The DOI matches its Crossref record; 27 of the 27 references it cites were found in a registry or an index.
Characterization of the Ends and Target Site of a Novel Tetracycline Resistance-Encoding Conjugative Transposon from Enterococcus faecium 664.1H1
Open accessAbstract
Enterococcus faecium 664.1H1 is multiply antibiotic resistant and mercury resistant. In this study, the genetic support for the tetracycline resistance of E. faecium 664.1H1 was characterized. The tet (S) gene is responsible for tetracycline resistance, and this gene is located on the chromosome of E. faecium 664.1H1, on a novel conjugative transposon. The element is transferable to Enterococcus faecalis , where it integrates into a specific site. The element was designated EfcTn 1 . The integrase of EfcTn 1 is related to the integrase proteins found on staphylococcal pathogenicity islands. We show that the transposon is flanked by an 18-bp direct repeat, a copy of which is also present at the target site and at the joint of a circular form, and we propose a mechanism of insertion and excision.
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In some eubacteria and archaea, transposon-mediated LGT usually generates a pair of DRs varying from several to a dozen base pairs flanking the foreign gene after its integration into the host genome.
“In some eubacteria and archaea, such as Acinetobacter baumannii, Escherichia coli, Enterococcus faecium, Lactococcus lactis, and Mycobacterium smegmatis, transposon-mediated LGT usually generates a pair of DRs varying from several to a dozen base pairs flanking the foreign gene after its integration into the host genome.33–36”
From Whole genomic DNA sequencing and comparative genomic analysis of Arthrospira platensis: high genome plasticity and genetic diversity (2016) · cites this paper as [36]
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Cites
Chung, C. T., Niemelä, S., & Miller, R. H. (1989) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.86.7.2172 · Cited by 1,602
Multiplex PCR for the detection of tetracycline resistant genes
Ng, L. ‐., Martín, I., Alfa, M. J., & Mulvey, M. R. (2001) · Molecular and Cellular Probes · doi:10.1006/mcpr.2001.0363 · Cited by 872
Multiple-Drug Resistant Enterococci: The Nature of the Problem and an Agenda for the Future
Huycke, M. M., Sahm, D. F., & Gilmore, M. S. (1998) · Emerging infectious diseases · doi:10.3201/eid0402.980211 · Cited by 717
Jacob, A. E., & Hobbs, S. J. (1974) · Journal of Bacteriology · doi:10.1128/jb.117.2.360-372.1974 · Cited by 682
Giraffa, G. (2002) · FEMS Microbiology Reviews · doi:10.1111/j.1574-6976.2002.tb00608.x · Cited by 620
Structure of Cre recombinase complexed with DNA in a site-specific recombination synapse
Guo, F., Gopaul, D. N., & Duyne, G. D. V. (1997) · Nature · doi:10.1038/37925 · Cited by 573
Aarestrup, F. M., Agersø, Y., Gerner‐Smidt, P., Madsen, M., & Jensen, L. (2000) · Diagnostic Microbiology and Infectious Disease · doi:10.1016/s0732-8893(00)00130-9 · Cited by 480
Similarities and differences among 105 members of the Int family of site-specific recombinases
Nunes-Düby, S. E., Kwon, H. J., Tirumalai, R. S., Ellenberger, T., & Landy, A. (1998) · Nucleic Acids Research · doi:10.1093/nar/26.2.391 · Cited by 448
Relationships between Enterococcal Virulence and Antimicrobial Resistance
Mundy, L. M., Sahm, D. F., & Gilmore, M. S. (2000) · Clinical Microbiology Reviews · doi:10.1128/cmr.13.4.513 · Cited by 440
Fitzgerald, J. R., Monday, S. R., Foster, T. J., Bohach, G. A., Hartigan, P. J., Meaney, W. J., & Smyth, C. J. (2001) · Journal of Bacteriology · doi:10.1128/jb.183.1.63-70.2001 · Cited by 280
Antibiotic resistance spread in food
Perreten, V., Schwarz, F., Cresta, L., Boeglin, M., Dasen, G., & Teuber, M. (1997) · Nature · doi:10.1038/39767 · Cited by 267
Tn 916 Family Conjugative Transposons and Dissemination of Antimicrobial Resistance Determinants
Rice, L. B. (1998) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.42.8.1871 · Cited by 259
Summers, A. O., Wireman, J., Vimy, M. J., Lorscheider, F. L., Marshall, B. M., Levy, S. B., Bennett, S., & Billard, L. (1993) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.37.4.825 · Cited by 249
