The DOI matches its Crossref record; 45 of the 45 references it cites were found in a registry or an index.
Identification and Characterization of the Hemophore-Dependent Heme Acquisition System of Yersinia pestis
Abstract
Yersinia pestis possesses a heme-protein acquisition system (Hmu) that allows it to utilize heme and heme-protein complexes as the sole sources of iron. Analysis of the Y. pestis CO92 genomic sequence revealed a second heme-protein acquisition gene cluster that shares homology with the hemophore-dependent heme acquisition system (Has system) of Serratia marcescens . This locus consisted of the hasR yp receptor gene, the hasA yp hemophore gene, and genes encoding components of the HasA yp dedicated ABC transporter factor ( hasDE yp ), as well as a tonB homologue ( hasB yp ). By using a reconstituted secretion system in Escherichia coli , we showed that HasA yp is a secreted heme-binding protein and that expression of HasA yp is iron regulated in E. coli . The use of a transcriptional reporter fusion showed that the hasRADEB promoter is Fur regulated and has increased activity at 37°C. Hemoglobin utilization via the Has yp system was studied with both E. coli and Y. pestis , for which has and has hmu mutant strains were used. No contribution of the Has system to heme utilization was observed in either E. coli or Y. pestis under the conditions we tested. Previously it was shown that a deletion of the Hmu system had no effect on the virulence of Y. pestis in a mouse model of bubonic plague. An Hmu − Has − double mutant also retained full virulence in this model of infection. This report constitutes the first attempt to investigate the contribution of the hemophore-dependent heme acquisition system in bacterial pathogenicity.
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“The HasA hemophore-mediated heme uptake system has been identified in Serratia marcescens , P. aeruginosa , Pseudomonas fluorescens , and Y. pestis (4, 45, 56, 58, 96).”
From Overcoming the Heme Paradox: Heme Toxicity and Tolerance in Bacterial Pathogens (2010) · cites this paper as [96]
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Molecular Cloning: A Laboratory Manual
Sambrook, J., Fritsch, E., & Maniatis, T. (1989) · UCLA - Biblioteca de Ciencias de la Salud · Cited by 133,589
DNA sequencing with chain-terminating inhibitors
Sanger, F., Nicklen, S., & Coulson, A. R. (1977) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.74.12.5463 · Cited by 68,572
A SIMPLE METHOD OF ESTIMATING FIFTY PER CENT ENDPOINTS12
REED, L. J., & MUENCH, H. (1938) · American Journal of Epidemiology · doi:10.1093/oxfordjournals.aje.a118408 · Cited by 19,926
A simple method of estimating 50 per cent end points
Muench, H. (1938) · Medical Entomology and Zoology · Cited by 8,376
Chang, A. C. Y., & Cohen, S. N. (1978) · Journal of Bacteriology · doi:10.1128/jb.134.3.1141-1156.1978 · Cited by 4,181
Miller, J. H. (1992) · Medical Entomology and Zoology · Cited by 2,488
[22] Using CLUSTAL for multiple sequence alignments
Higgins, D. G., Thompson, J., & Gibson, T. J. (1996) · Methods in enzymology on CD-ROM/Methods in enzymology · doi:10.1016/s0076-6879(96)66024-8 · Cited by 1,577
Marck, C. (1988) · Nucleic Acids Research · doi:10.1093/nar/16.5.1829 · Cited by 986
Kaniga, K., Delor, I., & Cornelis, G. R. (1991) · Gene · doi:10.1016/0378-1119(91)90599-7 · Cited by 698
Kanamycin-resistant vectors that are analogues of plasmids pUC8, pUC9, pEMBL8 and pEMBL9
Spratt, B. G., Hedge, P., Heesen, S. T., Edelman, A., & Broome‐Smith, J. K. (1986) · Gene · doi:10.1016/0378-1119(86)90117-4 · Cited by 425
Research works printed but not linked: 3
- Paquelin A. J. M. Ghigo S. Bertin and C. Wandersma. Characterisation of HasB a Serratia marcescens TonB-like protein specifically involved in the hemophore-dependent heme acquisition system. Mol. Microbiol. in press.
- Perry R. D. Bearden S. W. Fetherston J. D. Iron and heme acquisition storage systems in Yersinia pestis.Recent Res. Dev. Microbiol.520011327
- Reed L. J. Muench H. A simple method of estimating fifty percent endpoints.Am. J. Hyg.271938493497
Cited by
Shared and distinct mechanisms of iron acquisition by bacterial and fungal pathogens of humans
Caza, M., & Kronstad, J. W. (2013) · Frontiers in Cellular and Infection Microbiology · doi:10.3389/fcimb.2013.00080 · Cited by 306
Overcoming the Heme Paradox: Heme Toxicity and Tolerance in Bacterial Pathogens
Anzaldi, L. L., & Skaar, E. P. (2010) · Infection and Immunity · doi:10.1128/iai.00613-10 · Cited by 295
Iron acquisition strategies of bacterial pathogens
Sheldon, J. R., Laakso, H. A., & Heinrichs, D. E. (2016) · Microbiology Spectrum · doi:10.1128/microbiolspec.vmbf-0010-2015 · Cited by 219
Discovery and characterization of a unique mycobacterial heme acquisition system
Tullius, M. V., Harmston, C. A., Owens, C. P., Chim, N., Morse, R. P., McMath, L. M., Iniguez, A., Kimmey, J. M., Sawaya, M. R., Whitelegge, J. P., et al. (2011) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1009516108 · Cited by 199
Bacterial heme-transport proteins and their heme-coordination modes
Tong, Y., & Guo, M. (2009) · Archives of Biochemistry and Biophysics · doi:10.1016/j.abb.2008.10.013 · Cited by 162
Metadata
- Authors
- Rossi, M.-S., Fetherston, J. D., Létoffé, S., Carniel, E., Perry, R. D., & Ghigo, J.-M.
- Affiliations
- <!--label omitted: 1-->Unité des Membranes Bactériennes Institut Pasteur (CNRS URA 2172)1 and
- <!--label omitted: 2-->Department of Microbiology and Immunology, University of Kentucky, Lexington, Kentucky 40536-02982
- <!--label omitted: 3-->Unité de Bactériologie Moléculaire et Médicale, Laboratoire des Yersinia,3 75724 Paris Cedex 15, France, and
- Venue
- Infection and Immunity
- Publisher
- American Society for Microbiology
- Published
- 2001-11
- Volume
- 69
- Issue
- 11
- Pages
- 6707-6717
- ISSN
- 0019-9567
- 1098-5522
- Cited by
- 91
- References
- 45
- Language
- en
- Licence
- journals.asm.org/non-commercial-tdm-license (tdm)
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- Rossi, M.-S., Fetherston, J. D., Létoffé, S., Carniel, E., Perry, R. D., & Ghigo, J.-M. (2001). Identification and Characterization of the Hemophore-Dependent Heme Acquisition System of Yersinia pestis. Infection and Immunity. https://doi.org/10.1128/iai.69.11.6707-6717.2001
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