Construction and characterization of a fimA mutant of Porphyromonas gingivalis

Hamada, N; Watanabe, K; Sasakawa, C; Yoshikawa, M; Yoshimura, F; Umemoto, T

doi:10.1128/iai.62.5.1696-1704.1994

Cited by 124Oral microbiology and periodontitis researchBiochemical and Structural CharacterizationMicrobial infections and disease research

Construction and characterization of a fimA mutant of Porphyromonas gingivalis

Open access

Hamada, N, Watanabe, K, Sasakawa, C, Yoshikawa, M, Yoshimura, F, Umemoto, T·1994·Infection and Immunity

DOI 10.1128/iai.62.5.1696-1704.1994PMC PMC186386

Open this paper in CoChat — free account

Abstract

Although fimbriae of Porphyromonas gingivalis have been implicated as playing a major role in adherence to gingival tissue surfaces, no conclusive genetic evidence has yet been obtained. The fimA gene, the determinant for the major fimbrial subunit protein, was cloned and sequenced (D. P. Dickinson, M. A. Kubiniec, F. Yoshimura, and R. J. Genco, J. Bacteriol. 170:1658-1665, 1988). We undertook to inactivate the fimA gene by a homologous recombination technique and examined the fimA mutant for changes in surface properties, including production of fimbriae, adherence to human gingival fibroblasts and epithelial cells, hemagglutinating activity, and surface hydrophobicity. To inactivate the fimA gene, we disrupted a fimA clone by insertion of a DNA segment containing an erythromycin resistance (Emr) gene. This was then delivered into P. gingivalis ATCC 33277 from an Escherichia coli K-12 strain, SM10 lambda pir, by using a mobilizable suicide vector, pGP704; recombination at the fimA locus led to the isolation of a fimA mutant. Disruption of the fimA locus and disappearance of FimA production were confirmed by Southern hybridization with a fimA-specific DNA probe and Western immunoblotting with a monoclonal antibody against the FimA protein, respectively. The fimA mutant constructed failed to express long (0.5- to 1.0-micron) fimbriae from the bacterial surface and had a diminished adhesive capacity to tissue-cultured human gingival fibroblasts and epithelial cells. Observation of the bacteria adhering to human gingival fibroblasts by scanning electron microscopy revealed that the wild-type strain had dramatic local changes in the appearance of the microvilli at the point of contact with large bacterial clumps, whereas the fimA mutant did not. In contrast, neither the hemagglutinating activity nor the surface hydrophobicity was changed in the fimA mutant. These data thus constitute the first direct genetic evidence demonstrating that the FimA protein of P. gingivalis is essential for the interaction of the organism with human gingival tissue cells through a function(s) encoded by the fimA gene.

Integrity signals

Registry

Crossref · 10.1128/iai.62.5.1696-1704.1994

Cites

45 of 45 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

We couldn’t retrieve this paper’s full text, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Cell surface hydrophobicity is another key factor contributing to bacterial biofilm formation, including oral bacteria such as P. gingivalis and F. nucleatum.
“Cell surface hydrophobicity is another key factor contributing to bacterial biofilm formation, including oral bacteria such as P. gingivalis and F. nucleatum [51,52].”
From Analysis of Chemical Structure and Antibiofilm Properties of Exopolysaccharides from Lactiplantibacillus plantarum EIR/IF-1 Postbiotics (2022) · cites this paper as [51]

