The DOI matches its Crossref record; 51 of the 52 references it cites were found in a registry or an index.
In Vitro Resistance of Staphylococcus aureus to Thrombin-Induced Platelet Microbicidal Protein Is Associated with Alterations in Cytoplasmic Membrane Fluidity
Open accessAbstract
Platelet microbicidal proteins (PMPs) are small, cationic peptides which possess potent microbicidal activities against common bloodstream pathogens, such as Staphylococcus aureus. We previously showed that S. aureus strains exhibiting resistance to thrombin-induced PMP (tPMP-1) in vitro have an enhanced capacity to cause human and experimental endocarditis (T. Wu, M. R. Yeaman, and A. S. Bayer, Antimicrob. Agents Chemother. 38:729-732, 1994; A. S. Bayer et al., Antimicrob. Agents Chemother. 42:3169-3172, 1998; V. K. Dhawan et al., Infect. Immun. 65:3293-3299, 1997). However, the mechanisms mediating tPMP-1 resistance in S. aureus are not fully delineated. The S. aureus cell membrane appears to be a principal target for the action of tPMP-1. To gain insight into the basis of tPMP-1 resistance, we compared several parameters of membrane structure and function in three tPMP-1-resistant (tPMP-1(r)) strains and their genetically related, tPMP-1-susceptible (tPMP-1(s)) counterpart strains. The tPMP-1(r) strains were derived by three distinct methods: transposon mutagenesis, serial passage in the presence of tPMP-1 in vitro, or carriage of a naturally occurring multiresistance plasmid (pSK1). All tPMP-1(r) strains were found to possess elevated levels of longer-chain, unsaturated membrane lipids, in comparison to their tPMP-1(s) counterparts. This was reflected in corresponding differences in cell membrane fluidity in the strain pairs, with tPMP-1(r) strains exhibiting significantly higher degrees of fluidity as assessed by fluorescence polarization. These data provide further support for the concept that specific alterations in the cytoplasmic membrane of S. aureus strains are associated with tPMP-1 resistance in vitro.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
In the citing paper
“The lipid profile of resting cells shows reorganisation after treatment with EO compounds, and many authors have described the bacterial fatty acid synthesis pathway as an optimal target of many antibacterial agents [73,74,75].”
From Effect of essential oils on pathogenic bacteria (2013) · cites this paper as [74]
In the citing paper
“An increase in the amount of SFAs in the membrane lipid bilayer results in a loss of membrane fluidity and a consequent increase in membrane rigidity [74].”
From Effect of essential oils on pathogenic bacteria (2013) · cites this paper as [74]
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Methicillin-resistant Staphylococcus aureus clinical strain with reduced vancomycin susceptibility
Hiramatsu, K. (1997) · Journal of Antimicrobial Chemotherapy · doi:10.1093/jac/40.1.135 · Cited by 1,984
Fluidity parameters of lipid regions determined by fluorescence polarization
Shinitzky, M., & Barenholz, Y. (1978) · Biochimica et Biophysica Acta (BBA) - Reviews on Biomembranes · doi:10.1016/0304-4157(78)90010-2 · Cited by 1,885
Stubbs, C. D., & Smith, A. D. (1984) · Biochimica et Biophysica Acta (BBA) - Reviews on Biomembranes · doi:10.1016/0304-4157(84)90005-4 · Cited by 1,618
Epidemiology of Drug Resistance: Implications for a Post—Antimicrobial Era
Cohen, M. L. (1992) · Science · doi:10.1126/science.257.5073.1050 · Cited by 1,310
The staphylococci in human disease
Crossley, K., & Archer, G. L. (1996) · Churchill Livingstone eBooks · Cited by 625
Prasad, R., De Wergifosse, P., Goffeau, A., & Balzi, E. (1995) · Current Genetics · doi:10.1007/bf00352101 · Cited by 486
Shafer, W. M., Qu, X. D., Waring, A. J., & Lehrer, R. I. (1998) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.95.4.1829 · Cited by 391
Ruhr, E., & Sahl, H. (1985) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.27.5.841 · Cited by 377
Bechinger, B., ZASLOFF, M. A., & Opella, S. J. (1993) · Protein Science · doi:10.1002/pro.5560021208 · Cited by 360
The Role of Platelets in Antimicrobial Host Defense
Yeaman, M. R. (1997) · Clinical Infectious Diseases · doi:10.1086/516120 · Cited by 308
Membrane Stiffness and Channel Function
Lundbæk, J. A., Birn, P., Girshman, J., Hansen, A. J., & Andersen, O. S. (1996) · Biochemistry · doi:10.1021/bi952250b · Cited by 304
Rouch, D. A., Cram, D. S., DiBerardino, D., Littlejohn, T. G., & Skurray, R. A. (1990) · Molecular Microbiology · doi:10.1111/j.1365-2958.1990.tb00565.x · Cited by 301
Paulsen, I. T., Brown, M. H., Littlejohn, T. G., Mitchell, B. A., & Skurray, R. A. (1996) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.93.8.3630 · Cited by 291
Ganz, T., Selsted, M. E., & Lehrer, R. I. (1990) · European Journal Of Haematology · doi:10.1111/j.1600-0609.1990.tb00339.x · Cited by 249
Cytolytic pore-forming proteins and peptides: is there a common structural motif?
