Cited by 246Yersinia bacterium, plague, ectoparasites researchImmune Response and InflammationBacterial Infections and Vaccines

Modification of the Structure and Activity of Lipid A in Yersinia pestis Lipopolysaccharide by Growth Temperature

Open access
Kawahara, Kazuyoshi, Tsukano, Hiroko, Watanabe, Haruo, Lindner, Buko, Matsuura, Motohiro·2002·Infection and Immunity

Abstract

Yersinia pestis strain Yreka was grown at 27 or 37°C, and the lipid A structures (lipid A-27°C and lipid A-37°C) of the respective lipopolysaccharides (LPS) were investigated by matrix-assisted laser desorption ionization-time-of-flight (MALDI-TOF) mass spectrometry. Lipid A-27°C consisted of a mixture of tri-acyl, tetra-acyl, penta-acyl, and hexa-acyl lipid A's, of which tetra-acyl lipid A was most abundant. Lipid A-37°C consisted predominantly of tri- and tetra-acylated molecules, with only small amounts of penta-acyl lipid A; no hexa-acyl lipid A was detected. Furthermore, the amount of 4-amino-arabinose was substantially higher in lipid A-27°C than in lipid A-37°C. By use of mouse and human macrophage cell lines, the biological activities of the LPS and lipid A preparations were measured via their abilities to induce production of tumor necrosis factor alpha (TNF-α). In both cell lines the LPS and the lipid A from bacteria grown at 27°C were stronger inducers of TNF-α than those from bacteria grown at 37°C. However, the difference in activity was more prominent in human macrophage cells. These results suggest that in order to reduce the activation of human macrophages, it may be more advantageous for Y. pestis to produce less-acylated lipid A at 37°C.

Integrity signals

Registry

Crossref · 10.1128/iai.70.8.4092-4098.2002

Cites

46 of 46 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

We couldn’t retrieve this paper’s full text, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

  • In humans, hexa-acylated LPS potently activates TLR4 and host immunity, whereas hypo-acylated penta- and tetra-acylated LPS poorly activate TLR4 and can antagonize immune activation by hexa-acylated LPS.

    “In humans, hexa-acylated LPS potently activates TLR4 and host immunity, whereas hypo-acylated penta- and tetra-acylated LPS poorly activate TLR4 and can antagonize immune activation by hexa-acylated LPS6–9.”

Cites

Flagged first, then by citations

Cited by

Sorted by citations

Authors
Kawahara, K., Tsukano, H., Watanabe, H., Lindner, B., & Matsuura, M.
Affiliations
  • Department of Bacteriology, The Kitasato Institute, Tokyo 108-8642
  • National Institute of Infectious Diseases (JP)
  • Research Center Borstel - Leibniz Lung Center (DE)
  • Jichi Medical University (JP)
Venue
Infection and Immunity
Published
2002-08
Volume
70
Issue
8
Pages
4092-4098
ISSN
  • 0019-9567 (ISSN-L)
  • 1098-5522
OpenAlex
W2132032241
Cited by
246
References
46
Citations by year
2026: 5 · 2025: 2 · 2024: 5 · 2023: 8 · 2022: 7 · 2021: 11 · 2020: 4 · 2019: 8 · 2018: 7 · 2017: 9 · 2016: 14 · 2015: 8 · 2014: 14 · 2013: 17 · 2012: 19
FWCI
3.73
Type
journal article
Language
en
Open access
Locations
  • Infection and Immunity (publishedVersion)
  • Europe PMC (PubMed Central) (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Yersinia bacterium, plague, ectoparasites research
  • Life Sciences › Immunology and Microbiology › Immunology › Immune Response and Inflammation
  • Life Sciences › Immunology and Microbiology › Microbiology › Bacterial Infections and Vaccines
Keywords
Yersinia pestis, lipid A, lipopolysaccharide, growth temperature, acylation, TNF-α, macrophage activation, bacterial virulence
MeSH terms
Animals, Carbohydrate Conformation, Cell Line, Humans, Lipid A, Lipopolysaccharides, Macrophages, Structure-Activity Relationship, Temperature, Tumor Necrosis Factor-alpha, Yersinia pestis, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, U937 Cells, Mice

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review