A rapid alkaline extraction procedure for screening recombinant plasmid DNA
Birnboim, H. C., & Doly, J. (1979) · Nucleic Acids Research · doi:10.1093/nar/7.6.1513 · Cited by 14,686 · see citing works
A procedure for the isolation of deoxyribonucleic acid from micro-organisms
Marmur, J. (1961) · Journal of Molecular Biology · doi:10.1016/s0022-2836(61)80047-8 · Cited by 11,597 · see citing works
“Western Blotting”: Electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A
Burnette, W. N. (1981) · Analytical Biochemistry · doi:10.1016/0003-2697(81)90281-5 · Cited by 8,432 · see citing works
A novel suicide vector and its use in construction of insertion mutations: osmoregulation of outer membrane proteins and virulence determinants in Vibrio cholerae requires toxR
Miller, V. L., & Mekalanos, J. J. (1988) · Journal of Bacteriology · doi:10.1128/jb.170.6.2575-2583.1988 · Cited by 2,138 · see citing works
Molecular Weight Determination of Protein-Dodecyl Sulfate Complexes by Gel Electrophoresis in a Discontinuous Buffer System
Neville, D. M. (1971) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)61792-2 · Cited by 1,988 · see citing works
Microbial Pathogenicity Black-pigmented Bacteroides species, Capnocytophaga species, and Actinobacillus actinomycetemcomitans in Human Periodontal Disease: Virulence Factors in Colonization, Survival, and Tissue Destruction
Slots, J., & Genco, R. J. (1984) · Journal of Dental Research · doi:10.1177/00220345840630031101 · Cited by 939 · see citing works
A study of the bacteria associated with advancing periodontitis in man
Tanner, A. C. R., Haffer, C., Bratthall, G., Visconti, R. A., & Socransky, S. S. (1979) · Journal Of Clinical Periodontology · doi:10.1111/j.1600-051x.1979.tb01931.x · Cited by 918 · see citing works
Bacteroides gingivalis, Bacteroides intermedius and Actinobacillus actinomycetemcomitans in human periodontal diseases
Slots, J., & Listgarten, M. A. (1988) · Journal Of Clinical Periodontology · doi:10.1111/j.1600-051x.1988.tb00999.x · Cited by 711 · see citing works
Relationship of Bacteria to the Etiology of Periodontal Disease
Socransky, S. S. (1970) · Journal of Dental Research · doi:10.1177/00220345700490020401 · Cited by 621 · see citing works
Implantation of Bacteroides gingivalis in Nonhuman Primates Initiates Progression of Periodontitis
Holt, S. C., Ebersole, J. L., Felton, J. R., Brunsvold, M. A., & Kornman, K. S. (1988) · Science · doi:10.1126/science.3336774 · Cited by 464 · see citing works
Attachment of Bacteroides melaninogenicus subsp. asaccharolyticus to Oral Surfaces and Its Possible Role in Colonization of the Mouth and of Periodontal Pockets
Slots, J., & Gibbons, R. J. (1978) · Infection and Immunity · doi:10.1128/iai.19.1.254-264.1978 · Cited by 362 · see citing works
Functional characterization of extracellular vesicles produced by Bacteroides gingivalis
Grenier, D., & Mayrand, D. (1987) · Infection and Immunity · doi:10.1128/iai.55.1.111-117.1987 · Cited by 352 · see citing works
Proposal for Reclassification of Bacteroides asaccharolyticus, Bacteroides gingivalis, and Bacteroides endodontalis in a New Genus, Porphyromonas
Shah, H. N., & Collins, M. D. (1988) · International Journal of Systematic Bacteriology · doi:10.1099/00207713-38-1-128 · Cited by 349 · see citing works
Black-pigmented Bacteroides spp. in the human oral cavity
Zambon, J. J., Reynolds, H. S., & Slots, J. (1981) · Infection and Immunity · doi:10.1128/iai.32.1.198-203.1981 · Cited by 335 · see citing works
Purification and characterization of a novel type of fimbriae from the oral anaerobe Bacteroides gingivalis
Yoshimura, F., Takahashi, K., Nodasaka, Y., & Suzuki, T. (1984) · Journal of Bacteriology · doi:10.1128/jb.160.3.949-957.1984 · Cited by 300 · see citing works
Bacteriology of Human Experimental Gingivitis: Effect of Plaque and Gingivitis Score
Loesche, W. J., & Syed, S. A. (1978) · Infection and Immunity · doi:10.1128/iai.21.3.830-839.1978 · Cited by 244 · see citing works
Association of oral Bacteroides with gingivitis and adult periodontitis
White, D., & Mayrand, D. (1981) · Journal of Periodontal Research · doi:10.1111/j.1600-0765.1981.tb00974.x · Cited by 241 · see citing works
Interactions of Porphyromonas gingivalis with epithelial cells
Duncan, M. J., Nakao, S., Skobe, Z., & Xie, H. (1993) · Infection and Immunity · doi:10.1128/iai.61.5.2260-2265.1993 · Cited by 197 · see citing works
Selected characteristics of pathogenic and nonpathogenic strains of Bacteroides gingivalis
Grenier, D., & Mayrand, D. (1987) · Journal of Clinical Microbiology · doi:10.1128/jcm.25.4.738-740.1987 · Cited by 196 · see citing works
Molecular cloning and sequencing of the gene encoding the fimbrial subunit protein of Bacteroides gingivalis
Dickinson, D. P., Kubiniec, M. A., Yoshimura, F., & Genco, R. J. (1988) · Journal of Bacteriology · doi:10.1128/jb.170.4.1658-1665.1988 · Cited by 193 · see citing works
Bacteroides gingivalis, Bacteroides asaccharolyticus, andBacteroides melaninogenicus subspecies: Cell surface morphology and adherence to erythrocytes and human buccal epithelial cells
Okuda, K., Slots, J., & Genco, R. J. (1981) · Current Microbiology · doi:10.1007/bf01566718 · Cited by 187 · see citing works
Biochemical and immunobiological properties of lipopolysaccharide (LPS) from Bacteroides gingivalis and comparison with LPS from Escherichia coli
Koga, T., Nishihara, T., Fujiwara, T., Nisizawa, T., Okahashi, N., Noguchi, T., & Hamada, S. (1985) · Infection and Immunity · doi:10.1128/iai.47.3.638-647.1985 · Cited by 180 · see citing works
Specific inhibition of adherence of an oral strain of Bacteroides gingivalis 381 to epithelial cells by monoclonal antibodies against the bacterial fimbriae
Isogai, H., Isogai, E., Yoshimura, F., Suzuki, T., Kagota, W., & Takano, K. (1988) · Archives of Oral Biology · doi:10.1016/0003-9969(88)90028-3 · Cited by 167 · see citing works
Comparative hydrophobicities of oral bacteria and their adherence to salivary pellicles
Gibbons, R. J., & Etherden, I. (1983) · Infection and Immunity · doi:10.1128/iai.41.3.1190-1196.1983 · Cited by 165 · see citing works
Virulence of black‐pigmented Bacteroides strains from periodontal pockets and other sites in experimentally induced skin lesions in mice
van Steenbergen, T. J. M., Kastelein, P., Touw, J. J. A., & Graaff, J. (1982) · Journal of Periodontal Research · doi:10.1111/j.1600-0765.1982.tb01129.x · Cited by 163 · see citing works
Exological relationships of bacteria involved in a simple, mixed anaerobic infection
Mayrand, D., & McBride, B. C. (1980) · Infection and Immunity · doi:10.1128/iai.27.1.44-50.1980 · Cited by 152 · see citing works
Characterization of a trypsin-like protease from the bacterium Bacteroides gingivalis isolated from human dental plaque
Yoshimura, F., Nishikata, M., Suzuki, T., Hoover, C. I., & Newbrun, E. (1984) · Archives of Oral Biology · doi:10.1016/0003-9969(84)90078-5 · Cited by 152 · see citing works
Lipoteichoic acid is the major cell wall component responsible for surface hydrophobicity of group A streptococci
Miörner, H., Johansson, G., & Kronvall, G. (1983) · Infection and Immunity · doi:10.1128/iai.39.1.336-343.1983 · Cited by 125 · see citing works
Purification and properties of hemagglutinin from culture supernatant of Bacteroides gingivalis
Okuda, K., Yamamoto, A., Naito, Y., TAKAZOE, I., Slots, J., & Genco, R. J. (1986) · Infection and Immunity · doi:10.1128/iai.54.3.659-665.1986 · Cited by 119 · see citing works
Adherence of Acinetobacter calcoaceticus RAG-1 to human epithelial cells and to hexadecane
Rosenberg, M., Perry, A., Bayer, E. A., Gutnick, D. L., ROSENBERG, E. B., & Ofek, I. (1981) · Infection and Immunity · doi:10.1128/iai.33.1.29-33.1981 · Cited by 110 · see citing works
Evidence that the clindamycin-erythromycin resistance gene of Bacteroides plasmid pBF4 is on a transposable element
Shoemaker, N. B., Guthrie, E. P., Salyers, A. A., & Gardner, J. F. (1985) · Journal of Bacteriology · doi:10.1128/jb.162.2.626-632.1985 · Cited by 108 · see citing works
Evidence that Porphyromonas (Bacteroides) gingivalis fimbriae function in adhesion to Actinomyces viscosus
Goulbourne, P. A., & Ellen, R. P. (1991) · Journal of Bacteriology · doi:10.1128/jb.173.17.5266-5274.1991 · Cited by 108 · see citing works
Bacteroides gingivalis fimbriae stimulate production of thymocyte-activating factor by human gingival fibroblasts
Hanazawa, S., Hirose, K., Ohmori, Y., Amano, S., & Kitano, S. (1988) · Infection and Immunity · doi:10.1128/iai.56.1.272-274.1988 · Cited by 107 · see citing works
Isolation and some properties of exohemagglutinin from the culture medium of Bacteroides gingivalis 381
INOSHITA, E., Amano, A., Hanioka, T., Tamagawa, H., Shizukuishi, S., & Tsunemitsu, A. (1986) · Infection and Immunity · doi:10.1128/iai.52.2.421-427.1986 · Cited by 98 · see citing works
Attachment of Bacteroides gingivalis to Collagenous Substrata
Naito, Y., & Gibbons, R. J. (1988) · Journal of Dental Research · doi:10.1177/00220345880670080301 · Cited by 89 · see citing works
Cell-surface hydrophobicity of adherent oral bacteria
Weiss, E. I., Rosenberg, M., Judes, H., & ROSENBERG, E. B. (1982) · Current Microbiology · doi:10.1007/bf01568427 · Cited by 86 · see citing works
Thiol‐dependent collagenolytic activity in culture media of Bacteroides gingivalis
Toda, K., Otsuka, M., Ishikawa, Y., Sato, M., and, Y. Y., & Nakamura, R. (1984) · Journal of Periodontal Research · doi:10.1111/j.1600-0765.1984.tb01010.x · Cited by 75 · see citing works
Correlation between cell‐adherent activity and surface structure in Porphyromonas gingivalis
Watanabe, K., Yamaji, Y., & Umemoto, T. (1992) · Oral Microbiology and Immunology · doi:10.1111/j.1399-302x.1992.tb00636.x · Cited by 73 · see citing works
Proteins with molecular masses of 50 and 80 kilodaltons encoded by genes downstream from the fimbrilin gene (fimA) are components associated with fimbriae in the oral anaerobe Porphyromonas gingivalis
Yoshimura, F., Takahashi, Y., Eiko, H. I. B. I., Takasawa, T., Kato, H., & Dickinson, D. P. (1993) · Infection and Immunity · doi:10.1128/iai.61.12.5181-5189.1993 · Cited by 56 · see citing works
Selective modulation of bacterial attachment to oral epithelial cells by enzyme activities associated with poor oral hygiene
Childs, W. C., & Gibbons, R. J. (1990) · Journal of Periodontal Research · doi:10.1111/j.1600-0765.1990.tb01040.x · Cited by 55 · see citing works
Surface appendages, hemagglutination, and adherence to human epithelial cells of Bacteroides intermedius
Leung, K. ‐., Fukushima, H., Sagawa, H., Walker, C., & CLARK, W. B. (1989) · Oral Microbiology and Immunology · doi:10.1111/j.1399-302x.1989.tb00253.x · Cited by 53 · see citing works
Restriction Fragment Length Polymorphism Analysis of the Fimbrillin Locus, fimA, of Porphyromonas gingivalis
Loos, B. G., & Dyer, D. W. (1992) · Journal of Dental Research · doi:10.1177/00220345920710050901 · Cited by 47 · see citing works
Alteration of fimbriation and cell hydrophobicity by sublethal concentrations of tetracycline
Peros, W. J., Etherden, I., Gibbons, R. J., & Skobe, Z. (1985) · Journal of Periodontal Research · doi:10.1111/j.1600-0765.1985.tb00406.x · Cited by 27 · see citing works
Detection of fimbrilin gene (fimA) in Porphyromonas (Bacteroides) gingivalis by Southern blot analysis
Takahashi, Y., Yoshimura, F., Kawanami, M., & Kato, H. (1992) · Journal of Periodontal Research · doi:10.1111/j.1600-0765.1992.tb01742.x · Cited by 26 · see citing works
Transfer of plasmid pE5‐2 from Escherichia coli to Bacteroides gingivalis and B. intermedius
Progulske‐Fox, A., Oberste, A. D., Drummond, C., & McArthur, W. P. (1989) · Oral Microbiology and Immunology · doi:10.1111/j.1399-302x.1989.tb00239.x · Cited by 23 · see citing works

