The Molecular Toolset and Techniques Required to Build Cyanobacterial Cell Factories

Enzymatic assembly of DNA molecules up to several hundred kilobases

Gibson, D. G., Young, L., Chuang, R.-Y., Venter, J. C., Hutchison, C. A., & Smith, H. O. (2009) · Nature Methods · doi:10.1038/nmeth.1318 · Cited by 9,742 · see citing works

Generic Assignments, Strain Histories and Properties of Pure Cultures of Cyanobacteria

Rippka, R., Stanier, R. Y., Deruelles, J., Herdman, M., & Waterbury, J. B. (1979) · Microbiology · doi:10.1099/00221287-111-1-1 · Cited by 7,606 · see citing works

Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression

Qi, L. S., Larson, M. H., Gilbert, L. A., Doudna, J. A., Weissman, J. S., Arkin, A. P., & Lim, W. A. (2013) · Cell · doi:10.1016/j.cell.2013.02.022 · Cited by 5,016 · see citing works

An updated evolutionary classification of CRISPR–Cas systems

Makarova, K. S., Wolf, Y. I., Alkhnbashi, O. S., Costa, F., Shah, S. A., Saunders, S. J., Barrangou, R., Brouns, S. J. J., Charpentier, E., Haft, D. H., et al. (2015) · Nature Reviews Microbiology · doi:10.1038/nrmicro3569 · Cited by 2,504 · see citing works

A One Pot, One Step, Precision Cloning Method with High Throughput Capability

Engler, C., Kandzia, R., & Marillonnet, S. (2008) · PLoS ONE · doi:10.1371/journal.pone.0003647 · Cited by 2,398 · see citing works

Automated design of synthetic ribosome binding sites to control protein expression

Salis, H. M., Mirsky, E. A., & Voigt, C. A. (2009) · Nature Biotechnology · doi:10.1038/nbt.1568 · Cited by 1,844 · see citing works

Regulatory RNAs in Bacteria

Waters, L. S., & Storz, G. (2009) · Cell · doi:10.1016/j.cell.2009.01.043 · Cited by 1,599 · see citing works

A Modular Cloning System for Standardized Assembly of Multigene Constructs

Weber, E., Engler, C., Gruetzner, R., Werner, S., & Marillonnet, S. (2011) · PLoS ONE · doi:10.1371/journal.pone.0016765 · Cited by 1,385 · see citing works

New Unstable Variants of Green Fluorescent Protein for Studies of Transient Gene Expression in Bacteria

Andersen, J. B., Sternberg, C., Poulsen, L. K., Bjørn, S. P., Givskov, M., & Molin, S. (1998) · Applied and Environmental Microbiology · doi:10.1128/aem.64.6.2240-2246.1998 · Cited by 1,036 · see citing works

Golden Gate Shuffling: A One-Pot DNA Shuffling Method Based on Type IIs Restriction Enzymes

Engler, C., Gruetzner, R., Kandzia, R., & Marillonnet, S. (2009) · PLoS ONE · doi:10.1371/journal.pone.0005553 · Cited by 1,028 · see citing works

Gene regulation by riboswitches

Mandal, M., & Breaker, R. R. (2004) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm1403 · Cited by 913 · see citing works

Metabolic engineering of Escherichia coli using synthetic small regulatory RNAs

Na, D., Yoo, S. M., Chung, H., Park, H., Park, J. H., & Lee, S. Y. (2013) · Nature Biotechnology · doi:10.1038/nbt.2461 · Cited by 623 · see citing works

Engineering a platform for photosynthetic isoprene production in cyanobacteria, using Synechocystis as the model organism

Lindberg, P., Park, S., & Melis, A. (2010) · Metabolic Engineering · doi:10.1016/j.ymben.2009.10.001 · Cited by 596 · see citing works

[83] Conjugal transfer of DNA to cyanobacteria

Elhai, J., & Wölk, C. P. (1988) · Methods in enzymology on CD-ROM/Methods in enzymology · doi:10.1016/0076-6879(88)67086-8 · Cited by 568 · see citing works

Engineered riboregulators enable post-transcriptional control of gene expression

Isaacs, F. J., Dwyer, D. J., Ding, C., Pervouchine, D. D., Cantor, C. R., & Collins, J. J. (2004) · Nature Biotechnology · doi:10.1038/nbt986 · Cited by 547 · see citing works

