. (2007) · PLoS Biology · doi:10.1371/journal.pbio.0050064 · Cited by 641
“Also, coating beads with rAls1-N resulted in little to no increase in adherence, even though previous studies have clearly demonstrated that Als1 is an adhesin [36,37].”
ResultSelf-citationCandida albicans: A molecular revolution built on lessons from budding yeast
Berman, J., & Sudbery, P. E. (2002) · Nature Reviews Genetics · doi:10.1038/nrg948 · Cited by 578
Regulatory circuitry governing fungal development, drug resistance, and disease.
Shapiro, R. S., Robbins, N., & Cowen, L. E. (2011) · Microbiology and Molecular Biology Reviews · doi:10.1128/mmbr.00045-10 · Cited by 576
“For instance, the expression of Als1 allows binding to epithelial cells (194), while the expression of the Als3 and Hwp1 proteins allows binding to fibrinogen and the salivary pellicle, and the expression of Eap1 proteins allows binding to polystyrene surfaces (420).”
BackgroundThe ALS gene family of Candida albicans.
Hoyer, L. L. (2001) · Trends in Microbiology · doi:10.1016/s0966-842x(01)01984-9 · Cited by 540
Metadata
- Authors
- Fu, Y., Rieg, G., Fonzi, W. A., Belanger, P. H., Edwards, J. E., & Filler, S. G.
- Affiliations
- University of California, Los Angeles (US)
- <!--label omitted: 1-->St. John’s Cardiovascular Research Center, Division of Infectious Diseases, Department of Medicine, Harbor-UCLA Research and Education Institute, Torrance, California 905021;
- Georgetown University (US)
- Venue
- Infection and Immunity
- Publisher
- American Society for Microbiology
- Published
- 1998-04
- Volume
- 66
- Issue
- 4
- Pages
- 1783-1786
- ISSN
- 0019-9567 (ISSN-L)
- 1098-5522
- PMC
- PMC108121
- OpenAlex
- W1965039542
- Cited by
- 159
- References
- 12
- Citations by year
- 2025: 1 · 2023: 1 · 2022: 1 · 2021: 5 · 2020: 3 · 2019: 3 · 2018: 6 · 2017: 3 · 2016: 3 · 2015: 6 · 2014: 10 · 2013: 4 · 2012: 3
- FWCI
- 7.06
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Infection and Immunity (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health
- National Institute of Allergy and Infectious Diseases (P01 AI-37194, R29AI040636, R01AI019990, R01-AI19990, P01AI037194)
- National Center for Research Resources (M01RR000425)
- Topics
- Health Sciences › Medicine › Infectious Diseases › Antifungal resistance and susceptibility
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
- Health Sciences › Medicine › Infectious Diseases › Fungal Infections and Studies
- Keywords
- Candida albicans, adhesins, cell adhesion, endothelial cells, epithelial cells, yeast transformation, genomic library screening, fungal adhesion, host cell binding
- MeSH terms
- Adhesiveness, Candida albicans, Endothelium, Vascular, Epithelial Cells, Fungal Proteins, Genes, Fungal, Humans, Saccharomyces cerevisiae
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