In Vitro Resistance of Staphylococcus aureus to Thrombin-Induced Platelet Microbicidal Protein Is Associated with Alterations in Cytoplasmic Membrane Fluidity

Bayer, Arnold S.; Prasad, Rajendra; Chandra, Jyotsna; Koul, Anjni; Smriti, M.; Varma, Archana; Skurray, Ronald A.; Firth, Neville; Brown, Melissa H.; Koo, Su-Pin; Yeaman, Michael R.

doi:10.1128/iai.68.6.3548-3553.2000

Cited by 150Antimicrobial Resistance in StaphylococcusAntimicrobial Peptides and ActivitiesBacterial Genetics and Biotechnology

In Vitro Resistance of Staphylococcus aureus to Thrombin-Induced Platelet Microbicidal Protein Is Associated with Alterations in Cytoplasmic Membrane Fluidity

Open access

Bayer, Arnold S., Prasad, Rajendra, Chandra, Jyotsna, Koul, Anjni, Smriti, M., Varma, Archana, Skurray, Ronald A., Firth, Neville +3 more·2000·Infection and Immunity

DOI 10.1128/iai.68.6.3548-3553.2000PMC PMC97641

Open this paper in CoChat — free account

Abstract

Platelet microbicidal proteins (PMPs) are small, cationic peptides which possess potent microbicidal activities against common bloodstream pathogens, such as Staphylococcus aureus. We previously showed that S. aureus strains exhibiting resistance to thrombin-induced PMP (tPMP-1) in vitro have an enhanced capacity to cause human and experimental endocarditis (T. Wu, M. R. Yeaman, and A. S. Bayer, Antimicrob. Agents Chemother. 38:729-732, 1994; A. S. Bayer et al., Antimicrob. Agents Chemother. 42:3169-3172, 1998; V. K. Dhawan et al., Infect. Immun. 65:3293-3299, 1997). However, the mechanisms mediating tPMP-1 resistance in S. aureus are not fully delineated. The S. aureus cell membrane appears to be a principal target for the action of tPMP-1. To gain insight into the basis of tPMP-1 resistance, we compared several parameters of membrane structure and function in three tPMP-1-resistant (tPMP-1(r)) strains and their genetically related, tPMP-1-susceptible (tPMP-1(s)) counterpart strains. The tPMP-1(r) strains were derived by three distinct methods: transposon mutagenesis, serial passage in the presence of tPMP-1 in vitro, or carriage of a naturally occurring multiresistance plasmid (pSK1). All tPMP-1(r) strains were found to possess elevated levels of longer-chain, unsaturated membrane lipids, in comparison to their tPMP-1(s) counterparts. This was reflected in corresponding differences in cell membrane fluidity in the strain pairs, with tPMP-1(r) strains exhibiting significantly higher degrees of fluidity as assessed by fluorescence polarization. These data provide further support for the concept that specific alterations in the cytoplasmic membrane of S. aureus strains are associated with tPMP-1 resistance in vitro.

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

Integrity signals

Registry

Crossref · 10.1128/iai.68.6.3548-3553.2000

Cites

51 of 51 references verified0 citation errors · 0 not checkable

Statistics

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

The lipid profile of resting cells shows reorganisation after treatment with essential oil compounds.
“The lipid profile of resting cells shows reorganisation after treatment with EO compounds, and many authors have described the bacterial fatty acid synthesis pathway as an optimal target of many antibacterial agents [73,74,75].”
From Effect of essential oils on pathogenic bacteria (2013) · cites this paper as [74]
An increase in the amount of saturated fatty acids in the membrane lipid bilayer results in a loss of membrane fluidity and a consequent increase in membrane rigidity.
“An increase in the amount of SFAs in the membrane lipid bilayer results in a loss of membrane fluidity and a consequent increase in membrane rigidity [74].”
From Effect of essential oils on pathogenic bacteria (2013) · cites this paper as [74]