Identification and antimicrobial susceptibility of enterococci isolated from the root canal
Dahlén, G., Samuelsson, W., Molander, A., & Reit, C. (2000) · Oral Microbiology and Immunology · doi:10.1034/j.1399-302x.2000.150507.x · Cited by 215
Antibiotic-resistant enterococci: the mechanisms and dynamics of drug introduction and resistance
Shepard, B. D., & Gilmore, M. S. (2002) · Microbes and Infection · doi:10.1016/s1286-4579(01)01530-1 · Cited by 202
Nucleotide Sequence of the 18-kb Conjugative Transposon Tn916 from Enterococcus faecalis
Flannagan, S. E., Zitzow, L. A., Su, Y. A., & Clewell, D. B. (1994) · Plasmid · doi:10.1006/plas.1994.1077 · Cited by 196
Butaye, P., Devriese, L. A., & Haesebrouck, F. (2001) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.45.5.1374-1378.2001 · Cited by 157
Úbeda, C., Tormo‐Más, M. Á., Cucarella, C., Trotonda, M. P., Foster, T. J., Lasa, Í., & Penadés, J. R. (2003) · Molecular Microbiology · doi:10.1046/j.1365-2958.2003.03577.x · Cited by 126
Charpentier, E., Gerbaud, G., & Courvalin, P. M. (1993) · Gene · doi:10.1016/0378-1119(93)90665-p · Cited by 93
Structure and mechanism in site-specific recombination
Gopaul, D. N., & Duyne, G. D. V. (1999) · Current Opinion in Structural Biology · doi:10.1016/s0959-440x(99)80003-7 · Cited by 92
Breedlove, S. M. (1997) · Nature · doi:10.1038/39764 · Cited by 89
Presence of the Listeria tetracycline resistance gene tet(S) in Enterococcus faecalis
Charpentier, E., Gerbaud, G., & Courvalin, P. M. (1994) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.38.10.2330 · Cited by 71
Lancaster, H., Roberts, A. P., Bedi, R., Wilson, M., & Mullany, P. P. (2004) · Journal of Bacteriology · doi:10.1128/jb.186.13.4395-4398.2004 · Cited by 54
Self-citationExcision of a conjugative transposon in vitro by the Int and Xis proteins of Tn916
Rudy, C. K. (1997) · Nucleic Acids Research · doi:10.1093/nar/25.20.4061 · Cited by 49
[6] Gene transfer in bacterial biofilms
Roberts, A. P., Mullany, P. P., & Wilson, M. A. (2001) · Methods in enzymology on CD-ROM/Methods in enzymology · doi:10.1016/s0076-6879(01)36578-3 · Cited by 45
Self-citationWu, Z., Gumport, R. I., & Gardner, J. F. (1998) · Journal of Molecular Biology · doi:10.1006/jmbi.1998.1963 · Cited by 30
Xis protein of the conjugative transposon Tn916 plays dual opposing roles in transposon excision
Hinerfeld, D. A., & Churchward, G. G. (2001) · Molecular Microbiology · doi:10.1046/j.1365-2958.2001.02626.x · Cited by 22
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Horizontal Gene Transfer of Antibiotic Resistance Genes in Biofilms
Michaelis, C., & Grohmann, E. (2023) · Antibiotics · doi:10.3390/antibiotics12020328 · Cited by 700
Hegstad, K., Mikalsen, T., Coque, T. M., Werner, G., & Sundsfjord, A. (2010) · Clinical Microbiology and Infection · doi:10.1111/j.1469-0691.2010.03226.x · Cited by 377
Roberts, A. P., & Mullany, P. P. (2011) · FEMS Microbiology Reviews · doi:10.1111/j.1574-6976.2011.00283.x · Cited by 238
Self-citationA modular master on the move: the Tn916 family of mobile genetic elements
Roberts, A. P., & Mullany, P. P. (2009) · Trends in Microbiology · doi:10.1016/j.tim.2009.03.002 · Cited by 242
Self-citationAntibiotic resistant enterococci—Tales of a drug resistance gene trafficker
Werner, G., Coque, T. M., Franz, C. M. A. P., Grohmann, E., Hegstad, K., Jensen, L., van Schaik, W., & Weaver, K. E. (2013) · International Journal of Medical Microbiology · doi:10.1016/j.ijmm.2013.03.001 · Cited by 194
Seville, L., Patterson, A. J., Scott, K. P., Mullany, P. P., Quail, M. A., Parkhill, J., Ready, D. R., Wilson, M., Spratt, D. A., & Roberts, A. P. (2009) · Microbial Drug Resistance · doi:10.1089/mdr.2009.0916 · Cited by 125
Self-citationRieke, E. L., Ibekwe, A. M., Zilles, J. L., & Pruden, A. J. (2016) · Journal of Environmental Quality · doi:10.2134/jeq2015.07.0367 · Cited by 109
“Multiple studies have confirmed the presence of tetracycline and macrolide ARGs in transposable elements using PCR and sequencing techniques (Roberts et al., 2006; Cochetti et al., 2008; Del Grosso et al., 2009).”