Flagged first, then by citations

Sorted by citations

Life Below the Gum Line: Pathogenic Mechanisms ofPorphyromonas gingivalis
Lamont, R. J., & Jenkinson, H. F. (1998) · Microbiology and Molecular Biology Reviews · doi:10.1128/mmbr.62.4.1244-1263.1998 · Cited by 964 · see citing works
“naeslundii; and fibrinogen, fibronectin, and lactoferrin (7, 9, 76, 88, 105, 128, 136, 162, 252, 255, 274).”
Porphyromonas gingivalis: An Overview of Periodontopathic Pathogen below the Gum Line
How, K. Y., Song, K. P., & Chan, K. G. (2016) · Frontiers in Microbiology · doi:10.3389/fmicb.2016.00053 · Cited by 858 · see citing works
“It constitues an important component of the bacterial outer membrane (Hamada et al., 1994).”
Virulence factors of Porphyromonas gingivalis
Holt, S. C., Kesavalu, L. N., Walker, S. G., & Genco, C. A. (1999) · Periodontology 2000 · doi:10.1111/j.1600-0757.1999.tb00162.x · Cited by 644 · see citing works
Oral Mucosal Epithelial Cells
Groeger, S., & Meyle, J. (2019) · Frontiers in Immunology · doi:10.3389/fimmu.2019.00208 · Cited by 473 · see citing works
Generation of inflammatory stimuli: how bacteria set up inflammatory responses in the gingiva
Madianos, P. N., Bobetsis, Y. A., & Kinane, D. F. (2005) · Journal Of Clinical Periodontology · doi:10.1111/j.1600-051x.2005.00821.x · Cited by 310 · see citing works
Epithelial barrier and oral bacterial infection
Groeger, S. E., & Meyle, J. (2015) · Periodontology 2000 · doi:10.1111/prd.12094 · Cited by 229 · see citing works
Pathogenic strategies of the oral anaerobe, Porphyromonas gingivalis
Cutler, C. W., Kalmar, J. R., & Genco, C. A. (1995) · Trends in Microbiology · doi:10.1016/s0966-842x(00)88874-5 · Cited by 214 · see citing works
Role of fimbriae in Porphyromonas gingivalis invasion of gingival epithelial cells
Weinberg, A., Belton, C. M., Park, Y., & Lamont, R. J. (1997) · Infection and Immunity · doi:10.1128/iai.65.1.313-316.1997 · Cited by 208 · see citing works
“(13) which demonstrated that adherence of a fimbria-deficient mutant of P.”
Background
A role for fimbriae in Porphyromonas gingivalis invasion of oral epithelial cells
Njoroge, T., Genco, R. J., Sojar, H. T., Hamada, N., & Genco, C. A. (1997) · Infection and Immunity · doi:10.1128/iai.65.5.1980-1984.1997 · Cited by 201 · see citing works
“For scanning electron microscopy, human gingival epithelial layers (Ca9-22) (11) cultured on round glass slides were incubated for 60 min with P.”
BackgroundMethodInfluential
The importance of fimbriae in the virulence and ecology of some oral bacteria
Hamada, S., Amano, A., Kimura, S., Nakagawa, I., Kawabata, S., & Morisaki, I. (1998) · Oral Microbiology and Immunology · doi:10.1111/j.1399-302x.1998.tb00724.x · Cited by 161 · see citing works
Involvement of arginine-specific cysteine proteinase (Arg-gingipain) in fimbriation of Porphyromonas gingivalis
Nakayama, K., Yoshimura, F., Kadowaki, T., & Yamamoto, K. (1996) · Journal of Bacteriology · doi:10.1128/jb.178.10.2818-2824.1996 · Cited by 147 · see citing works
“(6) and saliva-coated hydroxyapatite (12).”
Background
Porphyromonas gingivalis induces murine macrophage foam cell formation
Qi, M., Miyakawa, H., & Kuramitsu, H. K. (2003) · Microbial Pathogenesis · doi:10.1016/j.micpath.2003.07.002 · Cited by 142 · see citing works
B7-H1 and B7-DC receptors of oral squamous carcinoma cells are upregulated by Porphyromonas gingivalis
Groeger, S. E., Domann, E., Gonzales, J. R., Chakraborty, T., & Meyle, J. (2011) · Immunobiology · doi:10.1016/j.imbio.2011.05.005 · Cited by 131 · see citing works
Oral pathogens: from dental plaque to cardiac disease
Meyer, D. H., & Fives-Taylor, P. M. (1998) · Current Opinion in Microbiology · doi:10.1016/s1369-5274(98)80147-1 · Cited by 129 · see citing works
Isolation and characterization of a minor fimbria from Porphyromonas gingivalis
Hamada, N., Sojar, H. T., Cho, M. I., & Genco, R. J. (1996) · Infection and Immunity · doi:10.1128/iai.64.11.4788-4794.1996 · Cited by 120 · see citing works
“gingivalis ATCC 33277 and a fimA mutant, MPG1 (7).”
BackgroundInfluential
Distinct roles of long/short fimbriae and gingipains in homotypic biofilm development by Porphyromonas gingivalis
Kuboniwa, M., Amano, A., Hashino, E., Yamamoto, Y., Inaba, H., Hamada, N., Nakayama, K., Tribble, G. D., Lamont, R. J., & Shizukuishi, S. (2009) · BMC Microbiology · doi:10.1186/1471-2180-9-105 · Cited by 115 · see citing works
“In addition, the hydrophobicity was reported not to depend on the presence or absence of FimA on the bacterial surface [39].”
Antigens of bacteria associated with periodontitis
O’brien-simpson, N. M., Veith, P. D., Dashper, S. G., & Reynolds, E. C. (2004) · Periodontology 2000 · doi:10.1111/j.0906-6713.2004.003559.x · Cited by 114 · see citing works
Induction of the immune response to periodontopathic bacteria and its role in the pathogenesis of periodontitis
Ishikawa, I., Nakashima, K., Koseki, T., Nagasawa, T., Watanabe, H., Arakawa, S., Nitta, H., & Nishihara, T. (1997) · Periodontology 2000 · doi:10.1111/j.1600-0757.1997.tb00193.x · Cited by 113 · see citing works
Protease-Activated Receptor Signaling Increases Epithelial Antimicrobial Peptide Expression
Chung, W. O., Hansen, S. R., Rao, D. K., & Dale, B. A. (2004) · The Journal of Immunology · doi:10.4049/jimmunol.173.8.5165 · Cited by 109 · see citing works
Environmental regulation of fimbrial gene expression in Porphyromonas gingivalis
Xie, H., Cai, S., & Lamont, R. J. (1997) · Infection and Immunity · doi:10.1128/iai.65.6.2265-2271.1997 · Cited by 108 · see citing works
“gingivalis to saliva-coated hydroxyapatite beads, other oral bacteria, human gingival fibroblasts, and epithelial cells (3, 14, 18, 21).”
Background
Fimbria-dependent activation of pro-inflammatory molecules in Porphyromonas gingivalis infected human aortic endothelial cells
Takahashi, Y., Davey, M., Yumoto, H., Gibson, F. C., & Genco, C. A. (2005) · Cellular Microbiology · doi:10.1111/j.1462-5822.2005.00661.x · Cited by 105 · see citing works
Periodontal disease: bacterial virulence factors, host response and impact on systemic health
Graves, D. T., Jiang, Y., & Genco, C. A. (2000) · Current Opinion in Infectious Diseases · doi:10.1097/00001432-200006000-00005 · Cited by 103 · see citing works
Distribution of fimA genotypes of Porphyromonas gingivalis in subjects with various periodontal conditions
Missailidis, C. G., Umeda, J. E., Ota‐Tsuzuki, C., Anzai, D., & Mayer, M. P. A. (2004) · Oral Microbiology and Immunology · doi:10.1111/j.1399-302x.2004.00140.x · Cited by 101 · see citing works
FimbriatedPorphyromonas gingivalisIs More Efficient than Fimbria-DeficientP. gingivalisin Entering Human Dendritic Cells In Vitro and Induces an Inflammatory Th1 Effector Response
Jotwani, R., & Cutler, C. W. (2004) · Infection and Immunity · doi:10.1128/iai.72.3.1725-1732.2004 · Cited by 101 · see citing works
“gingivalis into other host cells (13, 22, 32, 33, 50, 54), we postulated that entry into MDDCs would also be fimbria dependent.”
Background
Local and Systemic Effects of Porphyromonas gingivalis Infection
Chen, W. A., Dou, Y., Fletcher, H. M., & Boskovic, D. S. (2023) · Microorganisms · doi:10.3390/microorganisms11020470 · Cited by 102 · see citing works
“gingivalis strains deficient in long fimbriae [71].”
A Peptide Domain on Gingipain R Which Confers Immunity against Porphyromonas gingivalis Infection in Mice
Genco, C. A., Odusanya, B. M., Potempa, J., Mikolajczyk‐Pawlinska, J., & Travis, J. W. (1998) · Infection and Immunity · doi:10.1128/iai.66.9.4108-4114.1998 · Cited by 86 · see citing works
Invasion Strategies of the Oral Pathogen Porphyromonas gingivalis: Implications for Cardiovascular Disease
Deshpande, R. G., Khan, M. B., & Genco, C. A. (1998) · Invasion and Metastasis · doi:10.1159/000024499 · Cited by 84 · see citing works
Structural domains of Porphyromonas gingivalis recombinant fimbrillin that mediate binding to salivary proline-rich protein and statherin
Amano, A., Sharma, A., Lee, J. Y., Sojar, H. T., Raj, P. A., & Genco, R. J. (1996) · Infection and Immunity · doi:10.1128/iai.64.5.1631-1637.1996 · Cited by 81 · see citing works
“The fimbria-deficient mutant was also unable to bind to human gingival fibroblasts and epithelial cells (10).”
Background
Interrupting oral infection of Porphyromonas gingivalis with anti-FimA antibody attenuates bacterial dissemination to the arthritic joint and improves experimental arthritis