Ojcius, D. M., & Young, J. D. (1991) · Trends in Biochemical Sciences · doi:10.1016/0968-0004(91)90090-i · Cited by 227
BLITTERSWIJK, W. J. V., van der Meer, B. W., & Hilkmann, H. (1987) · Biochemistry · doi:10.1021/bi00380a038 · Cited by 209
Rouch, D. A., Byrne, M. E., Kong, Y. C., & Skurray, R. A. (1987) · Microbiology · doi:10.1099/00221287-133-11-3039 · Cited by 192
Yeaman, M. R., Bayer, A. S., Koo, S.-P., Foss, W. M., & Sullam, P. M. (1998) · Journal of Clinical Investigation · doi:10.1172/jci562 · Cited by 189
Tn4001: A gentamicin and kanamycin resistance transposon in Staphylococcus aureus
Lyon, B. R., May, J. W., & Skurray, R. A. (1984) · Molecular and General Genetics MGG · doi:10.1007/bf00382099 · Cited by 178
Inhibitors of branched-chain amino acid biosynthesis as potential antituberculosis agents
Grandoni, J. A., Marta, P., & Schloss, J. V. (1998) · Journal of Antimicrobial Chemotherapy · doi:10.1093/jac/42.4.475 · Cited by 168
Cohen, S. R., & Lajtha, Á. (1972) · Springer eBooks · doi:10.1007/978-1-4615-7172-8_21 · Cited by 156
Rouch, D. A., Messerotti, L. J., Loo, L. S., Jackson, C. A., & Skurray, R. A. (1989) · Molecular Microbiology · doi:10.1111/j.1365-2958.1989.tb01805.x · Cited by 151
Kinosita, K., Kataoka, R., Kimura, Y., Gotoh, O., & Ikegami, A. (1981) · Biochemistry · doi:10.1021/bi00518a006 · Cited by 149
Purification and in vitro activities of rabbit platelet microbicidal proteins
Yeaman, M. R., Tang, Y., Shen, A. J., Bayer, A. S., & Selsted, M. E. (1997) · Infection and Immunity · doi:10.1128/iai.65.3.1023-1031.1997 · Cited by 140
Tennent, J. M., Lyon, B. R., Gillespie, M. T., May, J. W., & Skurray, R. A. (1985) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.27.1.79 · Cited by 130
Fluconazole resistance due to energy-dependent drug efflux in Candida glabrata
Parkinson, T., Falconer, D. J., & Hitchcock, C. A. (1995) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.39.8.1696 · Cited by 129
Yeaman, M. R., Puentes, S. M., Norman, D. C., & Bayer, A. S. (1992) · Infection and Immunity · doi:10.1128/iai.60.3.1202-1209.1992 · Cited by 128
Pittet, D. (1995) · Archives of Internal Medicine · doi:10.1001/archinte.155.11.1177 · Cited by 115
Mitchell, B. A., Brown, M. H., & Skurray, R. A. (1998) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.42.2.475 · Cited by 113
Analysis of the Staphylococcus epidermidis genes epiF, -E, and -G involved in epidermin immunity
Peschel, A., & Götz, F. (1996) · Journal of Bacteriology · doi:10.1128/jb.178.2.531-536.1996 · Cited by 110
Dankert, J., van der Werff, J., Zaat, S. A. J., Joldersma, W., Klein, D., & Hess, J. (1995) · Infection and Immunity · doi:10.1128/iai.63.2.663-671.1995 · Cited by 101
Krishnamurthy, S., Gupta, V., Prasad, R., Panwar, S. L., & Prasad, R. (1998) · FEMS Microbiology Letters · doi:10.1111/j.1574-6968.1998.tb12910.x · Cited by 89
Wu, T., Yeaman, M. R., & Bayer, A. S. (1994) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.38.4.729 · Cited by 88
