The DOI matches its Crossref record; 14 of the 14 references it cites were found in a registry or an index.
Mutations that alter the signal sequence of alkaline phosphatase in Escherichia coli
Open accessAbstract
A phoA-lacZ gene fusion was used to isolate mutants altered in the alkaline phosphatase signal sequence. This was done by selecting Lac+ mutants from a phoA-lacZ fusion strain that produces a membrane-bound hybrid protein and is unable to grow on lactose. Two such mutant derivatives were characterized. The mutations lie within the phoA portion of the fused gene and cause internalization of the hybrid protein. When the mutations were genetically recombined into an otherwise wild-type phoA gene, they interfered with export of alkaline phosphatase to the periplasm. The mutant alkaline phosphatase protein was found instead in the cytoplasm in precursor form. DNA sequence analysis demonstrated that both mutations lead to amino acid alterations in the signal sequence of alkaline phosphatase.
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Cites
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Self-citationSuppressor mutations that restore export of a protein with a defective signal sequence
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Cited by
Determinants of membrane protein topology.
Boyd, D., Manoil, C., & Beckwith, J. (1987) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.84.23.8525 · Cited by 295
Self-citationA PhoP-repressed gene promotes Salmonella typhimurium invasion of epithelial cells
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Nitric Oxide Formation by Escherichia coli
Corker, H., & Poole, R. K. (2003) · Journal of Biological Chemistry · doi:10.1074/jbc.m303282200 · Cited by 178
The Thioredoxin Superfamily: Redundancy, Specificity, and Gray-Area Genomics
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Metadata
- Authors
- Michaelis, S., Inouye, H., Oliver, D., & Beckwith, J.
- Venue
- Journal of Bacteriology
- Publisher
- American Society for Microbiology
- Published
- 1983-04
- Volume
- 154
- Issue
- 1
- Pages
- 366-374
- ISSN
- 0021-9193 (ISSN-L)
- 1098-5530
- OpenAlex
- W1948880364
- Cited by
- 277
- References
- 14
- FWCI
- 8.08
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Michaelis, S., Inouye, H., Oliver, D., & Beckwith, J. (1983). Mutations that alter the signal sequence of alkaline phosphatase in Escherichia coli. Journal of Bacteriology. https://doi.org/10.1128/jb.154.1.366-374.1983
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