Jeong, S. H., Nam, Y., Jung, H., Kim, J., Rim, Y. A., Park, N., Lee, K.-J., Choi, S., Jang, Y., Kim, Y., et al. (2018) · Experimental & Molecular Medicine · doi:10.1038/emm.2017.301 · Cited by 78 · see citing works
M olecular G enetic A nalysis of the V irulence of O ral B acterial P athogens: A n H istorical P erspective
Kuramitsu, H. K. (2003) · Critical Reviews in Oral Biology & Medicine · doi:10.1177/154411130301400504 · Cited by 78 · see citing works
Role of Porphyromonas gingivalis protease activity in colonization of oral surfaces
Tokuda, M., Duncan, M., Cho, M. I., & Kuramitsu, H. K. (1996) · Infection and Immunity · doi:10.1128/iai.64.10.4067-4073.1996 · Cited by 77 · see citing works
“gingivalis 33277 exhibited weak binding to fibroblast and epithelial cells (13).”
Background
Bacterial Fimbriae Stimulate Proinflammatory Activation in the Endothelium through Distinct TLRs
Davey, M., Liu, X., Ukai, T., Jain, V., Gudino, C. V., Gibson, F. C., Golenbock, D. T., Visintin, A., & Genco, C. A. (2008) · The Journal of Immunology · doi:10.4049/jimmunol.180.4.2187 · Cited by 76 · see citing works
“fimbriae expressed simultaneously by this organism (14, 15).”
Background
Invasion of Porphyromonas gingivalis strains into vascular cells and tissue
Olsen, I., & Progulske‐Fox, A. (2015) · Journal of Oral Microbiology · doi:10.3402/jom.v7.28788 · Cited by 75 · see citing works
“In addition, it has been reported that non-fimbriated strains have a reduced capacity to invade host cells (15, 18, 19).”
Background
New approaches to combat Porphyromonas gingivalis biofilms
Gerits, E., Verstraeten, N., & Michiels, J. J. (2017) · Journal of Oral Microbiology · doi:10.1080/20002297.2017.1300366 · Cited by 76 · see citing works
“fibroblasts and epithelial cells, demonstrating that FimA fimbriae play a role in the initial attachment of bacteria to host cells [15,16].”
Background
Role of Arg-Gingipain A in Virulence of Porphyromonas gingivalis
Tokuda, M., Karunakaran, T., Duncan, M. J., Hamada, N., & Kuramitsu, H. K. (1998) · Infection and Immunity · doi:10.1128/iai.66.3.1159-1166.1998 · Cited by 74 · see citing works
“binding to epithelial cells (8, 27), it appears to be difficult to”
Background
Domain‐Specific Rearrangement between the Two Arg‐Gingipain‐Encoding Genes in Porphyromonas gingivalis: Possible Involvement of Nonreciprocal Recombination
Nakayama, K. (1997) · Microbiology and Immunology · doi:10.1111/j.1348-0421.1997.tb01189.x · Cited by 74 · see citing works
Negative Correlation of Distributions of Streptococcus cristatus and Porphyromonas gingivalis in Subgingival Plaque
Wang, B.-Y., Wu, J., Lamont, R. J., Lin, X., & Xie, H. (2009) · Journal of Clinical Microbiology · doi:10.1128/jcm.00072-09 · Cited by 72 · see citing works
“gingivalis strain with a fimA deficiency has a diminished capacity to adhere to human gingival fibroblasts and epithelial cells (6) and is deficient in invasion of epithelial cells (35).”
Background
Porphyromonas gingivalis Minor Fimbriae Are Required for Cell-Cell Interactions
Lin, X., Wu, J., & Xie, H. (2006) · Infection and Immunity · doi:10.1128/iai.00797-06 · Cited by 68 · see citing works
“A deficiency in the fimA gene leads to a diminished capacity to adhere to human gingival fibroblasts and epithelial cells (5) and attenuation of periodontal bone loss in a gnotobiotic-rat model (10).”
Background
Characterization of Biologically Active Cell Surface Components of a Periodontal Pathogen. The Roles of Major and Minor Fimbriae of Porphyromonas gingivalis
Umemoto, T., & Hamada, N. (2003) · Journal of Periodontology · doi:10.1902/jop.2003.74.1.119 · Cited by 68 · see citing works
Effects of Porphyromonas gingivalis infection on human gingival epithelial barrier function in vitro
Groeger, S. E., Doman, E., Chakraborty, T., & Meyle, J. (2010) · European Journal Of Oral Sciences · doi:10.1111/j.1600-0722.2010.00782.x · Cited by 66 · see citing works
Porphyromonas gingivalis Fimbriae Bind to Cytokeratin of Epithelial Cells
Sojar, H. T., Sharma, A., & Genco, R. J. (2002) · Infection and Immunity · doi:10.1128/iai.70.1.96-101.2002 · Cited by 64 · see citing works
“gingivalis 33277 (MPG1) lacking fimbriae does not adhere to gingival fibroblasts or epithelial cells (6), strongly implicating fimbriae in adherence to these mammalian cells.”
Background
Porphyromonas gingivalis proteinases in periodontitis, a review.
Potempa, J., & Travis, J. W. (1996) · Acta Biochimica Polonica · doi:10.18388/abp.1996_4477 · Cited by 62 · see citing works
Adhesins encoded by the gingipain genes of Porphyromonas gingivalis are responsible for co-aggregation with Prevotella intermedia
Kamaguchi, A., Ohyama, T., Sakai, E., Nakamura, R. M., Watanabe, T., Baba, H., & Nakayama, K. (2003) · Microbiology · doi:10.1099/mic.0.25997-0 · Cited by 60 · see citing works
“The initial step of Por. gingivalis attachment to the oral tissue has been shown to be fimbriae-mediated (Hamada et al., 1994; Sojar et al., 1999, 2002).”
Background
Porphyromonas gingivalis67-kDa fimbriae induced cytokine production and osteoclast differentiation utilizing TLR2
Hiramine, H., Watanabe, K., Hamada, N., & Umemoto, T. (2003) · FEMS Microbiology Letters · doi:10.1016/s0378-1097(03)00788-2 · Cited by 57 · see citing works
“gingivalis possesses two distinctly di¡erent types of ¢mbriae in terms of their size and antigenicity on the cell surface [2,3], namely a 41-kDa protein (FimA) and a 67kDa protein (Mfa1) in strain ATCC 33277 [4,5].”
Background
Both the unique and repeat regions of the Porphyromonas gingivalis hemagglutin A are involved in adhesion and invasion of host cells
Bélanger, M., Kozarov, E. V., Song, H., Whitlock, J. A., & Progulske‐Fox, A. (2011) · Anaerobe · doi:10.1016/j.anaerobe.2011.10.005 · Cited by 52 · see citing works
“gingivalis to gingival epithelial cells [22, 25].”
Porphyromonas gingivalis Fimbriae Use β2Integrin (CD11/CD18) on Mouse Peritoneal Macrophages as a Cellular Receptor, and the CD18 β Chain Plays a Functional Role in Fimbrial Signaling
Takeshita, A., Murakami, Y., Yamashita, Y., Ishida, M., Fujisawa, S., Kitano, S., & Hanazawa, S. (1998) · Infection and Immunity · doi:10.1128/.66.9.4056-4060.1998 · Cited by 52 · see citing works
Binding sites of salivary statherin for Porphyromonas gingivalis recombinant fimbrillin
Amano, A., Kataoka, K., Raj, P. A., Genco, R. J., & Shizukuishi, S. (1996) · Infection and Immunity · doi:10.1128/iai.64.10.4249-4254.1996 · Cited by 51 · see citing works
“gingivalis mutant with an inactivated fimA gene cannot bind to human gingival fibroblasts, epithelial cells (9), and whole-saliva-coated hydroxyapatite (HAP) beads (14).”
Background
Genetic Manipulation of Porphyromonas gingivalis
Bélanger, M., Rodrigues, P. H. M., & Progulske‐Fox, A. (2007) · Current Protocols in Microbiology · doi:10.1002/9780471729259.mc13c02s05 · Cited by 49 · see citing works
“In 1994, several reports were published describing mutant constructions for other genes using suicide vectors (Joe et al., 1994; Hamada et al., 1994; Malek et al., 1994; Nakayama, 1994).”
BackgroundMethod
FimB Regulates FimA Fimbriation in Porphyromonas gingivalis
Nagano, K., Hasegawa, Y., Murakami, Y., Nishiyama, S., & Yoshimura, F. (2010) · Journal of Dental Research · doi:10.1177/0022034510370089 · Cited by 47 · see citing works
“Indeed, the often-used strains ATCC 33277 (33277) (Hamada et al., 1994) and 381 (Yoshimura et al.”
Method
Molecular and antigenic similarities of the fimbrial major components between Porphyromonas gulae and P. gingivalis
Hamada, N., Takahashi, Y., Watanabe, K., Kumada, H., Oishi, Y., & Umemoto, T. (2007) · Veterinary Microbiology · doi:10.1016/j.vetmic.2007.09.014 · Cited by 43 · see citing works
Porphyromonas gingivalis fimbrillin is one of the fibronectin-binding proteins
Murakami, Y., Iwahashi, H., Yasuda, H., Umemoto, T., Namikawa, I., Kitano, S., & Hanazawa, S. (1996) · Infection and Immunity · doi:10.1128/iai.64.7.2571-2576.1996 · Cited by 43 · see citing works
“Our suggestion has been confirmed genetically by a recent study showing that a mutation of the fimA gene encoding the major subunit of the fimbriae, fimbrillin, prevents bacterial adherence to host cells (6).”
Result
OxyR is involved in coordinate regulation of expression of fimA and sod genes in Porphyromonas gingivalis
Wu, J., Lin, X., & Xie, H. (2008) · FEMS Microbiology Letters · doi:10.1111/j.1574-6968.2008.01116.x · Cited by 42 · see citing works
“A P. gingivalis strain with fimA deficiency has a diminished capacity to adhere to human gingival fibroblasts and epithelial cells (Hamada et al., 1994).”
Background