Fatty acid production in genetically modified cyanobacteria

Liu, X., Sheng, J., & Curtiss III, R. (2011) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1103014108 · Cited by 532 · see citing works

The molecular biology of cyanobacteria

(1995) · Photosynthesis Research · doi:10.1007/bf00032589 · Cited by 510 · see citing works

Engineering cyanobacteria to generate high-value products

Ducat, D. C., Way, J. C., & Silver, P. A. (2011) · Trends in biotechnology · doi:10.1016/j.tibtech.2010.12.003 · Cited by 507 · see citing works

Use of a conditionally lethal gene in Anabaena sp. strain PCC 7120 to select for double recombinants and to entrap insertion sequences

Cai, Y., & Wölk, C. P. (1990) · Journal of Bacteriology · doi:10.1128/jb.172.6.3138-3145.1990 · Cited by 500 · see citing works

Positive selection procedure for entrapment of insertion sequence elements in gram-negative bacteria

Gay, P., Coq, D. L., Steinmetz, M. O., et al. (1985) · Journal of Bacteriology · doi:10.1128/jb.164.2.918-921.1985 · Cited by 496 · see citing works

Origins and evolution of CRISPR-Cas systems

Koonin, E. V., & Makarova, K. S. (2019) · Philosophical Transactions of the Royal Society B: Biological Sciences · doi:10.1098/rstb.2018.0087 · Cited by 494 · see citing works

Potential of industrial biotechnology with cyanobacteria and eukaryotic microalgae

Wijffels, R. H., Kruse, O., & Hellingwerf, K. J. (2013) · Current Opinion in Biotechnology · doi:10.1016/j.copbio.2013.04.004 · Cited by 404 · see citing works

Idempotent Vector Design for Standard Assembly of Biobricks

Knight, T. (2003) · doi:10.21236/ada457791 · Cited by 397 · see citing works

Cyanobacterial conversion of carbon dioxide to 2,3-butanediol

Oliver, J. W. K., Machado, I. M. P., Yoneda, H., & Atsumi, S. (2013) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1213024110 · Cited by 382 · see citing works

Riboswitches: Structures and MechanismsPreprint

Garst, A. D., Edwards, A. L., & Batey, R. (2010) · Cold Spring Harbor Perspectives in Biology · doi:10.1101/cshperspect.a003533 · Cited by 373 · see citing works

Synechococcus elongatus UTEX 2973, a fast growing cyanobacterial chassis for biosynthesis using light and CO2

Yu, J., Liberton, M., Cliften, P. F., Head, R. D., Jacobs, J., Smith, R. D., Koppenaal, D., Brand, J. J., & Pakrasi, H. B. (2015) · Scientific Reports · doi:10.1038/srep08132 · Cited by 372 · see citing works

An experimentally anchored map of transcriptional start sites in the model cyanobacterium Synechocystis sp. PCC6803

Mitschke, J., Georg, J., Scholz, I., Sharma, C. M., Dienst, D. D., Bantscheff, J., Voß, B., Steglich, C., Wilde, A., Vogel, J., et al. (2011) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1015154108 · Cited by 361 · see citing works

Small Proteins Can No Longer Be Ignored

Storz, G., Wolf, Y. I., & Ramamurthi, K. S. (2014) · Annual Review of Biochemistry · doi:10.1146/annurev-biochem-070611-102400 · Cited by 359 · see citing works

Design and characterization of molecular tools for a Synthetic Biology approach towards developing cyanobacterial biotechnology

Huang, H.-H., Camsund, D., Lindblad, P. C., & Heidorn, T. (2010) · Nucleic Acids Research · doi:10.1093/nar/gkq164 · Cited by 351 · see citing works

Natural genetic transformation: prevalence, mechanisms and function

Johnsborg, O., Eldholm, V., & Håvarstein, L. S. (2007) · Research in Microbiology · doi:10.1016/j.resmic.2007.09.004 · Cited by 338 · see citing works

Reduction of conjugal transfer efficiency by three restriction activities of Anabaena sp. strain PCC 7120

Elhai, J., Vepritskiy, A., Muro-Pastor, A. M., Flores, E., & Wolk, C. P. (1997) · Journal of Bacteriology · doi:10.1128/jb.179.6.1998-2005.1997 · Cited by 334 · see citing works

