The DOI matches its Crossref record; 17 of the 17 references it cites were found in a registry or an index.
Purification and characterization of a novel type of fimbriae from the oral anaerobe Bacteroides gingivalis
Open accessAbstract
Fimbriae and their constituent protein (fimbrilin) were purified to homogeneity from the bacterial wash fluid and cell lysate fraction, respectively, of Bacteroides gingivalis 381. Fimbriae, observed by negative staining, were curly, single-stranded filaments with a diameter of ca. 5 nm. The apparent molecular weight of the fimbrilin was 43,000. Fimbriae were resistant to sodium dodecyl sulfate denaturation at 70 degrees C. Heating at 100 degrees C in sodium dodecyl sulfate was needed to completely dissociate them to monomers of fimbrilin. Different sets of antigenic determinants seemed to be exposed on the surfaces of fimbriae and sodium dodecyl sulfate-denatured fimbrilin. Purified fimbriae did not show either hemagglutinating activity or hemagglutination inhibitory activity, although it has been inferred on the basis of circumstantial evidence that fimbriae are correlated to hemagglutinating activity of the organism. Hemagglutinin activity, however, was detected in culture supernatant, and this observation suggests that fimbriae of a different type or a lectin-like protein may be acting as hemagglutinin in B. gingivalis.
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Microbial interactions in building of communities
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Construction and characterization of a fimA mutant of Porphyromonas gingivalis
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Metadata
- Authors
- Yoshimura, F., Takahashi, K., Nodasaka, Y., & Suzuki, T.
- Venue
- Journal of Bacteriology
- Publisher
- American Society for Microbiology
- Published
- 1984-12
- Volume
- 160
- Issue
- 3
- Pages
- 949-957
- ISSN
- 0021-9193 (ISSN-L)
- 1098-5530
- OpenAlex
- W2122456003
- Cited by
- 299
- References
- 17
- FWCI
- 7.90
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Yoshimura, F., Takahashi, K., Nodasaka, Y., & Suzuki, T. (1984). Purification and characterization of a novel type of fimbriae from the oral anaerobe Bacteroides gingivalis. Journal of Bacteriology. https://doi.org/10.1128/jb.160.3.949-957.1984
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