MethodExtrachromosomal and mobile elements in enterococci: transmission, maintenance, and epidemiology
Clewell, D. B., Weaver, K. E., Dunny, G. M., Coque, T. M., Francia, M. V., & Hayes, F. (2014) · Research Explorer (The University of Manchester) · Cited by 106
Fitness costs of various mobile genetic elements in Enterococcus faecium and Enterococcus faecalis
Starikova, I., Al‐Haroni, M., Werner, G., Roberts, A. P., Sørum, V., Nielsen, K. M., & Johnsen, P. J. (2013) · Journal of Antimicrobial Chemotherapy · doi:10.1093/jac/dkt270 · Cited by 78
Self-citationDemonstration of in vivo transfer of doxycycline resistance mediated by a novel transposon
Warburton, P., Palmer, R. M. J., Munson, M. A., & Wade, W. G. (2007) · Journal of Antimicrobial Chemotherapy · doi:10.1093/jac/dkm331 · Cited by 65
“Transposons have often been identified as belonging to the Tn916 family solely on the basis of the presence of tet(M) and/or Int-Tn genes.31,32 Although these two genes are present on the majority of the transposons belonging to the Tn916 family, the group also contain transposons that do not carry the tet(M) gene, su…”
BackgroundSantoro, F., Vianna, M. E., & Roberts, A. P. (2014) · Frontiers in Microbiology · doi:10.3389/fmicb.2014.00535 · Cited by 64
“…resistance gene composed of tet(S) (encoding 599 amino acids of the gene) from a related element from Enterococcus casseliflavus designated Tn6000 (Roberts et al., 2006; Brouwer et al., 2010) and the Tn916 wildtype tet(M) gene (encoding 61 amino acids of the hybrid gene) and designated tet(S/M)…”
BackgroundSelf-citationXu, T., Qin, S., Hu, Y., Song, Z., Ying, J., Li, P., Dong, W., Zhao, F., Yang, H., & Bao, Q. (2016) · DNA Research · doi:10.1093/dnares/dsw023 · Cited by 37
Ciric, L., Brouwer, M. S. M., Mullany, P. P., & Roberts, A. P. (2014) · FEMS Microbiology Letters · doi:10.1111/1574-6968.12410 · Cited by 37
“The resulting amplicon was sequenced and shown to be identical to tet(S) from the conjugative transposon Tn6000 (FN555436; Roberts et al., 2006; Brouwer et al., 2010).”
BackgroundMethodInfluentialSelf-citationLi, X., Alvárez, V. B., Harper, W. J., & Wang, H. H. (2011) · Applied and Environmental Microbiology · doi:10.1128/aem.05168-11 · Cited by 34
Maki, T., Santos, M. D., Kondo, H., Hırono, I., & Aoki, T. (2009) · Applied and Environmental Microbiology · doi:10.1128/aem.00039-09 · Cited by 23
“ermB1 is located next to an MLS leader peptide, which is known to regulate erm gene expression in the absence of EM (8), in the first 20-kb region, while the ermB2 gene is located in the plasmid’s second 12-kb region downstream of the tetracycline resistance gene tetS.”
