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Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid
Open accessAbstract
Construction and characterization of a class of multicopy plasmid cloning vehicles containing the replication system of miniplasmid P15A are described. The constructed plasmids have cleavage sites within antibiotic resistance genes for a variety of commonly employed site-specific endonucleases, permitting convenient use of the insertional inactivation procedure for the selection of clones that contain hybrid DNA molecules. Although the constructed plasmids showed DNA sequence homology with the ColE1 plasmid within the replication region, were amplifiable by chloramphenicol or spectinomycin, required DNA polymerase I for replication, and shared other replication properties with ColE1, they were nevertheless compatible with ColE1. P15A-derived plasmids were not self-transmissible and were mobilized poorly by Hfr strains; however, mobilization was complemented by the presence of a ColE1 plasmid within the same cell.
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Cited by
Guzman, L. M., Belin, D., Carson, M. J., & Beckwith, J. (1995) · Journal of Bacteriology · doi:10.1128/jb.177.14.4121-4130.1995 · Cited by 4,935
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Rosano, G. L., & Ceccarelli, E. A. (2014) · Frontiers in Microbiology · doi:10.3389/fmicb.2014.00172 · Cited by 2,556
Engineering BioBrick vectors from BioBrick parts
Shetty, R. P., Endy, D., & Knight, T. F. (2008) · Journal of Biological Engineering · doi:10.1186/1754-1611-2-5 · Cited by 760
The ClosTron: a universal gene knock-out system for the genus Clostridium
Heap, J. T., Pennington, O. J., Cartman, S. T., Carter, G. P., & Minton, N. P. (2007) · Journal of Microbiological Methods · doi:10.1016/j.mimet.2007.05.021 · Cited by 663
The ZnuABC high‐affinity zinc uptake system and its regulator Zur in Escherichia coli
Patzer, S. I., & Hantke, K. (1998) · Molecular Microbiology · doi:10.1046/j.1365-2958.1998.00883.x · Cited by 490
Metadata
- Authors
- Chang, A. C., & Cohen, S. N.
- Venue
- Journal of Bacteriology
- Publisher
- American Society for Microbiology
- Published
- 1978-06
- Volume
- 134
- Issue
- 3
- Pages
- 1141-1156
- ISSN
- 0021-9193 (ISSN-L)
- 1098-5530
- OpenAlex
- W2141653906
- Cited by
- 4,243
- References
- 0
- FWCI
- 43.17
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Chang, A. C., & Cohen, S. N. (1978). Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid. Journal of Bacteriology. https://doi.org/10.1128/jb.134.3.1141-1156.1978
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