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Methicillin-resistant Staphylococcus aureus clinical strain with reduced vancomycin susceptibility
Hiramatsu, K. (1997) · Journal of Antimicrobial Chemotherapy · doi:10.1093/jac/40.1.135 · Cited by 1,984 · see citing works
Fluidity parameters of lipid regions determined by fluorescence polarization
Shinitzky, M., & Barenholz, Y. (1978) · Biochimica et Biophysica Acta (BBA) - Reviews on Biomembranes · doi:10.1016/0304-4157(78)90010-2 · Cited by 1,882 · see citing works
The modification of mammalian membrane polyunsaturated fatty acid composition in relation to membrane fluidity and function
Stubbs, C. D., & Smith, A. D. (1984) · Biochimica et Biophysica Acta (BBA) - Reviews on Biomembranes · doi:10.1016/0304-4157(84)90005-4 · Cited by 1,618 · see citing works
Epidemiology of Drug Resistance: Implications for a Post—Antimicrobial Era
Cohen, M. L. (1992) · Science · doi:10.1126/science.257.5073.1050 · Cited by 1,310 · see citing works
The staphylococci in human disease
Crossley, K., & Archer, G. L. (1996) · Churchill Livingstone eBooks · Cited by 625 · see citing works
Molecular cloning and characterization of a novel gene of Candida albicans, CDR1, conferring multiple resistance to drugs and antifungals
Prasad, R., De Wergifosse, P., Goffeau, A., & Balzi, E. (1995) · Current Genetics · doi:10.1007/bf00352101 · Cited by 486 · see citing works
Modulation of Neisseria gonorrhoeae susceptibility to vertebrate antibacterial peptides due to a member of the resistance/nodulation/division efflux pump family
Shafer, W. M., Qu, X. D., Waring, A. J., & Lehrer, R. I. (1998) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.95.4.1829 · Cited by 391 · see citing works
Mode of action of the peptide antibiotic nisin and influence on the membrane potential of whole cells and on cytoplasmic and artificial membrane vesicles
Ruhr, E., & Sahl, H. (1985) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.27.5.841 · Cited by 378 · see citing works
Structure and orientation of the antibiotic peptide magainin in membranes by solid‐state nuclear magnetic resonance spectroscopy
Bechinger, B., ZASLOFF, M. A., & Opella, S. J. (1993) · Protein Science · doi:10.1002/pro.5560021208 · Cited by 360 · see citing works
The Role of Platelets in Antimicrobial Host Defense
Yeaman, M. R. (1997) · Clinical Infectious Diseases · doi:10.1086/516120 · Cited by 308 · see citing works
Membrane Stiffness and Channel Function
Lundbæk, J. A., Birn, P., Girshman, J., Hansen, A. J., & Andersen, O. S. (1996) · Biochemistry · doi:10.1021/bi952250b · Cited by 303 · see citing works
Efflux‐mediated antiseptic resistance gene qacA from Staphylococcus aureus: common ancestry with tetracycline‐ and sugar‐transport proteins
Rouch, D. A., Cram, D. S., DiBerardino, D., Littlejohn, T. G., & Skurray, R. A. (1990) · Molecular Microbiology · doi:10.1111/j.1365-2958.1990.tb00565.x · Cited by 301 · see citing works
Multidrug resistance proteins QacA and QacB from Staphylococcus aureus: membrane topology and identification of residues involved in substrate specificity.
Paulsen, I. T., Brown, M. H., Littlejohn, T. G., Mitchell, B. A., & Skurray, R. A. (1996) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.93.8.3630 · Cited by 291 · see citing works
Defensins
Ganz, T., Selsted, M. E., & Lehrer, R. I. (1990) · European Journal Of Haematology · doi:10.1111/j.1600-0609.1990.tb00339.x · Cited by 249 · see citing works
Cytolytic pore-forming proteins and peptides: is there a common structural motif?
Ojcius, D. M., & Young, J. D. (1991) · Trends in Biochemical Sciences · doi:10.1016/0968-0004(91)90090-i · Cited by 227 · see citing works
Quantitative contributions of cholesterol and the individual classes of phospholipids and their degree of fatty acyl (un)saturation to membrane fluidity measured by fluorescence polarization
BLITTERSWIJK, W. J. V., van der Meer, B. W., & Hilkmann, H. (1987) · Biochemistry · doi:10.1021/bi00380a038 · Cited by 209 · see citing works
The aacA-aphD Gentamicin and Kanamycin Resistance Determinant of Tn4001 from Staphylococcus aureus: Expression and Nucleotide Sequence Analysis
Rouch, D. A., Byrne, M. E., Kong, Y. C., & Skurray, R. A. (1987) · Microbiology · doi:10.1099/00221287-133-11-3039 · Cited by 192 · see citing works
Platelet microbicidal proteins and neutrophil defensin disrupt the Staphylococcus aureus cytoplasmic membrane by distinct mechanisms of action.
Yeaman, M. R., Bayer, A. S., Koo, S.-P., Foss, W. M., & Sullam, P. M. (1998) · Journal of Clinical Investigation · doi:10.1172/jci562 · Cited by 189 · see citing works
Tn4001: A gentamicin and kanamycin resistance transposon in Staphylococcus aureus
Lyon, B. R., May, J. W., & Skurray, R. A. (1984) · Molecular and General Genetics MGG · doi:10.1007/bf00382099 · Cited by 178 · see citing works
Inhibitors of branched-chain amino acid biosynthesis as potential antituberculosis agents
Grandoni, J. A., Marta, P., & Schloss, J. V. (1998) · Journal of Antimicrobial Chemotherapy · doi:10.1093/jac/42.4.475 · Cited by 168 · see citing works
Amino Acid Transport
Cohen, S. R., & Lajtha, Á. (1972) · Springer eBooks · doi:10.1007/978-1-4615-7172-8_21 · Cited by 156 · see citing works
Trimethoprim resistance transposon Tn4003 from Staphylococcus aureus encodes genes for a dihydrofolate reductase and thymidylate synthetase flanked by three copies of IS257
Rouch, D. A., Messerotti, L. J., Loo, L. S., Jackson, C. A., & Skurray, R. A. (1989) · Molecular Microbiology · doi:10.1111/j.1365-2958.1989.tb01805.x · Cited by 151 · see citing works
Dynamic structure of biological membranes as probed by 1,6-diphenyl-1,3,5-hexatriene: a nanosecond fluorescence depolarization study
Kinosita, K., Kataoka, R., Kimura, Y., Gotoh, O., & Ikegami, A. (1981) · Biochemistry · doi:10.1021/bi00518a006 · Cited by 149 · see citing works
Purification and in vitro activities of rabbit platelet microbicidal proteins
Yeaman, M. R., Tang, Y., Shen, A. J., Bayer, A. S., & Selsted, M. E. (1997) · Infection and Immunity · doi:10.1128/iai.65.3.1023-1031.1997 · Cited by 140 · see citing works
Cloning and expression of Staphylococcus aureus plasmid-mediated quaternary ammonium resistance in Escherichia coli
Tennent, J. M., Lyon, B. R., Gillespie, M. T., May, J. W., & Skurray, R. A. (1985) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.27.1.79 · Cited by 130 · see citing works
Partial characterization and staphylocidal activity of thrombin-induced platelet microbicidal protein
Yeaman, M. R., Puentes, S. M., Norman, D. C., & Bayer, A. S. (1992) · Infection and Immunity · doi:10.1128/iai.60.3.1202-1209.1992 · Cited by 128 · see citing works
Fluconazole resistance due to energy-dependent drug efflux in Candida glabrata
Parkinson, T., Falconer, D. J., & Hitchcock, C. A. (1995) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.39.8.1696 · Cited by 127 · see citing works
Nosocomial Bloodstream Infections: Secular Trends in Rates, Mortality, and Contribution to Total Hospital Deaths
Pittet, D. (1995) · Archives of Internal Medicine · doi:10.1001/archinte.155.11.1177 · Cited by 115 · see citing works
QacA Multidrug Efflux Pump from Staphylococcus aureus : Comparative Analysis of Resistance to Diamidines, Biguanidines, and Guanylhydrazones
Mitchell, B. A., Brown, M. H., & Skurray, R. A. (1998) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.42.2.475 · Cited by 113 · see citing works
Analysis of the Staphylococcus epidermidis genes epiF, -E, and -G involved in epidermin immunity
Peschel, A., & Götz, F. (1996) · Journal of Bacteriology · doi:10.1128/jb.178.2.531-536.1996 · Cited by 111 · see citing works
Involvement of bactericidal factors from thrombin-stimulated platelets in clearance of adherent viridans streptococci in experimental infective endocarditis
Dankert, J., van der Werff, J., Zaat, S. A. J., Joldersma, W., Klein, D., & Hess, J. (1995) · Infection and Immunity · doi:10.1128/iai.63.2.663-671.1995 · Cited by 101 · see citing works
Expression of CDR1, a multidrug resistance gene of Candida albicans: transcriptional activation by heat shock, drugs and human steroid hormones
Krishnamurthy, S., Gupta, V., Prasad, R., Panwar, S. L., & Prasad, R. (1998) · FEMS Microbiology Letters · doi:10.1111/j.1574-6968.1998.tb12910.x · Cited by 89 · see citing works
In vitro resistance to platelet microbicidal protein correlates with endocarditis source among bacteremic staphylococcal and streptococcal isolates
Wu, T., Yeaman, M. R., & Bayer, A. S. (1994) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.38.4.729 · Cited by 88 · see citing works
Producer self-protection against the lantibiotic epidermin by the ABC transporter EpiFEG ofStaphylococcus epidermidisTü3298
Otto, M. J., Peschel, A., & GÃ¶tz, F. (1998) · FEMS Microbiology Letters · doi:10.1111/j.1574-6968.1998.tb13891.x · Cited by 85 · see citing works
Plasmid-Mediated Resistance to Thrombin-Induced Platelet Microbicidal Protein in Staphylococci: Role of the qacA Locus
Kupferwasser, L. I., Skurray, R. A., Brown, M. H., Firth, N., Yeaman, M. R., & Bayer, A. S. (1999) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.43.10.2395 · Cited by 85 · see citing works
Staphylocidal action of thrombin-induced platelet microbicidal protein is not solely dependent on transmembrane potential
Koo, S.-P., Bayer, A. S., Sahl, H., Proctor, R. A., & Yeaman, M. R. (1996) · Infection and Immunity · doi:10.1128/iai.64.3.1070-1074.1996 · Cited by 80 · see citing works
Phenotypic resistance to thrombin-induced platelet microbicidal protein in vitro is correlated with enhanced virulence in experimental endocarditis due to Staphylococcus aureus
Dhawan, V. K., Yeaman, M. R., Cheung, A. L., Kim, E.-S., Sullam, P. M., & Bayer, A. S. (1997) · Infection and Immunity · doi:10.1128/iai.65.8.3293-3299.1997 · Cited by 77 · see citing works
Staphylococcus aureus Induces Tissue Factor Expression in Cultured Human Cardiac Valve Endothelium
Drake, T. A., & Pang, M. (1988) · The Journal of Infectious Diseases · doi:10.1093/infdis/157.4.749 · Cited by 76 · see citing works
In Vitro Resistance to Thrombin-Induced Platelet Microbicidal Protein among Clinical Bacteremic Isolates of <i>Staphylococcus aureus</i> Correlates with an Endovascular Infectious Source
Bayer, A. S., Cheng, D., Yeaman, M. R., Corey, G. R., McClelland, R. S., Harrel, L. J., & Fowler, V. G. (1998) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.42.12.3169 · Cited by 73 · see citing works
Nonopsonic phagocytosis of nonmucoid Pseudomonas aeruginosa by human neutrophils and monocyte-derived macrophages is correlated with bacterial piliation and hydrophobicity
Speert, D. P., Loh, B. A., Cabral, D. A., & Salit, I. E. (1986) · Infection and Immunity · doi:10.1128/iai.53.1.207-212.1986 · Cited by 66 · see citing works
Candida albicans: cellular and molecular biology
PRASAD, R. (1993) · Revista do Instituto de Medicina Tropical de São Paulo · doi:10.1590/s0036-46651993000400022 · Cited by 58 · see citing works
In Vitro Resistance to Thrombin-Induced Platelet Microbicidal Protein Is Associated with Enhanced Progression and Hematogenous Dissemination in Experimental Staphylococcus aureus Infective Endocarditis
Dhawan, V. K., Bayer, A. S., & Yeaman, M. R. (1998) · Infection and Immunity · doi:10.1128/iai.66.7.3476-3479.1998 · Cited by 54 · see citing works
Platelet microbicidal protein alone and in combination with antibiotics reduces Staphylococcus aureus adherence to platelets in vitro
Yeaman, M. R., Sullam, P. M., Dazin, P. F., & Bayer, A. S. (1994) · Infection and Immunity · doi:10.1128/iai.62.8.3416-3423.1994 · Cited by 51 · see citing works
Staphylocidal action of thrombin-induced platelet microbicidal protein is influenced by microenvironment and target cell growth phase
Koo, S.-P., Yeaman, M. R., & Bayer, A. S. (1996) · Infection and Immunity · doi:10.1128/iai.64.9.3758-3764.1996 · Cited by 42 · see citing works
Deletion of transmembrane domain 12 ofCDR1, a multidrug transporter fromCandida albicans, leads to altered drug specificity: Expression of a yeast multidrug transporter in baculovirus expression system
Krishnamurthy, S., Chatterjee, U., Gupta, V., Prasad, R., Das, P. K., Snehlata, P., Hasnain, S. E., & Prasad, R. (1998) · Yeast · doi:10.1002/(sici)1097-0061(19980430)14:6 · Cited by 38 · see citing works
The cytoplasmic membrane is a primary target for the staphylocidal action of thrombin-induced platelet microbicidal protein
Koo, S.-P., Yeaman, M. R., Nast, C. C., & Bayer, A. S. (1997) · Infection and Immunity · doi:10.1128/iai.65.11.4795-4800.1997 · Cited by 37 · see citing works
Nutrient transport in Candida albicans, a pathogenic yeast
Prasad, R. (1987) · Yeast · doi:10.1002/yea.320030402 · Cited by 31 · see citing works
Membrane Permeabilization by Thrombin-Induced Platelet Microbicidal Protein 1 Is Modulated by Transmembrane Voltage Polarity and Magnitude
Koo, S.-P., Bayer, A. S., Kagan, B. L., & Yeaman, M. R. (1999) · Infection and Immunity · doi:10.1128/iai.67.5.2475-2481.1999 · Cited by 31 · see citing works
Transport of basic amino acids in Candida albicans
Rao, L. R. S., Prasad, D. S., & Prasad, R. (1986) · Biochimica et Biophysica Acta (BBA) - Biomembranes · doi:10.1016/0005-2736(86)90033-7 · Cited by 18 · see citing works
Alterations in fatty acyl composition can selectively affect amino acid transport in Saccharomyces cerevisiae.
Mishra, P., & Prasad, R. (1987) · PubMed · Cited by 16 · see citing works
Lipids of Candida albicans
Mishra, P., & Prasad, R. (1991) · Springer eBooks · doi:10.1007/978-3-642-75253-7_9 · Cited by 11 · see citing works