Otto, M. J., Peschel, A., & Götz, F. (1998) · FEMS Microbiology Letters · doi:10.1111/j.1574-6968.1998.tb13891.x · Cited by 87
Kupferwasser, L. I., Skurray, R. A., Brown, M. H., Firth, N., Yeaman, M. R., & Bayer, A. S. (1999) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.43.10.2395 · Cited by 85
Koo, S.-P., Bayer, A. S., Sahl, H., Proctor, R. A., & Yeaman, M. R. (1996) · Infection and Immunity · doi:10.1128/iai.64.3.1070-1074.1996 · Cited by 80
Dhawan, V. K., Yeaman, M. R., Cheung, A. L., Kim, E.-S., Sullam, P. M., & Bayer, A. S. (1997) · Infection and Immunity · doi:10.1128/iai.65.8.3293-3299.1997 · Cited by 77
Staphylococcus aureus Induces Tissue Factor Expression in Cultured Human Cardiac Valve Endothelium
Drake, T. A., & Pang, M. (1988) · The Journal of Infectious Diseases · doi:10.1093/infdis/157.4.749 · Cited by 76
Bayer, A. S., Cheng, D., Yeaman, M. R., Corey, G. R., McClelland, R. S., Harrel, L. J., & Fowler, V. G. (1998) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.42.12.3169 · Cited by 73
Speert, D. P., Loh, B. A., Cabral, D. A., & Salit, I. E. (1986) · Infection and Immunity · doi:10.1128/iai.53.1.207-212.1986 · Cited by 66
Candida albicans: cellular and molecular biology
PRASAD, R. (1993) · Revista do Instituto de Medicina Tropical de São Paulo · doi:10.1590/s0036-46651993000400022 · Cited by 58
Dhawan, V. K., Bayer, A. S., & Yeaman, M. R. (1998) · Infection and Immunity · doi:10.1128/iai.66.7.3476-3479.1998 · Cited by 54
Yeaman, M. R., Sullam, P. M., Dazin, P. F., & Bayer, A. S. (1994) · Infection and Immunity · doi:10.1128/iai.62.8.3416-3423.1994 · Cited by 51
Koo, S.-P., Yeaman, M. R., & Bayer, A. S. (1996) · Infection and Immunity · doi:10.1128/iai.64.9.3758-3764.1996 · Cited by 42
Koo, S.-P., Yeaman, M. R., Nast, C. C., & Bayer, A. S. (1997) · Infection and Immunity · doi:10.1128/iai.65.11.4795-4800.1997 · Cited by 37
Nutrient transport in Candida albicans, a pathogenic yeast
Prasad, R. (1987) · Yeast · doi:10.1002/yea.320030402 · Cited by 31
Koo, S.-P., Bayer, A. S., Kagan, B. L., & Yeaman, M. R. (1999) · Infection and Immunity · doi:10.1128/iai.67.5.2475-2481.1999 · Cited by 31
Transport of basic amino acids in Candida albicans
Rao, L. R. S., Prasad, D. S., & Prasad, R. (1986) · Biochimica et Biophysica Acta (BBA) - Biomembranes · doi:10.1016/0005-2736(86)90033-7 · Cited by 18
Mishra, P., & Prasad, R. (1987) · PubMed · Cited by 16
Mishra, P., & Prasad, R. (1991) · Springer eBooks · doi:10.1007/978-3-642-75253-7_9 · Cited by 11
Homolog of a‐factor receptor gene in Saccharomyces exiguus
Kusumoto, K.-I., Hino, A., Yonekura, Y., Nogata, Y., Ohta, H., & Takano, H. (1998) · Yeast · doi:10.1002/(sici)1097-0061(19980430)14:6 · Cited by 1
Research works printed but not linked: 2
- 10.1001/archinte.1995.00430110089009 · DOI · Paper page
- Tang Y. Q. Yeaman M. R. Selsted M. E. Purification, characterization, and antimicrobial properties of peptides released from thrombin-induced human platelets.Blood861995556a (Abstract 3626.)