Construction and preliminary characterization of three hemagglutinin mutants of Porphyromonas gingivalis
Lépine, G., Ellen, R. P., & Progulske‐Fox, A. (1996) · Infection and Immunity · doi:10.1128/iai.64.4.1467-1472.1996 · Cited by 42 · see citing works
“gingivalis 381 (8), insertional interruption of the hag genes was not accompanied by a noticeable change in the hydrophobicity of the mutants.”
Background
Molecular and Immunological Characterization of the Fimbriae of Porphyromonas gingivalis
Hamada, S., Fujiwara, T., Morishima, S., Takahashi, I., Nakagawa, I., Kimura, S., & Ogawa, T. (1994) · Microbiology and Immunology · doi:10.1111/j.1348-0421.1994.tb02148.x · Cited by 42 · see citing works
Role of the Amino-Terminal Region of Porphyromonas gingivalis Fimbriae in Adherence to Epithelial Cells
Sojar, H. T., Han, Y. W., Hamada, N., Sharma, A., & Genco, R. J. (1999) · Infection and Immunity · doi:10.1128/iai.67.11.6173-6176.1999 · Cited by 40 · see citing works
“Recent reports from several laboratories have shown that fimbriae bind to saliva-coated hydroxyapatite (13), human erythrocytes (17, 18), monocytes and macrophages (19), epithelial cells (7, 11, 16, 30), and gingival fibroblasts (8).”
Background
Histidine Kinase-Mediated Production and Autoassembly of Porphyromonas gingivalis Fimbriae
Nishikawa, K., & Duncan, M. J. (2010) · Journal of Bacteriology · doi:10.1128/jb.01474-09 · Cited by 40 · see citing works
Development of a Gene Inactivation System for Bacteroides forsythus : Construction and Characterization of a BspA Mutant
Honma, K., Kuramitsu, H. K., Genco, R. J., & Sharma, A. (2001) · Infection and Immunity · doi:10.1128/iai.69.7.4686-4690.2001 · Cited by 40 · see citing works
“gingivalis (8, 13) do not show any truncated FimA proteins.”
Background
Identification of Porphyromonas gingivalis prefimbrilin possessing a long leader peptide: possible involvement of trypsin-like protease in fimbrilin maturation
Onoe, T., Hoover, C. I., Nakayama, K., Ideka, T., Nakamura, H., & Yoshimura, F. (1995) · Microbial Pathogenesis · doi:10.1016/s0882-4010(96)80006-4 · Cited by 40 · see citing works
Cellular events concurrent with porphyromonas gingivalis invasion of oral epithelium in vitro
Sandros, J., Madianos, P. N., & Papapanou, P. N. (1996) · European Journal Of Oral Sciences · doi:10.1111/j.1600-0722.1996.tb00093.x · Cited by 39 · see citing works
An antiserum to a synthetic fimbrial peptide of Actinobacillus actinomycetemcomitans blocked adhesion of the microorganism
Harano, K., Yamanaka, A., & Okuda, K. (1995) · FEMS Microbiology Letters · doi:10.1016/0378-1097(95)00219-u · Cited by 37 · see citing works
Sequence and Product Analyses of the Four Genes Downstream from the Fimbrilin Gene (fimA) of the Oral Anaerobe Porphyromonas gingivalis
Watanabe, K., Onoe, T., Ozeki, M., Shimizu, Y., Sakayori, T., Nakamura, H., & Yoshimura, F. (1996) · Microbiology and Immunology · doi:10.1111/j.1348-0421.1996.tb01133.x · Cited by 37 · see citing works
Citrullination mediated by PPAD constrains biofilm formation in P. gingivalis strain 381
Vermilyea, D. M., Ottenberg, G. K., & Davey, M. E. (2019) · npj Biofilms and Microbiomes · doi:10.1038/s41522-019-0081-x · Cited by 35 · see citing works
Differential expression and adherence of Porphyromonas gingivalis FimA genotypes
Zheng, C., Wu, J., & Xie, H. (2011) · Molecular Oral Microbiology · doi:10.1111/j.2041-1014.2011.00626.x · Cited by 35 · see citing works
“gingivalis strains and also with previous reports (Hamada et al., 1994; Lin et al., 2006) supporting a critical role of FimA in promoting the attachment of P.”
Result
Identification of Escherichia coli Genes That Are Specifically Expressed in a Murine Model of Septicemic Infection
Khan, M. A., & Isaacson, R. E. (2002) · Infection and Immunity · doi:10.1128/iai.70.7.3404-3412.2002 · Cited by 35 · see citing works
“mutations were introduced into i484 as described previously (12).”
Method
Purification and characterization of a novel secondary fimbrial protein from Porphyromonas gingivalis strain 381
Arai, M., Hamada, N., & Umemoto, T. (2000) · FEMS Microbiology Letters · doi:10.1016/s0378-1097(00)00459-6 · Cited by 34 · see citing works
In Vitro Models That Support Adhesion Specificity in Biofilms of Oral Bacteria
Ellen, R. P., Lépine, G., & Nghiem, P.-M. (1997) · Advances in Dental Research · doi:10.1177/08959374970110011401 · Cited by 34 · see citing works
Immune response of macrophages from young and aged mice to the oral pathogenic bacterium Porphyromonas gingivalis
Shaik-Dasthagirisaheb, Y. B., Kantarcı, A., & Gibson, F. C. (2010) · Immunity & Ageing · doi:10.1186/1742-4933-7-15 · Cited by 32 · see citing works
“Focusing on chemokine expression, we observed that Pg DPG3 stimulated BMM also to produce MIP-1 a as compared with unchallenged control, like TNF-a , these levels were significantly less than that observed with wild type Pg challenge (Figure 2C).”
MethodInfluential
Cytokine production induced by a 67‐kDa fimbrial protein from Porphyromonas gingivalis
Hamada, N., Watanabe, K., Arai, M., Hiramine, H., & Umemoto, T. (2002) · Oral Microbiology and Immunology · doi:10.1034/j.1399-302x.2002.170311.x · Cited by 31 · see citing works
Isolation and characterization of transposon-induced mutants of deficient in fimbriation
Watanabe-Kato, T., Hayashi, J., Terazawa, Y., Hoover, C. I., Nakayama, K., Eiko, H. I. B. I., Kawakami, N., Ikeda, T., Nakamura, H., Noguchi, T., et al. (1998) · Microbial Pathogenesis · doi:10.1006/mpat.1997.0170 · Cited by 31 · see citing works
Identification of bistable populations of Porphyromonas gingivalis that differ in epithelial cell invasion
Suwannakul, S., Stafford, G. P., Whawell, S. A., & Douglas, C. W. I. (2010) · Microbiology · doi:10.1099/mic.0.038075-0 · Cited by 29 · see citing works
“However, there have also been reports that strains with identical fimbriation characteristics vary in their invasive ability (Dorn et al., 2000; Duncan et al., 1993; Hamada et al., 1994; Eick et al., 2006), indicating uncertainty about Abbreviations: AMC, 7-amido-4-methylcoumarin; B-Arg-AMC, aNbenzoyl-L-arginine-7-ami…”
Background
Identification of glyceraldehyde-3-phosphate dehydrogenase of epithelial cells as a second molecule that binds toPorphyromonas gingivalisfimbriae
Sojar, H. T., & Genco, R. J. (2005) · FEMS Immunology & Medical Microbiology · doi:10.1016/j.femsim.2005.01.006 · Cited by 29 · see citing works
“gingivalis have been shown to have diminished capacity for adherence to oral epithelial cells in in vitro assays [5].”
Background
Fimbriae and the hemagglutinating adhesin HA-Ag2 mediate adhesion of Porphyromonas gingivalis to epithelial cells
Dû, L. L., Pellen‐Mussi, P., Chandad, F., Mouton, C., & Bonnaure‐Mallet, M. (1997) · Infection and Immunity · doi:10.1128/iai.65.9.3875-3881.1997 · Cited by 28 · see citing works
“(11), who showed that the FimA protein of P.”
Background
Analysis of Chemical Structure and Antibiofilm Properties of Exopolysaccharides from Lactiplantibacillus plantarum EIR/IF-1 Postbiotics
Karaca, B., Haliscelik, O., Gursoy, M., Kiran, F., Loimaranta, V., Söderling, E., & Gursoy, U. K. (2022) · Microorganisms · doi:10.3390/microorganisms10112200 · Cited by 26 · see citing works
Hemin-binding protein 35 (HBP35) plays an important role in bacteria–mammalian cells interactions in Porphyromonas gingivalis
Hiratsuka, K., Kiyama-Kishikawa, M., & Abiko, Y. (2010) · Microbial Pathogenesis · doi:10.1016/j.micpath.2010.01.001 · Cited by 22 · see citing works
Porphyromonas gingivalis fimbriae are pro-inflammatory but do not play a prominent role in the innate immune response to P. gingivalis
Graves, D. T., Naguib, G. H., Lü, H., Desta, T., & Amar, S. (2005) · Journal of Endotoxin Research · doi:10.1177/09680519050110010501 · Cited by 21 · see citing works
Designing a peptide-based vaccine against Porphyromonas gingivalis
Lauritano, D. (2012) · Frontiers in Bioscience-Scholar · doi:10.2741/s395 · Cited by 19 · see citing works
Porphyromonas gingivalis fimbria-stimulated bone resorption in vitro is inhibited by a tyrosine kinase inhibitor
Hanazawa, S., Kawata, Y., Murakami, Y., Naganuma, K., Amano, S., Miyata, Y., & Kitano, S. (1995) · Infection and Immunity · doi:10.1128/iai.63.6.2374-2377.1995 · Cited by 19 · see citing works
Adhesion of Porphyromonas gingivalis Strains to Cultured Epithelial Cells From Patients With a History of Chronic Adult Periodontitis or From Patients Less Susceptible to Periodontitis