Flagged first, then by citations

1 of the 52 references couldn’t be matched to a known paper.

Sorted by citations

Mechanisms of Antimicrobial Peptide Action and Resistance
Yeaman, M. R., & Yount, N. Y. (2003) · Pharmacological Reviews · doi:10.1124/pr.55.1.2 · Cited by 2,998 · see citing works
“Bayer et al. (2000) then compared parameters of membrane structure and function in genetically related S. aureus strains differing in tPMP-1 susceptibility or resistance.”
BackgroundResult
Effect of essential oils on pathogenic bacteria
Nazzaro, F., Fratianni, F., De Martino, L., Coppola, R., & De Feo, V. (2013) · Pharmaceuticals · doi:10.3390/ph6121451 · Cited by 2,186 · see citing works
“An increase in the amount of SFAs in the membrane lipid bilayer results in a loss of membrane fluidity and a consequent increase in membrane rigidity [74].”
Background
Immune evasion by staphylococci
Foster, T. J. (2005) · Nature Reviews Microbiology · doi:10.1038/nrmicro1289 · Cited by 1,214 · see citing works
Mechanisms and consequences of bacterial resistance to antimicrobial peptides
Andersson, D. I., Hughes, D., & Kubicek-Sutherland, J. Z. (2016) · Drug Resistance Updates · doi:10.1016/j.drup.2016.04.002 · Cited by 718 · see citing works
“…membrane fluidity via expression of unsaturated lipids 5 sistan ( 1 7 a l f t m i l t r d T l f b t i s o o a f n d s i 2 r H i g c A a p c b i ( g r 2 s t L m i c i a a e a i i A t i t l 0 D.I. Andersson et al. / Drug Re Bayer et al., 2000) or reducing membrane potential (Yeaman et al., 998).”
Background
Membrane toxicity of antimicrobial compounds from essential oils
Di Pasqua, R., Betts, G., Hoskins, N., Edwards, M., Ercolini, D., & Mauriello, G. (2007) · Journal of Agricultural and Food Chemistry · doi:10.1021/jf0636465 · Cited by 648 · see citing works
Changes in Membrane Fatty Acids Composition of Microbial Cells Induced by Addiction of Thymol, Carvacrol, Limonene, Cinnamaldehyde, and Eugenol in the Growing Media
Pasqua, R. D., Hoskins, N., Betts, G., & Mauriello, G. (2006) · Journal of Agricultural and Food Chemistry · doi:10.1021/jf052722l · Cited by 503 · see citing works
The interaction of bacterial pathogens with platelets
Fitzgerald, J. R., Foster, T. J., & Cox, D. M. (2006) · Nature Reviews Microbiology · doi:10.1038/nrmicro1425 · Cited by 487 · see citing works
How do bacteria resist human antimicrobial peptides?
Peschel, A. (2002) · Trends in Microbiology · doi:10.1016/s0966-842x(02)02333-8 · Cited by 462 · see citing works
Regulation of bacterial drug export systems
Grkovic, S., Brown, M. H., & Skurray, R. A. (2002) · Microbiology and Molecular Biology Reviews · doi:10.1128/mmbr.66.4.671-701.2002 · Cited by 423 · see citing works
“erichia coli (143) and Neisseria gonorrhoeae (203) are also exported by some MDR pumps; in Staphylococcus aureus, the presence of an MDR transporter has been correlated with resistance to a cationic antimicrobial peptide (17, 87).”
Background
Bacterial strategies of resistance to antimicrobial peptides
Joo, H., Fu, C.-I., & Otto, M. J. (2016) · Philosophical Transactions of the Royal Society B Biological Sciences · doi:10.1098/rstb.2015.0292 · Cited by 362 · see citing works
Membrane targeting cationic antimicrobial peptides
Ciumac, D., Gong, H., Hu, X., & Lu, J. R. (2018) · Journal of Colloid and Interface Science · doi:10.1016/j.jcis.2018.10.103 · Cited by 353 · see citing works
Persistent Bacteremia Due to Methicillin‐ResistantStaphylococcus aureusInfection Is Associated withagrDysfunction and Low‐Level In Vitro Resistance to Thrombin‐Induced Platelet Microbicidal Protein
Fowler, V. G., Sakoulas, G., McIntyre, L. M., Meka, V. G., Arbeit, R. D., Cabell, C. H., Stryjewski, M. E., Eliopoulos, G. M., Reller, L. B., Corey, G. R., et al. (2004) · The Journal of Infectious Diseases · doi:10.1086/423145 · Cited by 351 · see citing works
Failures in Clinical Treatment of Staphylococcus aureus Infection with Daptomycin Are Associated with Alterations in Surface Charge, Membrane Phospholipid Asymmetry, and Drug Binding
Jones, T., Yeaman, M. R., Sakoulas, G., Yang, S., Proctor, R. A., Sahl, H., Schrenzel, J., Xiong, Y. Q., & Bayer, A. S. (2007) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.00719-07 · Cited by 320 · see citing works
Mechanisms of daptomycin resistance in <i>Staphylococcus aureus</i>: role of the cell membrane and cell wall
Bayer, A. S., Schneider, T., & Sahl, H. (2013) · Annals of the New York Academy of Sciences · doi:10.1111/j.1749-6632.2012.06819.x · Cited by 311 · see citing works
The Staphylococcus aureus surface protein IsdA mediates resistance to innate defenses of human skin
Clarke, S. R., Mohamed, R., Bian, L., Routh, A. F., Kokai-Kun, J. F., Mond, J. J., Tarkowski, A., & Foster, S. J. (2007) · Cell Host & Microbe · doi:10.1016/j.chom.2007.04.005 · Cited by 225 · see citing works
Pathogenesis of streptococcal and staphylococcal endocarditis
Moreillon, P., Que, Y., & Bayer, A. S. (2002) · Infectious Disease Clinics of North America · doi:10.1016/s0891-5520(01)00009-5 · Cited by 218 · see citing works
“aureus resistant to tPMP-1 studied to date share a common phenotype—a decrease in membrane potential and an increase in membrane fluidity [84,86].”
Background
Analysis of Cell Membrane Characteristics of In Vitro-Selected Daptomycin-Resistant Strains of Methicillin-Resistant Staphylococcus aureus
Mishra, N. N., Yang, S., Sawa, A., Rubio, A., Nast, C. C., Yeaman, M. R., & Bayer, A. S. (2009) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.01682-08 · Cited by 216 · see citing works
Physiological Functions of Bacterial “Multidrug” Efflux Pumps
Henderson, P. J. F., Maher, C., Elbourne, L. D. H., Eijkelkamp, B. A., Paulsen, I. T., & Hassan, K. A. (2021) · Chemical Reviews · doi:10.1021/acs.chemrev.0c01226 · Cited by 203 · see citing works
Carotenoid-Related Alteration of Cell Membrane Fluidity Impacts Staphylococcus aureus Susceptibility to Host Defense Peptides
Mishra, N. N., Liu, G. Y., Yeaman, M. R., Nast, C. C., Proctor, R. A., McKinnell, J. A., & Bayer, A. S. (2010) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.00680-10 · Cited by 204 · see citing works
The possible mechanism of antifungal action of tea tree oil onBotrytis cinerea
Shao, X., Cheng, S., Wang, H., Yu, D., & Mungai, C. (2013) · Journal of Applied Microbiology · doi:10.1111/jam.12193 · Cited by 195 · see citing works
Antimicrobial Peptides – Promising Alternatives to Conventional Antibiotics
Baltzer, S. A., & Brown, M. H. (2011) · Microbial Physiology · doi:10.1159/000331009 · Cited by 191 · see citing works
“However, in addition to the above-mentioned modifications, bacteria can also alter their cell wall hydrophobicity and membrane fluidity, which also plays a role in the protection against APs [Bayer et al., 2000; Peschel, 2002].”
Background
Staphylococcus aureus (Including Staphylococcal Toxic Shock)
Que, Y., & Moreillon, P. (2010) · Elsevier eBooks · doi:10.1016/b978-0-443-06839-3.00195-8 · Cited by 176 · see citing works
Daptomycin Resistance in Enterococci Is Associated with Distinct Alterations of Cell Membrane Phospholipid Content
Mishra, N. N., Bayer, A. S., Tran, T. T., Shamoo, Y. E., Mileykovskaya, E., Dowhan, W., Guan, Z., & Arias, C. A. (2012) · PLoS ONE · doi:10.1371/journal.pone.0043958 · Cited by 168 · see citing works
“The protocol followed previously-published techniques for DPH incorporation into target CMs, measurement of fluorescence polarization and calculation of the degree of fluorescence polarization (polarization index) [46].”
BackgroundMethod
DltABCD- and MprF-Mediated Cell Envelope Modifications of Staphylococcus aureus Confer Resistance to Platelet Microbicidal Proteins and Contribute to Virulence in a Rabbit Endocarditis Model
Weidenmaier, C., Peschel, A., Kempf, V. A. J., Lucindo, N., Yeaman, M. R., & Bayer, A. S. (2005) · Infection and Immunity · doi:10.1128/iai.73.12.8033-8038.2005 · Cited by 166 · see citing works
Staphylococcal multidrug efflux protein QacA.
Brown, M. H., & Skurray, R. A. (2001) · PubMed · Cited by 164 · see citing works
Resistance Mechanisms to Antimicrobial Peptides in Gram-Positive Bacteria
Assoni, L., Milani, B., Carvalho, M. R., Nepomuceno, L. N., Waz, N. T., Guerra, M. E. S., Converso, T. R., & Darrieux, M. (2020) · Frontiers in Microbiology · doi:10.3389/fmicb.2020.593215 · Cited by 166 · see citing works
Reduced Susceptibility of Staphylococcus aureus to Vancomycin and Platelet Microbicidal Protein Correlates with Defective Autolysis and Loss of Accessory Gene Regulator ( agr ) Function
Sakoulas, G., Eliopoulos, G. M., Fowler, V. G., Moellering, R. C., Novick, R. P., Lucindo, N., Yeaman, M. R., & Bayer, A. S. (2005) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.49.7.2687-2692.2005 · Cited by 162 · see citing works
Bacterial resistance mechanisms against host defense peptides
Koprivnjak, T., & Peschel, A. (2011) · Cellular and Molecular Life Sciences · doi:10.1007/s00018-011-0716-4 · Cited by 158 · see citing works
The Staphylococcus aureus Two-Component Regulatory System, GraRS, Senses and Confers Resistance to Selected Cationic Antimicrobial Peptides