Cited by
Sorted by citations
Mechanisms of Antimicrobial Peptide Action and Resistance
Yeaman, M. R., & Yount, N. Y. (2003) · Pharmacological Reviews · doi:10.1124/pr.55.1.2 · Cited by 2,998
“Bayer et al. (2000) then compared parameters of membrane structure and function in genetically related S. aureus strains differing in tPMP-1 susceptibility or resistance.”
BackgroundResultEffect of essential oils on pathogenic bacteria
Nazzaro, F., Fratianni, F., De Martino, L., Coppola, R., & De Feo, V. (2013) · Pharmaceuticals · doi:10.3390/ph6121451 · Cited by 2,186
“An increase in the amount of SFAs in the membrane lipid bilayer results in a loss of membrane fluidity and a consequent increase in membrane rigidity [74].”
BackgroundImmune evasion by staphylococci
Foster, T. J. (2005) · Nature Reviews Microbiology · doi:10.1038/nrmicro1289 · Cited by 1,214
Mechanisms and consequences of bacterial resistance to antimicrobial peptides
Andersson, D. I., Hughes, D., & Kubicek-Sutherland, J. Z. (2016) · Drug Resistance Updates · doi:10.1016/j.drup.2016.04.002 · Cited by 718
“…membrane fluidity via expression of unsaturated lipids 5 sistan ( 1 7 a l f t m i l t r d T l f b t i s o o a f n d s i 2 r H i g c A a p c b i ( g r 2 s t L m i c i a a e a i i A t i t l 0 D.I. Andersson et al. / Drug Re Bayer et al., 2000) or reducing membrane potential (Yeaman et al., 998).”
BackgroundMembrane toxicity of antimicrobial compounds from essential oils
Di Pasqua, R., Betts, G., Hoskins, N., Edwards, M., Ercolini, D., & Mauriello, G. (2007) · Journal of Agricultural and Food Chemistry · doi:10.1021/jf0636465 · Cited by 648
Metadata
- Authors
- Bayer, A. S., Prasad, R., Chandra, J., Koul, A., Smriti, M., Varma, A., Skurray, R. A., Firth, N., Brown, M. H., Koo, S.-P., & Yeaman, M. R.
- Affiliations
- University of California, Los Angeles (US)
- Jawaharlal Nehru University (IN)
- The University of Sydney (AU)
- ORCID
- Venue
- Infection and Immunity
- Publisher
- American Society for Microbiology
- Published
- 2000-06
- Volume
- 68
- Issue
- 6
- Pages
- 3548-3553
- ISSN
- 0019-9567 (ISSN-L)
- 1098-5522
- PMC
- PMC97641
- OpenAlex
- W2156172406
- Cited by
- 150
- References
- 52
- Citations by year
- 2026: 1 · 2025: 4 · 2024: 4 · 2023: 4 · 2022: 2 · 2021: 8 · 2020: 5 · 2019: 5 · 2018: 7 · 2017: 7 · 2016: 11 · 2015: 7 · 2014: 6 · 2013: 9 · 2012: 14
- FWCI
- 3.64
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Infection and Immunity (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health
- National Institute of Allergy and Infectious Diseases (AI 39108, AI-39001, R01AI039108, R29AI039001, R01AI039001)
- Topics
- Health Sciences › Medicine › Infectious Diseases › Antimicrobial Resistance in Staphylococcus
- Life Sciences › Immunology and Microbiology › Microbiology › Antimicrobial Peptides and Activities
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Keywords
- Staphylococcus aureus, antimicrobial resistance, membrane lipid composition, endocarditis, cationic antimicrobial peptides, multiresistance plasmid
- MeSH terms
- Amino Acids, Anti-Bacterial Agents, beta-Thromboglobulin, Biological Transport, Blood Proteins, Cell Membrane, DNA Transposable Elements, Drug Resistance, Microbial, Fatty Acids, Membrane Fluidity, Phenotype, Phospholipids, Species Specificity, Staphylococcus aureus, Mutagenesis, Insertional, Chemokines
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