Quirynen, M., Papaioannou, W., van Steenbergen, T. J. M., Dierickx, K., Cassiman, J. J., & van Steenberghe, D. (2001) · Journal of Periodontology · doi:10.1902/jop.2001.72.5.626 · Cited by 18 · see citing works
Development of a novel plasmid vector pTIO-1 adapted for electrotransformation of Porphyromonas gingivalis
Tagawa, J., Inoue, T., Naito, M., Sato, K., Kuwahara, T., Nakayama, M., Nakayama, K., Yamashiro, T., & Ohara, N. (2014) · Journal of Microbiological Methods · doi:10.1016/j.mimet.2014.07.032 · Cited by 17 · see citing works
The role of the RgpA–Kgp proteinase–adhesin complexes in the adherence of Porphyromonas gingivalis to fibroblasts
Pathirana, R. D., O’brien-simpson, N. M., Visvanathan, K., Hamilton, J. A., & Reynolds, E. C. (2008) · Microbiology · doi:10.1099/mic.0.2008/019943-0 · Cited by 17 · see citing works
“…by IP: 54.70.40.11 On: Sat, 15 Dec 2018 01:34:35 Previous studies have reported that P. gingivalis fimbriae play a significant role in mediating the adherence of strains ATCC 33277 and 381 to gingival fibroblast cells (Hamada et al., 1994; Kontani et al., 1996; Ogawa et al., 1997).”
BackgroundInfluential
Antagonistic effect of synthetic peptides corresponding to the binding regions within fimbrial subunit protein from Porphyromonas gingivalis to human gingival fibroblasts
Ogawa, T., Ogo, H., & Kinoshita, A. (1997) · Vaccine · doi:10.1016/s0264-410x(96)00127-2 · Cited by 17 · see citing works
Secretion ofPorphyromonas gingivalisFimbrillin Polypeptides by RecombinantStreptococcus gordonii
Sharma, A., Sojar, H. T., Hruby, D. E., Kuramitsu, H. K., & Genco, R. J. (1997) · Biochemical and Biophysical Research Communications · doi:10.1006/bbrc.1997.7306 · Cited by 17 · see citing works
Gingipain enzymes from Porphyromonas gingivalis preferentially bind immobilized extracellular proteins: a mechanism favouring colonization?
McAlister, A. D., Sroka, A., FITZPATRICK, R. E., Quinsey, N. S., Travis, J., Potempa, J., & Pike, R. N. (2008) · Journal of Periodontal Research · doi:10.1111/j.1600-0765.2008.01128.x · Cited by 16 · see citing works
“The Gram negative, anaerobic bacteria Porphyromonas gingivalis is associated with adult periodontal disease in humans and uses a number of adhesin molecules to aid colonisation of the oral cavity, including fimbriae, hemagglutinins and protease/adhesin complexes [1,2,3,4].”
BackgroundMethod
DNA-based adaptive immunity protect host from infection-associated periodontal bone resorption via recognition of Porphyromonas gingivalis virulence component
Han, X., LaRosa, K. B., Kawai, T., & Taubman, M. A. (2013) · Vaccine · doi:10.1016/j.vaccine.2013.09.004 · Cited by 16 · see citing works
“Mutation of the fimA gene, encoding fimbrillin, the major subunit of the fimbriae, prevents Pg to adhere to host cells [7].”
Porphyromonas gingivalis genes isolated by screening for epithelial cell attachment
Duncan, M. J., Emory, S. A., & Almira, E. C. (1996) · Infection and Immunity · doi:10.1128/iai.64.9.3624-3631.1996 · Cited by 16 · see citing works
“The gene encoding fimbrillin (fimA), the protein subunit of fimbriae, was cloned and sequenced (8), and two independently isolated fimA insertion mutants show a loss of ability to adhere not only to saliva-coated hydroxyapatite (25) but also to human epithelial cells (12).”
Background
Induction of immune responses and prevention of alveolar bone loss by intranasal administration of mice with Porphyromonas gingivalis fimbriae and recombinant cholera toxin B subunit
Takahashi, Y., Kumada, H., Hamada, N., Haishima, Y., Ozono, S., Isaka, M., Yasuda, Y., Tochikubo, K., & Umemoto, T. (2007) · Oral Microbiology and Immunology · doi:10.1111/j.1399-302x.2007.00373.x · Cited by 15 · see citing works
Production of monoclonal antibody against FimA protein from Porphyromonas gingivalis in rice cell suspension culture
Kim, B.-G., Kim, S., Kim, N.-S., Huy, N.-X., Choi, Y., Lee, J. Y., Jang, Y., Yang, M., & Kim, T.-G. (2014) · Plant Cell Tissue and Organ Culture (PCTOC) · doi:10.1007/s11240-014-0481-9 · Cited by 13 · see citing works
“Porphyromonas gingivalis, a black-pigmented gramnegative anaerobe, is one of the most important pathogens implicated in the pathogenesis of periodontal disease in adults (Lamont and Jenkinson 1998; Hamada et al. 1994).”
Background
Construction of a sIgA-enhancing anti-Porphyromonas gingivalis FimA vaccine and nasal immunization in mice
Guo, H., Wang, X., Jiang, G., & Yang, P. (2006) · Immunology Letters · doi:10.1016/j.imlet.2006.07.008 · Cited by 13 · see citing works
Purification and characterization of a putative fimbrial protein/receptor of Porphyromonas gingivalis
Dashper, S. G., O’brien-simpson, N. M., Bhogal, P. S., Franzmann, A. D., & Reynolds, E. C. (1998) · Australian Dental Journal · doi:10.1111/j.1834-7819.1998.tb00155.x · Cited by 13 · see citing works
Hyporesponsiveness of inflamed human gingival fibroblasts from patients with chronic periodontal diseases against cell surface components of Porphyromonas gingivalis
Ogawa, T., Ozaki, A., Shimauchi, H., & Uchida, H. (1997) · FEMS Immunology & Medical Microbiology · doi:10.1111/j.1574-695x.1997.tb01023.x · Cited by 12 · see citing works
Peptide Mapping of a Functionally Versatile Fimbrial Adhesin from Porphyromonas gingivalis
Hajishengallis, G. (2007) · International Journal of Peptide Research and Therapeutics · doi:10.1007/s10989-007-9084-1 · Cited by 11 · see citing works
“These binding interactions require the presence of fimbriae since FimA-deficient mutants of P. gingivalis interact poorly with gingival epithelial cells (Hamada et al., 1994; Weinberg et al., 1997).”
Background
Inhibitory effects of azithromycin on the adherence ability of Porphyromonas gingivalis
Kan, P., Sasaki, H., Inaba, K., Watanabe, K., Hamada, N., & Minabe, M. (2019) · Journal of Periodontology · doi:10.1002/jper.18-0559 · Cited by 11 · see citing works
Cloning and characterization of heavy and light chain genes encoding the FimA-specific monoclonal antibodies that inhibit Porphyromonas gingivalis adhesion
Koh, E.-M., Kim, J., Kim, T.-G., Moon, J.-H., Oh, J.-H., Lee, J., & Jang, Y. (2011) · Microbiology and Immunology · doi:10.1111/j.1348-0421.2011.00305.x · Cited by 11 · see citing works
“gingivalis with mutation in the FimA gene fails to attach to human gingival fibroblasts and epithelial cells (7).”
Background
Potential Value of a Rice Protein Extract, Containing Proteinaceous Inhibitors against Cysteine Proteinases fromPorphyromonas gingivalis, for Managing Periodontal Diseases
Taiyoji, M., Yamanaka, T., Tsuno, T., & Ohtsubo, S. (2013) · Bioscience Biotechnology and Biochemistry · doi:10.1271/bbb.120585 · Cited by 11 · see citing works
Production of Monoclonal Antibodies Specific to FimA of Porphyromonas gingivalis and Their Inhibitory Activity on Bacterial Binding
Koh, E.-M., Kim, J., Lee, J.-Y., & Kim, T.-G. (2009) · Immune Network · doi:10.4110/in.2009.9.5.203 · Cited by 11 · see citing works
Identification of a new genetic variant (G231N, E232T, N235D) of peptidylarginine deiminase from P. gingivalis in advanced periodontitis
Bereta, G. P., Strzelec, K., Łazarz‐Bartyzel, K., Dziedzic, A., Nowakowska, Z., Krutyhołowa, A., Bielecka, E., Kantyka, T., Grabiec, A. M., Kaczmarzyk, T., et al. (2024) · Frontiers in Immunology · doi:10.3389/fimmu.2024.1355357 · Cited by 10 · see citing works
Salivary immunoglobulin A production against a synthetic oligopeptide antigen of Actinobacillus actinomycetemcomitans fimbriae
Honma, K., Kato, T., & Okuda, K. (1999) · Oral Microbiology and Immunology · doi:10.1034/j.1399-302x.1999.140504.x · Cited by 10 · see citing works
The gingipains from Porphyromonas gingivalis do not directly induce osteoclast differentiation in primary mouse bone marrow cultures
FITZPATRICK, R. E., Campbell, P. D., Sivagurunathan, S., Pagel, C. N., Potempa, J., Mackie, E. J., & Pike, R. N. (2008) · Journal of Periodontal Research · doi:10.1111/j.1600-0765.2008.01151.x · Cited by 8 · see citing works
Reduced fimbria-associated activities of Porphyromonas gingivalis induced by recombinant fimbrial expression
Takahashi, Y., Yoshimoto, H., Kato, D., Hamada, N., Arai, M., & Umemoto, T. (2001) · FEMS Microbiology Letters · doi:10.1111/j.1574-6968.2001.tb10524.x · Cited by 7 · see citing works
Mesaconate from Bacillus subtilis R0179 Supernatant Attenuates Periodontitis by Inhibiting Porphyromonas gingivalis in Mice
Zhao, W., Ji, L., Li, J., Liu, D., Yan, C., Zhang, C., Wang, X., Liu, Y., & Zheng, S. (2024) · Journal of Periodontal Research · doi:10.1111/jre.13363 · Cited by 6 · see citing works