Yang, S., Bayer, A. S., Mishra, N. N., Meehl, M. A., Ledala, N., Yeaman, M. R., Xiong, Y. Q., & Cheung, A. L. (2011) · Infection and Immunity · doi:10.1128/iai.05669-11 · Cited by 154 · see citing works
“To assure that any differences seen among the study strains in CAP susceptibility profiles were not attributable to membrane order perturbations, membrane fluidity was determined by fluorescence polarization spectroscopy using the fluorescent probe 1,6-diphenyl-1,3,5hexatriene (DPH) as described previously (4, 29).”
Method
Antimicrobial Peptide Resistance Mechanisms of Gram-Positive Bacteria
Nawrocki, K. L., Crispell, E. K., & McBride, S. M. (2014) · Antibiotics · doi:10.3390/antibiotics3040461 · Cited by 152 · see citing works
“aureus, increased levels of unsaturated membrane lipids increase the resistance to the host AMP, tPMP (thrombin-induced platelet microbicidal proteins) [206].”
Background
Essential Oils: An Impending Substitute of Synthetic Antimicrobial Agents to Overcome Antimicrobial Resistance
Mittal, R. P., Rana, A., & Jaitak, V. (2018) · Current Drug Targets · doi:10.2174/1389450119666181031122917 · Cited by 149 · see citing works
Correlation of Cell Membrane Lipid Profiles with Daptomycin Resistance in Methicillin-Resistant Staphylococcus aureus
Mishra, N. N., & Bayer, A. S. (2012) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.02182-12 · Cited by 137 · see citing works
In Vitro Low-Level Resistance to Azoles in Candida albicans Is Associated with Changes in Membrane Lipid Fluidity and Asymmetry
Kohli, A., Smriti, N., Mukhopadhyay, K., Rattan, A., & Prasad, R. (2002) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.46.4.1046-1052.2002 · Cited by 137 · see citing works
“tance to platelet microbicidal proteins by Staphylococcus aureus was also shown to be associated with variation in membrane fluidity due to elevated levels of unsaturated membrane lipids (4).”
Background
Arming the enemy: the evolution of resistance to self-proteins
Bell, G., & Gouyon, P. (2003) · Microbiology · doi:10.1099/mic.0.26265-0 · Cited by 136 · see citing works
In Vitro Cross-Resistance to Daptomycin and Host Defense Cationic Antimicrobial Peptides in Clinical Methicillin-Resistant Staphylococcus aureus Isolates
Mishra, N. N., McKinnell, J. A., Yeaman, M. R., Rubio, A., Nast, C. C., Chen, L., Kreiswirth, B. N., & Bayer, A. S. (2011) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.00223-11 · Cited by 134 · see citing works
Biocides – resistance, cross-resistance mechanisms and assessment
Gnanadhas, D. P., Marathe, S. A., & Chakravortty, D. (2012) · Expert Opinion on Investigational Drugs · doi:10.1517/13543784.2013.748035 · Cited by 135 · see citing works
“For example, an increase in the amount of unsaturated fatty acids in the membrane increases the fluidity rendering the organism resistant to the antimicrobials [112,113].”
Background
Bacterial Evasion of Host Antimicrobial Peptide Defenses
Cole, J. N., & Nizet, V. (2016) · Microbiology Spectrum · doi:10.1128/microbiolspec.vmbf-0006-2015 · Cited by 126 · see citing works
Insertional Inactivation of Branched-Chain α-Keto Acid Dehydrogenase in Staphylococcus aureus Leads to Decreased Branched-Chain Membrane Fatty Acid Content and Increased Susceptibility to Certain Stresses
Singh, V. K., Hattangady, D. S., Giotis, E. S., Singh, A. K., Chamberlain, N. R., Stuart, M. K., & Wilkinson, B. J. (2008) · Applied and Environmental Microbiology · doi:10.1128/aem.00882-08 · Cited by 127 · see citing works
“aureus to thrombin-induced platelet microbicidal protein is also associated with alterations in cytoplasmic membrane fluidity (4).”
Influential
The intricate link between membrane lipid structure and composition and membrane structural properties in bacterial membranes
Lee, T.-H., Charchar, P., Separovic, F., Reid, G. E., Yarovsky, I., & Aguilar, M. (2024) · Chemical Science · doi:10.1039/d3sc04523d · Cited by 126 · see citing works
Membranes of Class IIa Bacteriocin-Resistant Listeria monocytogenes Cells Contain Increased Levels of Desaturated and Short-Acyl-Chain Phosphatidylglycerols
Vadyvaloo, V., Hastings, J. W., van der Merwe, M. J., & Rautenbach, M. (2002) · Applied and Environmental Microbiology · doi:10.1128/aem.68.11.5223-5230.2002 · Cited by 119 · see citing works
“ionic antimicrobial peptide thrombin-induced platelet microbicidal protein (3).”
Background
Molecular Mechanisms of Bacterial Resistance to Antimicrobial Peptides
Kraus, D., & Peschel, A. (2006) · Springer eBooks · doi:10.1007/3-540-29916-5_9 · Cited by 117 · see citing works
Phenotypic and Genotypic Characteristics of Persistent Methicillin‐ResistantStaphylococcus aureusBacteremia In Vitro and in an Experimental Endocarditis Model
Xiong, Y. Q., Fowler, V. G., Yeaman, M. R., Perdreau‐Remington, F., Kreiswirth, B. N., & Bayer, A. S. (2008) · The Journal of Infectious Diseases · doi:10.1086/595738 · Cited by 115 · see citing works
“Protocols for DPH incorporation into target cell membranes, measurement of fluorescence polarization, and calculation of the degree of fluorescence polarization index are described in detail elsewhere [14].”
BackgroundMethodInfluential
Functional Interrelationships between Cell Membrane and Cell Wall in Antimicrobial Peptide-Mediated Killing of Staphylococcus aureus
Xiong, Y. Q., Mukhopadhyay, K., Yeaman, M. R., Adler‐Moore, J., & Bayer, A. S. (2005) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.49.8.3114-3121.2005 · Cited by 118 · see citing works
“Prior studies have implicated the microbial CM as an initial target for AP-induced killing (1, 25, 31, 49, 52).”
BackgroundMethodInfluential
Fatty Acids Regulate Stress Resistance and Virulence Factor Production for Listeria monocytogenes
Sun, Y., Wilkinson, B. J., Standiford, T. J., Akinbi, H. T., & O’Riordan, M. X. (2012) · Journal of Bacteriology · doi:10.1128/jb.00045-12 · Cited by 116 · see citing works
“Indeed, Staphylococcus aureus strains resistant to thrombin-induced platelet microbicidal proteins, which are CAMPs found in blood, exhibited increased amounts of longer-chain, unsaturated FAs compared to sensitive parental strains (5).”
Background
Efflux Pump Mediated Antimicrobial Resistance by Staphylococci in Health-Related Environments: Challenges and the Quest for Inhibition
Dashtbani-Roozbehani, A., & Brown, M. H. (2021) · Antibiotics · doi:10.3390/antibiotics10121502 · Cited by 118 · see citing works
Serial Daptomycin Selection Generates Daptomycin-NonsusceptibleStaphylococcus aureusStrains with a Heterogeneous Vancomycin-Intermediate Phenotype
Camargo, I. L. B. D. C., Neoh, H., Cui, L., & Hiramatsu, K. (2008) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.00417-08 · Cited by 114 · see citing works
“A total of 30 M DiOC2(3) was added to each sample, and the tubes were incubated at room temperature for 4 min.”
BackgroundMethodInfluential
Addition of Ceftaroline to Daptomycin after Emergence of Daptomycin-Nonsusceptible Staphylococcus aureus during Therapy Improves Antibacterial Activity
Rose, W. E., Schulz, L. T., Andes, D. R., Striker, R., Berti, A. D., Hutson, P. R., & Shukla, S. K. (2012) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.00797-12 · Cited by 112 · see citing works
“The polarization index (pI) is the sensitivity ratio of the instrument for detecting DPH emission and was determined using the following formula: pI [IV IH(IHV/IHH)]/[IV IH(IHV/IHH)],where I is the fluorescence intensity and V and H indicate vertical and horizontal orientation instrument values, respectively (2).”
Method
Staphylococcal resistance to antimicrobial peptides of mammalian and bacterial origin
Peschel, A., & Collins, L. V. (2001) · Peptides · doi:10.1016/s0196-9781(01)00500-9 · Cited by 105 · see citing works
Molecular Mechanisms of Bacterial Resistance to Antimicrobial Peptides in the Modern Era: An Updated Review
Tajer, L., Paillart, J., Dib, H., Sabatier, J., Fajloun, Z., & Khattar, Z. A. (2024) · Microorganisms · doi:10.3390/microorganisms12071259 · Cited by 109 · see citing works
Modification of Fatty Acids in Membranes of Bacteria: Implication for an Adaptive Mechanism to the Toxicity of Carbon Nanotubes
Zhu, B., Xia, X., Xia, N., Zhang, S., & Guo, X. (2014) · Environmental Science & Technology · doi:10.1021/es404359v · Cited by 97 · see citing works
A Mutation in the sap Operon Attenuates Survival of Nontypeable Haemophilus influenzae in a Chinchilla Model of Otitis Media
Mason, K. M., Munson, R. S., & Bakaletz, L. O. (2004) · Infection and Immunity · doi:10.1128/iai.73.1.599-608.2005 · Cited by 95 · see citing works
“Alternative strategies for resisting the toxicity of APs include the efflux of APs via an energy-dependent pump, alterations of the cytoplasmic membrane fluidity, or cleavage with surface-associated proteases (9, 20, 21, 42, 49, 50, 55).”
Background
Frequency and Distribution of Single-Nucleotide Polymorphisms within mprF in Methicillin-Resistant Staphylococcus aureus Clinical Isolates and Their Role in Cross-Resistance to Daptomycin and Host Defense Antimicrobial Peptides
Bayer, A. S., Mishra, N. N., Chen, L., Kreiswirth, B. N., Rubio, A., & Yang, S. (2015) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.00970-15 · Cited by 94 · see citing works
