The DOI matches its Crossref record; 9 of the 9 references it cites were found in a registry or an index.
Buffer gradient gels and 35S label as an aid to rapid DNA sequence determination.
Open accessAbstract
Two methods for increasing the length of DNA sequence data that can be read off a polyacrylamide gel are described. We have developed a rapid way to pour a buffer concentration gradient gel that, by altering the vertical band separation on an autoradiograph, allows more sequence to be obtained from a gel. We also show that the use of deoxyadenosine 5'-(alpha-[35S]thio)triphosphate as the label incorporated in dideoxynucleotide sequence reactions increases the sharpness of the bands on an autoradiograph and so increases the resolution achieved.
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Cites
DNA sequencing with chain-terminating inhibitors
Sanger, F., Nicklen, S., & Coulson, A. R. (1977) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.74.12.5463 · Cited by 68,572
A new method for sequencing DNA.
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Cloning in single-stranded bacteriophage as an aid to rapid DNA sequencing
Sanger, F., Coulson, A. R., Barrell, B. G., Smith, A. J. H., & Roe, B. A. (1980) · Journal of Molecular Biology · doi:10.1016/0022-2836(80)90196-5 · Cited by 3,407
A new pair of M13 vectors for selecting either DNA strand of double-digest restriction fragments
Messing, J., & Vieira, J. (1982) · Gene · doi:10.1016/0378-1119(82)90016-6 · Cited by 2,896
The use of thin acrylamide gels for DNA sequencing
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Random subcloning of sonicated DNA: Application to shotgun DNA sequence analysis
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Improvements of DNA sequencing gels
Garoff, H., & Ansorge, W. (1981) · Analytical Biochemistry · doi:10.1016/0003-2697(81)90031-2 · Cited by 265
Brody, R. S., & Frey, P. A. (1981) · Biochemistry · doi:10.1021/bi00508a030 · Cited by 91
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Vosberg, H.-P., & Eckstein, F. (1977) · Biochemistry · doi:10.1021/bi00635a020 · Cited by 38
Cited by
Reanalysis and revision of the Cambridge reference sequence for human mitochondrial DNA
Andrews, R. M., Kubacka, I., Chinnery, P. F., Lightowlers, R. N., Turnbull, D. M., & Howell, N. (1999) · Nature Genetics · doi:10.1038/13779 · Cited by 3,337
Goedert, M., Spillantini, M. G., Jakes, R., Rutherford, D., & Crowther, R. A. (1989) · Neuron · doi:10.1016/0896-6273(89)90210-9 · Cited by 2,492
Nucleotide sequence of the AIDS virus, LAV
Wain-Hobson, S., Sonigo, P., Danos, O., Cole, S., & Alizon, M. (1985) · Cell · doi:10.1016/0092-8674(85)90303-4 · Cited by 1,202
Multiple Mutations in HIV-1 Reverse Transcriptase Confer High-Level Resistance to Zidovudine (AZT)
Larder, B. A., & Kemp, S. D. (1989) · Science · doi:10.1126/science.2479983 · Cited by 1,163
Goedert, M., Wischik, C. M., Crowther, R. A., Walker, J. E., & Klug, A. (1988) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.85.11.4051 · Cited by 1,134
Metadata
- Authors
- Biggin, M. D., Gibson, T. J., & Hong, G. F.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1983-07
- Volume
- 80
- Issue
- 13
- Pages
- 3963-3965
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2014689788
- Cited by
- 2,376
- References
- 9
- FWCI
- 94.14
- Type
- journal article
- Language
- en
- Open access
- green
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- Biggin, M. D., Gibson, T. J., & Hong, G. F. (1983). Buffer gradient gels and 35S label as an aid to rapid DNA sequence determination. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.80.13.3963
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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