Involvement of minor components associated with the FimA fimbriae of Porphyromonas gingivalis in adhesive functions
Nishiyama, S.-I., MURAKAMI, Y., Nagata, H., Shizukuishi, S., Kawagishi, I., & Yoshimura, F. (2007) · Microbiology · doi:10.1099/mic.0.2006/005561-0 · Cited by 83 · see citing works
Transformation and Expression of a Cloned fimA Gene in Porphyromonas gingivalis
Takahashi, Y., Kato, D., Hamada, N., Yoshimoto, H., & Umemoto, T. (1999) · Infection and Immunity · doi:10.1128/iai.67.4.2013-2018.1999 · Cited by 7 · see citing works
FimB Regulates FimA Fimbriation in Porphyromonas gingivalis
Nagano, K., Hasegawa, Y., Murakami, Y., Nishiyama, S., & Yoshimura, F. (2010) · Journal of Dental Research · doi:10.1177/0022034510370089 · Cited by 47 · see citing works
Porphyromonas gingivalis FimA fimbriae: Roles of the fim gene cluster in the fimbrial assembly and antigenic heterogeneity among fimA genotypes
Nagano, K., Abiko, Y., Yoshida, Y., & Yoshimura, F. (2012) · Journal of Oral Biosciences · doi:10.1016/j.job.2012.07.002 · Cited by 13 · see citing works
Inactivation of the Porphyromonas gingivalis fimA gene blocks periodontal damage in gnotobiotic rats
Malek, R. L., Fisher, J. G., Caleca, A., Stinson, M. W., van Oss, C. J., Lee, J. Y., Cho, M. I., Genco, R. J., Evans, R. T., & Dyer, D. W. (1994) · Journal of Bacteriology · doi:10.1128/jb.176.4.1052-1059.1994 · Cited by 186 · see citing works