BackgroundDifferent Genetic Supports for the tet (S) Gene in Enterococci
Novais, C., Freitas, A. R., Silveira, E., Baquero, F., Peixe, L. M. S. V., Roberts, A. P., & Coque, T. M. (2012) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.00758-12 · Cited by 17
“performed as described (15) and revealed that types “B” and “C” use the gene coding for 94 the L31 ribosomal protein as the hot spot for integration, as reported for the original 95 CTn6000 “type A” (15).”
BackgroundMethodSelf-citationPersistence of endodontic infection and Enterococcus faecalis: Role of horizontal gene transfer
Jain, H., Mulay, S., & Mullany, P. P. (2016) · Gene Reports · doi:10.1016/j.genrep.2016.09.010 · Cited by 16
Self-citationPrevalence, diversity and transferability of the Tn 916 -Tn 1545 family ICE in oral streptococci
Lunde, T. M., Hjerde, E., & Al‐Haroni, M. (2021) · Journal of Oral Microbiology · doi:10.1080/20002297.2021.1896874 · Cited by 16
Brouwer, M. S. M., Mullany, P. P., & Roberts, A. P. (2010) · FEMS Microbiology Letters · doi:10.1111/j.1574-6968.2010.02018.x · Cited by 16
“The DNA sequence of the remainder of the element has been reported previously (Roberts et al., 2006) and includes tet(S) and the Tn6000 integrase.”
BackgroundMethodInfluentialSelf-citationEvolutionary Biology of Pathogenic Enterococci
Coque, T. M. (2014) · ASM Press eBooks · doi:10.1128/9781555815639.ch42 · Cited by 12
Probiotic potential of African fermented millet
Lei, V. (2015) · Cited by 9
Antimicrobial Resistance in the Enterococcus
Eliopoulos, G. M. (2007) · CRC Press eBooks · doi:10.1201/9781420008753.ch11 · Cited by 4
Survey of Enterococcus faecalis in Diseased Swine in Hunan Province, China
Jiang, W., Tian, S.-C., Li, R., Yun-qiu, Y., Wang, Y., & Li, M. (2011) · The Thai Journal of Veterinary Medicine · doi:10.56808/2985-1130.2324 · Cited by 1
“Additionally, E. faecalis can harbor antibiotics resistance genes, virulence factors and other mobile elements and transfer to other bacteria as a gene reservoir, through the food chain (Roberts et al., 2006; Newell et al., 2010).”
Background
Metadata
- Authors
- Roberts, A. P., Davis, I. J., Seville, L., Villedieu, A., & Mullany, P.
- Affiliations
- University College London (GB)
- Corresponding author
- Roberts, Adam P.
- Venue
- Journal of Bacteriology
- Publisher
- American Society for Microbiology
- Published
- 2006-06-15
- Volume
- 188
- Issue
- 12
- Pages
- 4356-4361
- ISSN
- 0021-9193 (ISSN-L)
- 1098-5530
- OpenAlex
- W2089522365
- Cited by
- 24
- References
- 27
- Citations by year
- 2023: 1 · 2021: 1 · 2016: 3 · 2015: 1 · 2014: 4 · 2013: 2 · 2012: 1
- FWCI
- 0.85
- Type
- journal article
- Language
- en
- Open access
- green
- europepmc.org
- Locations
- Journal of Bacteriology (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed (publishedVersion)
- UCL Discovery (University College London) (submittedVersion)
- Topics
- Health Sciences › Medicine › Microbiology (medical) › Antibiotic Resistance in Bacteria
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Health Sciences › Medicine › Infectious Diseases › Antimicrobial Resistance in Staphylococcus
- Keywords
- Enterococcus faecium, tetracycline resistance, conjugative transposons, transposon ends, integrase, direct repeats, Enterococcus faecalis, mercury resistance, antibiotic resistance
- MeSH terms
- Amino Acid Sequence, Chromosomes, Bacterial, Conjugation, Genetic, DNA Transposable Elements, Genes, Bacterial, Molecular Sequence Data, Tetracycline, Tetracycline Resistance, Sequence Alignment, Enterococcus faecium, Integrases
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