In vitro susceptibility of Staphylococcus aureus to thrombin-induced platelet microbicidal protein-1 (tPMP-1) is influenced by cell membrane phospholipid composition and asymmetry
Mukhopadhyay, K., Whitmire, W. M., Xiong, Y. Q., Molden, J., Jones, T., Peschel, A., Neumann‐Staubitz, P., Adler‐Moore, J., McNamara, P. J., Proctor, R. A., et al. (2007) · Microbiology · doi:10.1099/mic.0.2006/003111-0 · Cited by 92 · see citing works
“Mammalian platelets release a cadre of cationic antimicrobial peptides (CAPs), termed platelet microbicidal proteins (PMPs), which kill many important blood-borne pathogens including Staphylococcus aureus (Bayer et al., 1998, 2000; Wu et al., 1994; Yeaman et al., 1997, 1998).”
Background
Bactericidal Activity of the Human Skin Fatty Acid cis -6-Hexadecanoic Acid on Staphylococcus aureus
Cartron, M. L., England, S. R., Chiriac, A. I., Josten, M., Turner, R. D., Rauter, Y., Hurd, A. F., Sahl, H., Jones, S., & Foster, S. J. (2014) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.01043-13 · Cited by 88 · see citing works
“The lower the polarization index value, the more fluid the membrane (25, 26, 27).”
Background
Defensive remodeling: How bacterial surface properties and biofilm formation promote resistance to antimicrobial peptides
Nuri‬‏, ‪., Shprung, T., & Shai, Y. (2015) · Biochimica et Biophysica Acta (BBA) - Biomembranes · doi:10.1016/j.bbamem.2015.05.022 · Cited by 89 · see citing works
The non‐typeable Haemophilus influenzae Sap transporter provides a mechanism of antimicrobial peptide resistance and SapD‐dependent potassium acquisition
Mason, K. M., Bruggeman, M. E., Munson, R. S., & Bakaletz, L. O. (2006) · Molecular Microbiology · doi:10.1111/j.1365-2958.2006.05460.x · Cited by 82 · see citing works
“…altering cytoplasmic membrane fluidity, cleavage with surface associated proteases (Guo et al., 1998; Shafer et al., 1998; Peschel et al., 1999; Bayer et al., 2000; Guina et al., 2000; McCoy et al., 2001) or reducing the net negative charge of the cell envelope via surface modifications that…”
Background
Bacillus subtilis CF-3 Volatile Organic Compounds Inhibit Monilinia fructicola Growth in Peach Fruit
Zhou, M., Li, P., Wu, S., Zhao, P., & Gao, H. (2019) · Frontiers in Microbiology · doi:10.3389/fmicb.2019.01804 · Cited by 81 · see citing works
“If the saturated fatty acid in the cell membrane is at a high level, it will lead to an increase in the cell membrane stiffness, causing cell rupture (Bayer et al., 2000).”
Background
Volatile organic compounds (VOCs) from Bacillus subtilis CF-3 reduce anthracnose and elicit active defense responses in harvested litchi fruits
Zhao, P., Li, P., Wu, S., Zhou, M., Zhi, R., & Gao, H. (2019) · AMB Express · doi:10.1186/s13568-019-0841-2 · Cited by 81 · see citing works
Cyclic Lipodepsipeptides: A New Class of Antibacterial Agents in the Battle Against Resistant Bacteria
Bionda, N., Pitteloud, J., & Čudić, P. (2013) · Future Medicinal Chemistry · doi:10.4155/fmc.13.86 · Cited by 78 · see citing works
Mucosal physical and chemical innate barriers: Lessons from microbial evasion strategies
Sperandio, B., Fischer, N., & Sansonetti, P. J. (2015) · Seminars in Immunology · doi:10.1016/j.smim.2015.03.011 · Cited by 78 · see citing works
Staphylococcus Aureus Evasion of Innate Antimicrobial Defense
Kraus, D., & Peschel, A. (2008) · Future Microbiology · doi:10.2217/17460913.3.4.437 · Cited by 74 · see citing works
Altering the Proclivity towards Daptomycin Resistance in Methicillin-Resistant Staphylococcus aureus Using Combinations with Other Antibiotics
Berti, A. D., Wergin, J. E., Girdaukas, G. G., Hetzel, S., Sakoulas, G., & Rose, W. E. (2012) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.00502-12 · Cited by 70 · see citing works
“Membrane fluidity was determined spectrofluorometrically using 1,6-diphenyl-1,3,5-hexatriene (DPH) as previously described (3, 5) Overnight bacterial cultures were inoculated into fresh MHB50 medium and grown to an OD600 of 0.”
Method
Cinnamaldehyde Characterization as an Antibacterial Agent toward E. coli Metabolic Profile Using 96-Blade Solid-Phase Microextraction Coupled to Liquid Chromatography–Mass Spectrometry
Mousavi, F., Bojko, B., Bessonneau, V., & Pawliszyn, J. B. (2016) · Journal of Proteome Research · doi:10.1021/acs.jproteome.5b00992 · Cited by 69 · see citing works
Genotypic and Phenotypic Evaluation of the Evolution of High-Level Daptomycin Nonsusceptibility in Vancomycin-Resistant Enterococcus faecium
Humphries, R. M., Kelesidis, T., Tewhey, R., Rose, W. E., Schork, N. J., Nizet, V., & Sakoulas, G. (2012) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.01318-12 · Cited by 68 · see citing works
“d The polarization index is expressed as degree of fluorescence polarization (4).”
BackgroundMethod
Antifungal Mechanism of Volatile Organic Compounds Produced by Bacillus subtilis CF-3 on Colletotrichum gloeosporioides Assessed Using Omics Technology
Wang, K., Qin, Z., Wu, S., Zhao, P., Zhen, C., & Gao, H. (2021) · Journal of Agricultural and Food Chemistry · doi:10.1021/acs.jafc.1c00640 · Cited by 67 · see citing works
Inhibition of Intracellular Macromolecular Synthesis inStaphylococcus aureusby Thrombin‐Induced Platelet Microbicidal Proteins
Xiong, Y.-Q., Bayer, A. S., & Yeaman, M. R. (2002) · The Journal of Infectious Diseases · doi:10.1086/338514 · Cited by 62 · see citing works
“Conceptually, the antimicrobial functions of mammalian platelets have recently been linked to their release of a group of small, cationic peptides termed platelet microbicidal proteins (PMPs) [1,5-17].”
Background
Additional Routes to Staphylococcus aureus Daptomycin Resistance as Revealed by Comparative Genome Sequencing, Transcriptional Profiling, and Phenotypic Studies
Song, Y., Rubio, A., Jayaswal, R. K., Silverman, J. A., & Wilkinson, B. J. (2013) · PLoS ONE · doi:10.1371/journal.pone.0058469 · Cited by 61 · see citing works
Perturbations of Phosphatidate Cytidylyltransferase (CdsA) Mediate Daptomycin Resistance in Streptococcus mitis/oralis by a Novel Mechanism
Mishra, N. N., Tran, T. T., Seepersaud, R., García-de-la-Mària, C., Faull, K. F., Yoon, A., Proctor, R. A., Miró, J. M., Rybak, M. J., Bayer, A. S., et al. (2017) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.02435-16 · Cited by 57 · see citing works
“The protocol for DPH incorporation into target cell membranes, measurement of fluorescence polarization, and calculation of the fluorescence polarization index were described previously (38).”
BackgroundMethod
Reduced Susceptibility to Host-Defense Cationic Peptides and Daptomycin Coemerge in Methicillin-Resistant Staphylococcus aureus From Daptomycin-Naive Bacteremic Patients
Mishra, N. N., Bayer, A. S., Moise, P. A., Yeaman, M. R., & Sakoulas, G. (2012) · The Journal of Infectious Diseases · doi:10.1093/infdis/jis482 · Cited by 56 · see citing works
“adapt to daptomycin exposures in vivo by increasing its membrane fluidity, a key parameter previously associated with resistance to killing by tPMPs [25–27]; by substantially thickening its cell wall, a phenotype that may impede the transit”
Background
Transposon Disruption of the Complex I NADH Oxidoreductase Gene ( snoD ) in Staphylococcus aureus Is Associated with Reduced Susceptibility to the Microbicidal Activity of Thrombin-Induced Platelet Microbicidal Protein 1
Bayer, A. S., McNamara, P. J., Yeaman, M. R., Lucindo, N., Jones, T., Cheung, A. L., Sahl, H., & Proctor, R. A. (2005) · Journal of Bacteriology · doi:10.1128/jb.188.1.211-222.2006 · Cited by 54 · see citing works
Cytoplasmic membrane polarization in Gram-positive and Gram-negative bacteria grown in the absence and presence of tetracycline
Vincent, M., England, L. S., & Trevors, J. T. (2004) · Biochimica et Biophysica Acta (BBA) - General Subjects · doi:10.1016/j.bbagen.2004.03.005 · Cited by 54 · see citing works
Thymol Mediates Bactericidal Activity against Staphylococcus aureus by Targeting an Aldo–Keto Reductase and Consequent Depletion of NADPH
Zhou, W., Wang, Z., Mo, H., Zhao, Y., Li, H., Zhang, H., Hu, L., & Zhou, X. (2019) · Journal of Agricultural and Food Chemistry · doi:10.1021/acs.jafc.9b03517 · Cited by 53 · see citing works
Diversity in Antistaphylococcal Mechanisms among Membrane-Targeting Antimicrobial Peptides
Koo, S.-P., Bayer, A. S., & Yeaman, M. R. (2001) · Infection and Immunity · doi:10.1128/iai.69.8.4916-4922.2001 · Cited by 52 · see citing works
“, small colony variants [SCVs]) or an alteration in their membrane fluidity characteristics (3, 15, 36).”
Background
Low-Level Resistance of Staphylococcus aureus to Thrombin-Induced Platelet Microbicidal Protein 1 In Vitro Associated with qacA Gene Carriage Is Independent of Multidrug Efflux Pump Activity
Bayer, A. S., Kupferwasser, L. I., Brown, M. H., Skurray, R. A., Grkovic, S., Jones, T., Mukhopadhay, K., & Yeaman, M. R. (2006) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.00028-06 · Cited by 51 · see citing works
“aureus strains resist the microbicidal activities of tPMPs remain incompletely defined but appear to include enhanced membrane fluidity (3); reduced transmembrane potential ( ) (17), and mutations of genes involved in generating the proton motive force (PMF) (2).”
BackgroundMethodInfluential
