The DOI matches its Crossref record; 22 of the 22 references it cites were found in a registry or an index.
Genes for alkaline protease and neutral protease from Bacillus amyloliquefaciens contain a large open reading frame between the regions coding for signal sequence and mature protein
Open accessAbstract
The genes for alkaline protease (apr[BamP]) and neutral protease (npr[BamP]) from Bacillus amyloliquefaciens have been isolated and expressed in Bacillus subtilis. The DNA sequences of apr[BamP] and npr[BamP] revealed, in each case, the presence of a large open reading frame. The inferred amino acid sequence of either gene contained a signal sequence and an additional polypeptide sequence ('pro' sequence) preceding the mature protein. Based on DNA sequence, the start point of translation has been identified as amino acid residue - 107 for apr[BamP] and -221 for npr[BamP]. To demonstrate that the start point of translation of apr[BamP] in vivo is probably at codon -107, codon -103 (AAA) was changed to an ochre (TAA) by site-directed mutagenesis. Alkaline protease was produced from this ochre mutant derivative of apr[BamP] only when the host strain was Su+. The presence of a pro sequence may be common to all of the secreted proteases from bacilli.
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Metadata
- Authors
- Vasantha, N., Thompson, L. D., Rhodes, C., Banner, C., Nagle, J., & Filpula, D.
- Venue
- Journal of Bacteriology
- Publisher
- American Society for Microbiology
- Published
- 1984-09
- Volume
- 159
- Issue
- 3
- Pages
- 811-819
- ISSN
- 0021-9193 (ISSN-L)
- 1098-5530
- OpenAlex
- W1950598798
- Cited by
- 277
- References
- 22
- FWCI
- 25.20
- Type
- journal article
- Language
- en
- Open access
- green
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- CoChat. (2026). Citation check: Genes for alkaline protease and neutral protease from Bacillus amyloliquefaciens contain a large open reading frame between the regions coding for signal sequence and mature protein. https://app.cochat.ai/papers/10.1128/jb.159.3.811-819.1984
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- Vasantha, N., Thompson, L. D., Rhodes, C., Banner, C., Nagle, J., & Filpula, D. (1984). Genes for alkaline protease and neutral protease from Bacillus amyloliquefaciens contain a large open reading frame between the regions coding for signal sequence and mature protein. Journal of Bacteriology. https://doi.org/10.1128/jb.159.3.811-819.1984
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