Preferential Reduction of the Thermodynamically Less Favorable Electron Acceptor, Sulfate, by a Nitrate-Reducing Strain of the Sulfate-Reducing Bacterium Desulfovibrio desulfuricans 27774
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Desulfovibrio desulfuricans strain 27774 is one of a relative small group of sulfate-reducing bacteria that can also grow with nitrate as an alternative electron acceptor, but how nitrate reduction is regulated in any sulfate-reducing bacterium is controversial. Strain 27774 grew more rapidly and to higher yields of biomass with nitrate than with sulfate or nitrite as the only electron acceptor. In the presence of both sulfate and nitrate, sulfate was used preferentially, even when cultures were continuously gassed with nitrogen and carbon dioxide to prevent sulfide inhibition of nitrate reduction. The napC transcription start site was identified 112 bases upstream of the first base of the translation start codon. Transcripts initiated at the napC promoter that were extended across the napM-napA boundary were detected by reverse transcription-PCR, confirming that the six nap genes can be cotranscribed as a single operon. Real-time PCR experiments confirmed that nap operon expression is regulated at the level of mRNA transcription by at least two mechanisms: nitrate induction and sulfate repression. We speculate that three almost perfect inverted-repeat sequences located upstream of the transcription start site might be binding sites for one or more proteins of the CRP/FNR family of transcription factors that mediate nitrate induction and sulfate repression of nitrate reduction by D. desulfuricans .
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Organisms lacking the napB gene, such as Desulfovibrio desulfuricans, encode alternative iron cofactor redox partners such as NapM or NapC.
“Organisms lacking the napB gene, such as Desulfovibrio desulfuricans, encode alternative iron cofactor redox partners, such as NapM or NapC.249,250”
From Nitrate and periplasmic nitrate reductases (2014) · cites this paper as [250]
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Nitrate and periplasmic nitrate reductases
Sparacino-Watkins, C., Stolz, J. F., & Basu, P. (2014) · Chem. Soc. Rev. · doi:10.1039/c3cs60249d · Cited by 426 · see citing works
- Authors
- Marietou, A., Griffiths, L., & Cole, J.
- Affiliations
- University of Birmingham (GB)
- ORCID
- Marietou, Angeliki
- Venue
- Journal of Bacteriology
- Publisher
- American Society for Microbiology
- Published
- 2009-02
- Volume
- 191
- Issue
- 3
- Pages
- 882-889
- ISSN
- 0021-9193 (ISSN-L)
- 1098-5530
- PMC
- PMC2632061
- OpenAlex
- W2141724754
- Cited by
- 46
- References
- 41
- Citations by year
- 2026: 1 · 2025: 2 · 2024: 1 · 2023: 2 · 2022: 4 · 2021: 4 · 2020: 3 · 2019: 1 · 2018: 1 · 2017: 3 · 2016: 4 · 2015: 2 · 2014: 6 · 2013: 2 · 2012: 4
- FWCI
- 1.14
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Journal of Bacteriology (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- University of Birmingham Research Portal (University of Birmingham) (submittedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Physical Sciences › Environmental Science › Environmental Engineering › Microbial Fuel Cells and Bioremediation
- Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Metalloenzymes and iron-sulfur proteins
- Keywords
- nitrate reduction, sulfate reduction, nitrate induction, inverted repeats, gene expression regulation, sulfate-reducing bacteria
- MeSH terms
- Bacterial Proteins, Binding Sites, Electrophoresis, Polyacrylamide Gel, Nitrates, Nitrites, Sulfates, Blotting, Western, Polymerase Chain Reaction, Reverse Transcriptase Polymerase Chain Reaction, Transcription Initiation Site, Desulfovibrio desulfuricans
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