Active Export Proteins Mediating Drug Resistance in Staphylococci
Hassan, K. A., Skurray, R. A., & Brown, M. H. (2007) · Microbial Physiology · doi:10.1159/000099640 · Cited by 51 · see citing works
“Surprisingly, the qacA resistance determinant has also been associated with low-level resistance to the host-encoded thrombin-induced platelet microbicidal protein, which appears to occur via a nonefflux-related mechanism [Bayer et al., 2000, 2006; Kupferwasser et al., 1999].”
Background
Growth and membrane polarization in Pseudomonas aeruginosa UG2 grown in randomized microgravity in a high aspect ratio vessel
England, L. S., Gorzelak, M., & Trevors, J. T. (2003) · Biochimica et Biophysica Acta (BBA) - General Subjects · doi:10.1016/j.bbagen.2003.09.012 · Cited by 49 · see citing works
Role of the LytSR Two-Component Regulatory System in Adaptation to Cationic Antimicrobial Peptides in Staphylococcus aureus
Yang, S., Xiong, Y. Q., Yeaman, M. R., Bayles, K. W., Abdelhady, W., & Bayer, A. S. (2013) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.00412-13 · Cited by 46 · see citing works
Induced resistance to the antimicrobial peptide lactoferricin B in Staphylococcus aureus
Samuelsen, Ø., Haukland, H. H., Jenssen, H., Kramer, M. M., Sandvik, K., Ulvatne, H., & Vorland, L. H. (2005) · FEBS Letters · doi:10.1016/j.febslet.2005.05.017 · Cited by 46 · see citing works
“brane [12,13] would prevent the peptide from reaching its target.”
Site-Specific Mutation of the Sensor Kinase GraS in Staphylococcus aureus Alters the Adaptive Response to Distinct Cationic Antimicrobial Peptides
Cheung, A. L., Bayer, A. S., Yeaman, M. R., Xiong, Y. Q., Waring, A. J., Memmi, G., Donegan, N. P., Chaili, S., & Yang, S. (2014) · Infection and Immunity · doi:10.1128/iai.02480-14 · Cited by 45 · see citing works
“troscopy using the fluorescent probe 1,6-diphenyl-1,3,5-hexatriene (DPH) as described before (11, 40).”
Method
Staphylococcus aureus (Including Staphylococcal Toxic Shock Syndrome)
Que, Y., & Moreillon, P. (2015) · Elsevier eBooks · doi:10.1016/b978-1-4557-4801-3.00196-x · Cited by 43 · see citing works
A current opinion on the antimicrobial importance of popular pepper essential oil and its application in food industry
Myszka, K., Leja, K., & Majcher, M. A. (2018) · Journal of Essential Oil Research · doi:10.1080/10412905.2018.1511482 · Cited by 42 · see citing works
Roles of pyruvate dehydrogenase and branched-chain α-keto acid dehydrogenase in branched-chain membrane fatty acid levels and associated functions in Staphylococcus aureus
Singh, V. K., Sirobhushanam, S., Ring, R. P., Singh, S., Gatto, C., & Wilkinson, B. J. (2018) · Journal of Medical Microbiology · doi:10.1099/jmm.0.000707 · Cited by 42 · see citing works
“aureus strain JE2 and its derivative mutants and complemented strains Membrane fluidity was determined using fluorescence polarization as previously described [38], with some modifications.”
Method
Differential Adaptive Responses of Staphylococcus aureus to In Vitro Selection with Different Antimicrobial Peptides
Shireen, T., Singh, M., Das, T., & Mukhopadhyay, K. (2013) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.00780-13 · Cited by 40 · see citing works
Inhibitory effect and underlying mechanism of cinnamon and clove essential oils on Botryosphaeria dothidea and Colletotrichum gloeosporioides causing rots in postharvest bagging-free apple fruits
Wang, D., Wang, G., Wang, J., Zhai, H., & Xue, X. (2023) · Frontiers in Microbiology · doi:10.3389/fmicb.2023.1109028 · Cited by 38 · see citing works
Contribution of Cell Surface Hydrophobicity in the Resistance ofStaphylococcus aureusagainst Antimicrobial Agents
Lather, P., Mohanty, A. K., Jha, P. K., & Garsa, A. K. (2016) · Biochemistry Research International · doi:10.1155/2016/1091290 · Cited by 38 · see citing works
“aureus also acquired resistance to platelet microbial protein, a small cationic peptide that possesses potentmicrobicidal activities against common blood streampathogens [26].”
Background
The contribution of leucocytes to the antimicrobial activity of platelet-rich plasma preparations: A systematic review
D’Asta, F., Halstead, F. D., Harrison, P., Orlandini, S. Z., Moiemen, N. S., & Lord, J. M. (2017) · Platelets · doi:10.1080/09537104.2017.1317731 · Cited by 37 · see citing works
“different bacterial strains have dissimilar susceptibility to inhibition by platelet AMPs [38].”
BackgroundInfluential
Factors Determining Staphylococcus aureus Susceptibility to Photoantimicrobial Chemotherapy: RsbU Activity, Staphyloxanthin Level, and Membrane Fluidity
Kossakowska-Zwierucho, M., Kaźmierkiewicz, R., Bielawski, K. P., & Nakonieczna, J. (2016) · Frontiers in Microbiology · doi:10.3389/fmicb.2016.01141 · Cited by 34 · see citing works
Structure and Thermotropic Behavior of the Staphylococcus aureus Lipid Lysyl-Dipalmitoylphosphatidylglycerol
Danner, S., Pabst, G., Lohner, K., & Hickel, A. (2007) · Biophysical Journal · doi:10.1529/biophysj.107.123422 · Cited by 34 · see citing works
“aureus resistance to these peptides (19,21,23,31,32).”
Fatty acid composition modulates sensitivity of Legionella pneumophila to warnericin RK, an antimicrobial peptide
Verdon, J., Labanowski, J., Sahr, T., Ferreira, T., Lacombe, C., Buchrieser, C., Berjeaud, J., & Héchard, Y. (2010) · Biochimica et Biophysica Acta (BBA) - Biomembranes · doi:10.1016/j.bbamem.2010.12.011 · Cited by 33 · see citing works
Phenotypic and genotypic correlates of daptomycin-resistant methicillin-susceptible Staphylococcus aureus clinical isolates
Kang, K.-M., Mishra, N. N., Park, K. T., Lee, G. Y., Park, Y. H., Bayer, A. S., & Yang, S. (2017) · The Journal of Microbiology · doi:10.1007/s12275-017-6509-1 · Cited by 30 · see citing works
“The comparative CM fluidity of the three MSSA strain-pairs were determined by fluorescence polarization spectroscopy using the fluorescent probe 1,6-diphenyl-1,3,5-hexatriene (DPH) as described previously (Bayer et al., 2000; Mishra et al., 2009).”
Method
Effect of phosphatidylcholine bilayer thickness and molecular order on the binding of the antimicrobial peptide maculatin 1.1
Lee, T.-H., Sani, M., Overall, S. A., Separovic, F., & Aguilar, M. (2017) · Biochimica et Biophysica Acta (BBA) - Biomembranes · doi:10.1016/j.bbamem.2017.10.007 · Cited by 30 · see citing works
Combination Antibiotic Exposure Selectively Alters the Development of Vancomycin Intermediate Resistance in Staphylococcus aureus
Zheng, X., Berti, A. D., McCrone, S., Roch, M., Rosato, A. E., Rose, W. E., & Chen, B. (2017) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.02100-17 · Cited by 29 · see citing works
“The polarization index (p) was determined using the formula p (IV IH)/(IV IH), where I is the fluorescence intensity, and subscripts V and H indicate vertical and horizontal orientation instrument values, respectively (52).”
Method
Dioxin impacts on lipid metabolism of soil microbes: towards effective detection and bioassessment strategies
Mahfouz, S., Mansour, G., Murphy, D. J., & Hanano, A. (2020) · Bioresources and Bioprocessing · doi:10.1186/s40643-020-00347-1 · Cited by 29 · see citing works
Imaging mass spectrometry reveals complex lipid distributions across Staphylococcus aureus biofilm layers
Rivera, E. S., Weiss, A., Migas, L. G., Freiberg, J. A., Djambazova, K. V., Neumann, E. K., de Plas, R. V., Spraggins, J. M., Skaar, E. P., & Caprioli, R. M. (2022) · Journal of Mass Spectrometry and Advances in the Clinical Lab · doi:10.1016/j.jmsacl.2022.09.003 · Cited by 28 · see citing works
Characterization of cell membrane parameters of clinical isolates of Staphylococcus aureus with varied susceptibility to alpha-melanocyte stimulating hormone
Shireen, T., Singh, M., Dhawan, B., & Mukhopadhyay, K. (2012) · Peptides · doi:10.1016/j.peptides.2012.05.025 · Cited by 28 · see citing works
Characterization of genetic changes associated with daptomycin nonsusceptibility in Staphylococcus aureus
Ma, Z., Lasek‐Nesselquist, E., Lu, J., Schneider, R. F., Shah, R., Oliva, G., Pata, J. D., McDonough, K. A., Pai, M. P., Rose, W. E., et al. (2018) · PLoS ONE · doi:10.1371/journal.pone.0198366 · Cited by 26 · see citing works
A comprehensive guide on screening and selection of a suitable AMP against biofilm-forming bacteria
Anand, A. A., Amod, A., Anwar, S., Sahoo, A. K., Sethi, G., & Samanta, S. K. (2023) · Critical Reviews in Microbiology · doi:10.1080/1040841x.2023.2293019 · Cited by 24 · see citing works
Nontraditional therapies to treat Helicobacter pylori infection
Makobongo, M. O., Gilbreath, J. J., & Merrell, D. S. (2014) · The Journal of Microbiology · doi:10.1007/s12275-014-3603-5 · Cited by 22 · see citing works
Phenotypic and Genotypic Characteristics of Methicillin-Resistant Staphylococcus aureus (MRSA) Related to Persistent Endovascular Infection
Li, L., Yeaman, M. R., Bayer, A. S., & Xiong, Y. Q. (2019) · Antibiotics · doi:10.3390/antibiotics8020071 · Cited by 21 · see citing works
“Additionally, it is known that bacterial membrane fluidity can affect PMP susceptibility [28].”
Background
Bacterial Evasion of Host Antimicrobial Peptide Defenses
Cole, J. N., & Nizet, V. (2016) · ASM Press eBooks · doi:10.1128/9781555819286.ch15 · Cited by 21 · see citing works

