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Cited by 215Legionella and Acanthamoeba researchMicrobial infections and disease researchBacterial Genetics and Biotechnology

The DOI matches its Crossref record; 58 of the 59 references it cites were found in a registry or an index.

Intracellular Growth of Legionella pneumophila in Dictyostelium discoideum, a System for Genetic Analysis of Host-Pathogen Interactions

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Solomon, Jonathan M., Rupper, Adam, Cardelli, James A., Isberg, Ralph R.·2000·Infection and Immunity
DOI 10.1128/iai.68.5.2939-2947.2000PMC PMC97507
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Abstract

Conditions were established in which Legionella pneumophila, an intracellular bacterial pathogen, could replicate within the unicellular organism Dictyostelium discoideum. By several criteria,L. pneumophila grew by the same mechanism within D. discoideum as it does in amoebae and macrophages. Bacteria grew within membrane-bound vesicles associated with rough endoplasmic reticulum, and L. pneumophila dot/icm mutants, blocked for growth in macrophages and amoebae, also did not grow in D. discoideum. Internalized L. pneumophila avoided degradation by D. discoideum and showed evidence of reduced fusion with endocytic compartments. The ability of L. pneumophila to grow within D. discoideum depended on the growth state of the cells.D. discoideum grown as adherent monolayers was susceptible to L. pneumophila infection and to contact-dependent cytotoxicity during high-multiplicity infections, whereas D. discoideum grown in suspension was relatively resistant to cytotoxicity and did not support intracellular growth. Some known D. discoideum mutants were examined for their effect on growth of L. pneumophila. The coronin mutant and the myoA/B double myosin I mutant were more permissive than wild-type strains for intracellular growth. Growth of L. pneumophila in a G β mutant was slightly reduced compared to the parent strain. This work demonstrates the usefulness of the L. pneumophila-D. discoideum system for genetic analysis of host-pathogen interactions.

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Crossref · 10.1128/iai.68.5.2939-2947.2000

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Research works printed but not linked: 2

  1. Mann S. K. O. Devreotes P. N. Elliott S. Jermyn K. Kuspa A. Fechheimer M. Furukawa R. Parent C. A. Segall J. Shaulsky G. Vardy P. H. Williams J. Williams K. L. Firtel R. A. Cell biological, molecular genetic, and biochemical methods used to examine Dictyostelium Cell biology, a laboratory handbook Celis J. E. 1 1998 431 465 Academic Press San Diego, Calif
  2. Uyeda T. Q. P. Titus M. A. Dictyostelium—a model system for cell and developmental biology 1997 43 64 Universal Academy Press Inc. and Yamada Science Foundation Tokyo Japan

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Metadata

Authors
Solomon, J. M., Rupper, A., Cardelli, J. A., & Isberg, R. R.
Affiliations
  • Tufts University (US)
  • Louisiana State University (US)
ORCID
Venue
Infection and Immunity
Publisher
American Society for Microbiology
Published
2000-05
Volume
68
Issue
5
Pages
2939-2947
ISSN
  • 0019-9567 (ISSN-L)
  • 1098-5522
DOI
10.1128/iai.68.5.2939-2947.2000
PMC
PMC97507
OpenAlex
W2104752214
Cited by
215
References
59
Citations by year
2026: 2 · 2025: 5 · 2024: 3 · 2023: 2 · 2022: 1 · 2021: 2 · 2020: 6 · 2019: 4 · 2018: 7 · 2017: 8 · 2016: 2 · 2015: 6 · 2014: 13 · 2013: 15 · 2012: 6
FWCI
7.33
Type
journal article
Language
en
Open access
Licence
journals.asm.org/non-commercial-tdm-license (tdm)
Locations
  • Infection and Immunity (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • University of Minnesota
  • University of Connecticut
  • Johns Hopkins University
  • Medical Research Council
  • National Institute of Diabetes and Digestive and Kidney Diseases (DK-39232)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Endocrinology › Legionella and Acanthamoeba research
  • Life Sciences › Immunology and Microbiology › Microbiology › Microbial infections and disease research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
Keywords
Legionella pneumophila, Dictyostelium discoideum, intracellular bacterial growth, host-pathogen interaction, Dot/Icm system, rough endoplasmic reticulum, membrane vesicles, endocytic compartments, G protein beta subunit
MeSH terms
Animals, Culture Media, Dictyostelium, Genes, Bacterial, Intracellular Fluid, Intracellular Membranes, Lysosomes, Membrane Proteins, Legionella pneumophila

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