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Positive selection procedure for entrapment of insertion sequence elements in gram-negative bacteria
Open accessAbstract
We constructed the broad-host-range plasmid pUCD800 containing the sacB gene of Bacillus subtilis for use in the positive selection and isolation of insertion sequence (IS) elements in gram-negative bacteria. Cells containing pUCD800 do not grow on medium containing 5% sucrose unless the sacB gene is inactivated. By using pUCD800, we isolated a 1.4-kilobase putative IS element from Agrobacterium tumefaciens NT1RE by selection for growth on sucrose medium. This putative IS element appears to be unique to Agrobacterium strains.
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Cited by
Link, A. J., Phillips, D., & Church, G. M. (1997) · Journal of Bacteriology · doi:10.1128/jb.179.20.6228-6237.1997 · Cited by 874
Ried, J. L., & Collmer, A. (1987) · Gene · doi:10.1016/0378-1119(87)90127-2 · Cited by 351
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Chemla, Y., Sweeney, C. J., Wozniak, C. A., & Voigt, C. A. (2025) · Nature Microbiology · doi:10.1038/s41564-024-01918-0 · Cited by 142
The Molecular Toolset and Techniques Required to Build Cyanobacterial Cell Factories
Opel, F., Axmann, I. M., & Klähn, S. (2022) · Advances in Biochemical Engineering/Biotechnology · doi:10.1007/10_2022_210 · Cited by 7
Metadata
- Authors
- Gay, P., Le Coq, D., Steinmetz, M., Berkelman, T., & Kado, C. I.
- Venue
- Journal of Bacteriology
- Publisher
- American Society for Microbiology
- Published
- 1985-11
- Volume
- 164
- Issue
- 2
- Pages
- 918-921
- ISSN
- 0021-9193 (ISSN-L)
- 1098-5530
- OpenAlex
- W1832001947
- Cited by
- 487
- References
- 0
- FWCI
- 1.94
- Type
- journal article
- Language
- en
- Open access
- bronze
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- CoChat. (2026). Citation check: Positive selection procedure for entrapment of insertion sequence elements in gram-negative bacteria. https://app.cochat.ai/papers/10.1128/jb.164.2.918-921.1985
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- Gay, P., Le Coq, D., Steinmetz, M., Berkelman, T., & Kado, C. I. (1985). Positive selection procedure for entrapment of insertion sequence elements in gram-negative bacteria. Journal of Bacteriology. https://doi.org/10.1128/jb.164.2.918-921.1985
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