In vitro susceptibility of Staphylococcus aureus to thrombin-induced platelet microbicidal protein-1 (tPMP-1) is influenced by cell membrane phospholipid composition and asymmetry
Mukhopadhyay, K., Whitmire, W. M., Xiong, Y. Q., Molden, J., Jones, T., Peschel, A., Neumann‐Staubitz, P., Adler‐Moore, J., McNamara, P. J., Proctor, R. A., et al. (2007) · Microbiology · doi:10.1099/mic.0.2006/003111-0 · Cited by 92 · see citing works
Correspondence
Bayer, A. S. (2006)
The cytoplasmic membrane is a primary target for the staphylocidal action of thrombin-induced platelet microbicidal protein
Koo, S.-P., Yeaman, M. R., Nast, C. C., & Bayer, A. S. (1997) · Infection and Immunity · doi:10.1128/iai.65.11.4795-4800.1997 · Cited by 37 · see citing works
Phenotypic resistance to thrombin-induced platelet microbicidal protein in vitro is correlated with enhanced virulence in experimental endocarditis due to Staphylococcus aureus
Dhawan, V. K., Yeaman, M. R., Cheung, A. L., Kim, E.-S., Sullam, P. M., & Bayer, A. S. (1997) · Infection and Immunity · doi:10.1128/iai.65.8.3293-3299.1997 · Cited by 77 · see citing works
Low-Level Resistance of Staphylococcus aureus to Thrombin-Induced Platelet Microbicidal Protein 1 In Vitro Associated with qacA Gene Carriage Is Independent of Multidrug Efflux Pump Activity
Bayer, A. S., Kupferwasser, L. I., Brown, M. H., Skurray, R. A., Grkovic, S., Jones, T., Mukhopadhay, K., & Yeaman, M. R. (2006) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.00028-06 · Cited by 51 · see citing works

