CoChat
Sign Up
Cited by 147Bacterial Genetics and BiotechnologyOral microbiology and periodontitis researchMicrobial metabolism and enzyme function

CcpA Regulates Central Metabolism and Virulence Gene Expression in Streptococcus mutans

Open access
Abranches, Jacqueline, Nascimento, Marcelle M., Zeng, Lin, Browngardt, Christopher M., Wen, Zezhang T., Rivera, Mercedes F., Burne, Robert A.·2008·Journal of Bacteriology
DOI 10.1128/jb.01237-07PMC PMC2293215
Open this paper in CoChat — free account

Abstract

CcpA globally regulates transcription in response to carbohydrate availability in many gram-positive bacteria, but its role in Streptococcus mutans remains enigmatic. Using the fructan hydrolase ( fruA ) gene of S. mutans as a model, we demonstrated that CcpA plays a direct role in carbon catabolite repression (CCR). Subsequently, the expression of 170 genes was shown to be differently expressed (≥2-fold) in glucose-grown wild-type (UA159) and CcpA-deficient (TW1) strains ( P ≤ 0.001). However, there were differences in expression of only 96 genes between UA159 and TW1 when cells were cultivated with the poorly repressing substrate galactose. Interestingly, 90 genes were expressed differently in wild-type S. mutans when glucose- and galactose-grown cells were compared, but the expression of 515 genes was altered in the CcpA-deficient strain in a similar comparison. Overall, our results supported the hypothesis that CcpA has a major role in CCR and regulation of gene expression but revealed that in S. mutans there is a substantial CcpA-independent network that regulates gene expression in response to the carbohydrate source. Based on the genetic studies, biochemical and physiological experiments demonstrated that loss of CcpA impacts the ability of S. mutans to transport and grow on selected sugars. Also, the CcpA-deficient strain displayed an enhanced capacity to produce acid from intracellular stores of polysaccharides, could grow faster at pH 5.5, and could acidify the environment more rapidly and to a greater extent than the parental strain. Thus, CcpA directly modulates the pathogenic potential of S. mutans through global control of gene expression.

Integrity signals

Registry

Crossref · 10.1128/jb.01237-07

Cites

56 of 56 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

We couldn’t retrieve this paper’s full text, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

  • The carbohydrate catabolite repression gene ccpA is located upstream of the S mutans bacteriocin production regulon and regulates its expression by controlling expression of S mutans competence stimulating peptide.

    “The carbohydrate catabolite repression gene, catabolite control protein A (ccpA), is located upstream of the S mutans bacteriocin production regulon and has been shown to regulate the expression of this by controlling the expression of S mutans competence stimulating peptide.”

    From The oral microbiome: Role of key organisms and complex networks in oral health and disease (2021) · cites this paper as [292]

Cites

Flagged first, then by citations

Cited by

Sorted by citations

Related

Authors
Abranches, J., Nascimento, M. M., Zeng, L., Browngardt, C. M., Wen, Z. T., Rivera, M. F., & Burne, R. A.
Affiliations
  • University of Florida Health (US)
  • University of Florida (US)
Corresponding author
Burne, Robert A.
ORCID
Venue
Journal of Bacteriology
Publisher
American Society for Microbiology
Published
2008-04
Volume
190
Issue
7
Pages
2340-2349
ISSN
  • 0021-9193 (ISSN-L)
  • 1098-5530
DOI
10.1128/jb.01237-07
PMC
PMC2293215
OpenAlex
W2132219663
Cited by
147
References
56
Citations by year
2026: 5 · 2025: 3 · 2024: 6 · 2023: 6 · 2022: 6 · 2021: 8 · 2020: 4 · 2019: 11 · 2018: 11 · 2017: 3 · 2016: 9 · 2015: 7 · 2014: 13 · 2013: 10 · 2012: 8
FWCI
7.05
Type
journal article
Language
en
Open access
Licence
journals.asm.org/non-commercial-tdm-license (tdm)
Locations
  • Journal of Bacteriology (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Funders
National Institute of Dental and Craniofacial Research (DE12236, R01DE012236)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
  • Health Sciences › Dentistry › Periodontics › Oral microbiology and periodontitis research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial metabolism and enzyme function
Keywords
California Consumer Privacy Act, Streptococcus mutans, catabolite repression, transcriptional regulation, virulence gene expression, central carbon metabolism, sugar transport, acid production, polysaccharide degradation, low pH growth, dental caries
MeSH terms
Bacterial Proteins, Biological Transport, DNA-Binding Proteins, Galactose, Glucose, Glycoside Hydrolases, Hydrogen-Ion Concentration, Polysaccharides, Repressor Proteins, Streptococcus mutans, Transcription, Genetic, Virulence, Gene Expression Regulation, Bacterial, Reverse Transcriptase Polymerase Chain Reaction, Oligonucleotide Array Sequence Analysis, Electrophoretic Mobility Shift Assay

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review