Authors: Hamada, N., Watanabe, K., Sasakawa, C., Yoshikawa, M., Yoshimura, F., & Umemoto, T.
Affiliations: Kanagawa Dental University (JP)
ORCID: Watanabe, K
Yoshimura, F
Venue: Infection and Immunity
Publisher: American Society for Microbiology
Published: 1994-05
Volume: 62
Issue: 5
Pages: 1696-1704
ISSN: 0019-9567 (ISSN-L)
1098-5522
DOI: 10.1128/iai.62.5.1696-1704.1994
PMC: PMC186386
OpenAlex: W2111125148
Cited by: 124
References: 45
Citations by year: 2026: 3 · 2025: 1 · 2024: 2 · 2023: 1 · 2022: 1 · 2019: 5 · 2018: 2 · 2017: 1 · 2016: 2 · 2015: 4 · 2014: 4 · 2013: 3 · 2012: 2
FWCI: 13.33
Type: journal article
Language: en
Open access: green
europepmc.org
Licence: journals.asm.org/non-commercial-tdm-license (tdm)
Locations: Infection and Immunity (publishedVersion)
Europe PMC (PubMed Central) (publishedVersion)
PubMed Central (publishedVersion)
PubMed (publishedVersion)
Topics: Health Sciences › Dentistry › Periodontics › Oral microbiology and periodontitis research
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Biochemical and Structural Characterization
Life Sciences › Immunology and Microbiology › Microbiology › Microbial infections and disease research
Keywords: Porphyromonas gingivalis, fimA gene, fimbriae, bacterial adhesion, gingival fibroblasts, gingival epithelial cells, homologous recombination, gene disruption, erythromycin resistance, hemagglutination, surface hydrophobicity, periodontal pathogens, host-bacteria interactions
MeSH terms: Bacterial Adhesion, Bacterial Proteins, Fibroblasts, Gingiva, Hemagglutination, Humans, Mutation, Fimbriae, Bacterial, Porphyromonas gingivalis, Fimbriae Proteins

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review

Construction and characterization of a fimA mutant of Porphyromonas gingivalis

Abstract

Integrity signals

Claims the paper makes

Claims other papers make about this one

Cites

Cited by

Related

Record

About these checks