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Endonuclease from Micrococcus luteus Which Has Activity Toward Ultraviolet-Irradiated Deoxyribonucleic Acid: Purification and Properties
Open accessAbstract
An endonuclease purified ∼3,200-fold from Micrococcus luteus is active on native ultraviolet-irradiated deoxyribonucleic acid (DNA), but is inactive on unirradiated native or denatured DNA and has no activity toward irradiated denatured DNA. The major type of lesion for the nucleolytic activity is the cyclobutane pyrimidine dimer. The enzyme makes a number of single-strand breaks approximately equal to the number of dimers, but dimers are not excised. This endonuclease—a small molecular weight protein—therefore has all the attributes hypothesized for the first enzyme in the sequential steps in repair of DNA in vivo. Another paper shows that the endonuclease is able to reactivate ultraviolet-irradiated transforming DNA.
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Cited by
Action spectrum for DMA damage in alfalfa lowers predicted impact of ozone depletion
Quaite, F. E., Sutherland, B. M., & Sutherland, J. C. (1992) · Nature · doi:10.1038/358576a0 · Cited by 314
Freeman, S. E., Hacham, H., Gange, R. W., Maytum, D. J., Sutherland, J. C., & Sutherland, B. M. (1989) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.86.14.5605 · Cited by 311
DNA sequence dependence of closely opposed cyclobutyl pyrimidine dimers induced by UV radiation
Lam, L. H., & Reynolds, R. J. (1987) · Mutation Research · doi:10.1016/0027-5107(87)90266-1 · Cited by 20
Freeman, S. E., & Ryan, S. L. (1990) · Mutation Research/DNA Repair · doi:10.1016/0921-8777(90)90072-d · Cited by 19
Metadata
- Authors
- Carrier, W. L., & Setlow, R. B.
- Venue
- Journal of Bacteriology
- Publisher
- American Society for Microbiology
- Published
- 1970-04
- Volume
- 102
- Issue
- 1
- Pages
- 178-186
- ISSN
- 0021-9193 (ISSN-L)
- 1098-5530
- OpenAlex
- W1952168550
- Cited by
- 267
- References
- 0
- FWCI
- 7.36
- Type
- journal article
- Language
- en
- Open access
- green
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- CoChat. (2026). Citation check: Endonuclease from Micrococcus luteus Which Has Activity Toward Ultraviolet-Irradiated Deoxyribonucleic Acid: Purification and Properties. https://app.cochat.ai/papers/10.1128/jb.102.1.178-186.1970
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- Carrier, W. L., & Setlow, R. B. (1970). Endonuclease from Micrococcus luteus Which Has Activity Toward Ultraviolet-Irradiated Deoxyribonucleic Acid: Purification and Properties. Journal of Bacteriology. https://doi.org/10.1128/jb.102.1.178-186.1970
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.