The DOI matches its Crossref record; 28 of the 28 references it cites were found in a registry or an index.
Plasmid insertion mutagenesis and lac gene fusion with mini-mu bacteriophage transposons
Open accessAbstract
Small bacteriophage Mu transposable elements containing the lac operon structural genes were constructed to facilitate the isolation and use of Mu insertions and lac gene fusions. These mini-Mu elements have selectable genes for either ampicillin or kanamycin resistance and can be used to form both transcriptional and translational lac gene fusions. Some of the mini-Mu-lac elements constructed are deleted for the Mu A and B transposition genes and form stable insertions that cannot undergo transposition unless complemented for these functions. A procedure was developed for selecting mini-Mu insertions specifically into plasmids, including commonly used high-copy-number cloning vectors such as pBR322. Mu insertions in pBR322 were found to be distributed around the plasmid, but insertions in certain regions occurred more frequently than in others.
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Cites
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Self-citationComplete Nucleotide Sequence of the Escherichia coli Plasmid pBR322
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Cited by
TolC, an Escherichia coli outer membrane protein required for hemolysin secretion.
Wandersman, C., & Delepelaire, P. (1990) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.87.12.4776 · Cited by 444
An RNA Sensor for Intracellular Mg2+
Cromie, M. J., Shi, Y., Latifi, T., & Groisman, E. A. (2006) · Cell · doi:10.1016/j.cell.2006.01.043 · Cited by 323
Rabin, R. S., Collins, L. A., & Stewart, V (1992) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.89.18.8701 · Cited by 44
Metadata
- Authors
- Castilho, B. A., Olfson, P., & Casadaban, M. J.
- Venue
- Journal of Bacteriology
- Publisher
- American Society for Microbiology
- Published
- 1984-05
- Volume
- 158
- Issue
- 2
- Pages
- 488-495
- ISSN
- 0021-9193 (ISSN-L)
- 1098-5530
- OpenAlex
- W1558547246
- Cited by
- 543
- References
- 28
- FWCI
- 23.58
- Type
- journal article
- Language
- en
- Open access
- green
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- Castilho, B. A., Olfson, P., & Casadaban, M. J. (1984). Plasmid insertion mutagenesis and lac gene fusion with mini-mu bacteriophage transposons. Journal of Bacteriology. https://doi.org/10.1128/jb.158.2.488-495.1984
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