Authors: Bayer, A. S., Prasad, R., Chandra, J., Koul, A., Smriti, M., Varma, A., Skurray, R. A., Firth, N., Brown, M. H., Koo, S.-P., & Yeaman, M. R.
Affiliations: University of California, Los Angeles (US)
Jawaharlal Nehru University (IN)
The University of Sydney (AU)
ORCID: Bayer, Arnold S.
Prasad, Rajendra
Chandra, Jyotsna
Koul, Anjni
Firth, Neville
Brown, Melissa H.
Yeaman, Michael R.
Venue: Infection and Immunity
Publisher: American Society for Microbiology
Published: 2000-06
Volume: 68
Issue: 6
Pages: 3548-3553
ISSN: 0019-9567 (ISSN-L)
1098-5522
DOI: 10.1128/iai.68.6.3548-3553.2000
PMC: PMC97641
OpenAlex: W2156172406
Cited by: 150
References: 52
Citations by year: 2026: 1 · 2025: 4 · 2024: 4 · 2023: 4 · 2022: 2 · 2021: 8 · 2020: 5 · 2019: 5 · 2018: 7 · 2017: 7 · 2016: 11 · 2015: 7 · 2014: 6 · 2013: 9 · 2012: 14
FWCI: 3.64
Type: journal article
Language: en
Open access: green
www.ncbi.nlm.nih.gov
Licence: journals.asm.org/non-commercial-tdm-license (tdm)
Locations: Infection and Immunity (publishedVersion)
PubMed Central (publishedVersion)
PubMed (publishedVersion)
Funders: National Institutes of Health
National Institute of Allergy and Infectious Diseases (AI 39108, AI-39001, R01AI039108, R29AI039001, R01AI039001)
Topics: Health Sciences › Medicine › Infectious Diseases › Antimicrobial Resistance in Staphylococcus
Life Sciences › Immunology and Microbiology › Microbiology › Antimicrobial Peptides and Activities
Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
Keywords: Staphylococcus aureus, antimicrobial resistance, membrane lipid composition, endocarditis, cationic antimicrobial peptides, multiresistance plasmid
MeSH terms: Amino Acids, Anti-Bacterial Agents, beta-Thromboglobulin, Biological Transport, Blood Proteins, Cell Membrane, DNA Transposable Elements, Drug Resistance, Microbial, Fatty Acids, Membrane Fluidity, Phenotype, Phospholipids, Species Specificity, Staphylococcus aureus, Mutagenesis, Insertional, Chemokines

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review

In Vitro Resistance of Staphylococcus aureus to Thrombin-Induced Platelet Microbicidal Protein Is Associated with Alterations in Cytoplasmic Membrane Fluidity

Abstract

Integrity signals

Claims the paper makes

Claims other papers make about this one

Self-check

Cites

Cited by

Related

Record

About these checks