Regulatory mechanisms employed by cis-encoded antisense RNAs

Brantl, S. (2007) · Current Opinion in Microbiology · doi:10.1016/j.mib.2007.03.012 · Cited by 332 · see citing works

Construction of shuttle vectors capable of conjugative transfer from Escherichia coli to nitrogen-fixing filamentous cyanobacteria

Wolk, C. P., Vonshak, A., Kehoe, P., & Elhai, J. (1984) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.81.5.1561 · Cited by 328 · see citing works

Toxins produced in cyanobacterial water blooms - toxicity and risks

Bláha, L., Babica, P., & Maršálek, B. (2009) · Interdisciplinary Toxicology · doi:10.2478/v10102-009-0006-2 · Cited by 316 · see citing works

Adaptive laboratory evolution — harnessing the power of biology for metabolic engineering

Portnoy, V. A., Bezdan, D., & Zengler, K. (2011) · Current Opinion in Biotechnology · doi:10.1016/j.copbio.2011.03.007 · Cited by 309 · see citing works

Correlations between Shine-Dalgarno Sequences and Gene Features Such as Predicted Expression Levels and Operon Structures

Ma, J., Campbell, A., & Karlin, S. (2002) · Journal of Bacteriology · doi:10.1128/jb.184.20.5733-5745.2002 · Cited by 308 · see citing works

Cpf1 Is a Versatile Tool for CRISPR Genome Editing Across Diverse Species of Cyanobacteria

Ungerer, J., & Pakrasi, H. B. (2016) · Scientific Reports · doi:10.1038/srep39681 · Cited by 308 · see citing works

Influence of light on accumulation of photosynthesis-specific transcripts in the cyanobacterium Synechocystis 6803

Mohamed, A. J., & Jansson, C. (1989) · Plant Molecular Biology · doi:10.1007/bf00016024 · Cited by 305 · see citing works

Synthetic biology of cyanobacteria: unique challenges and opportunities

Berla, B. M., Saha, R., Immethun, C. M., Maranas, C. D., Moon, T. S., & Pakrasi, H. B. (2013) · Frontiers in Microbiology · doi:10.3389/fmicb.2013.00246 · Cited by 305 · see citing works

Predictive design of mRNA translation initiation region to control prokaryotic translation efficiency

Seo, S. W., Yang, J.-S., Kim, I., Yang, J., Min, B. E., Kim, S., & Jung, G. Y. (2013) · Metabolic Engineering · doi:10.1016/j.ymben.2012.10.006 · Cited by 300 · see citing works

Transformation in the cyanobacterium Synechocystis sp. 6803

Grigorieva, G., & Shestakov, S. V. (1982) · FEMS Microbiology Letters · doi:10.1111/j.1574-6968.1982.tb08289.x · Cited by 288 · see citing works

Transcription from efficient promoters can interfere with plasmid replication and diminish expression of plasmid specified genes.

Stueber, D., & Bujard, H. (1982) · The EMBO Journal · doi:10.1002/j.1460-2075.1982.tb01329.x · Cited by 278 · see citing works

Adaptive laboratory evolution principles and applications in industrial biotechnology

Mavrommati, M., Daskalaki, A., Papanikolaou, S., & Aggelis, G. (2021) · Biotechnology Advances · doi:10.1016/j.biotechadv.2021.107795 · Cited by 269 · see citing works

Ploidy in cyanobacteria

Griese, M., Lange, C., & Soppa, J. (2011) · FEMS Microbiology Letters · doi:10.1111/j.1574-6968.2011.02368.x · Cited by 264 · see citing works

Genetic parts to program bacteria

Voigt, C. A. (2006) · Current Opinion in Biotechnology · doi:10.1016/j.copbio.2006.09.001 · Cited by 252 · see citing works

Multiple Gene Repression in Cyanobacteria Using CRISPRi

Yao, L., Cengic, I., Anfelt, J., & Hudson, E. P. (2016) · ACS Synthetic Biology · doi:10.1021/acssynbio.5b00264 · Cited by 246 · see citing works

Engineering Cyanobacteria To Synthesize and Export Hydrophilic Products

Niederholtmeyer, H., Wolfstädter, B. T., Savage, D. F., Silver, P. A., & Way, J. C. (2010) · Applied and Environmental Microbiology · doi:10.1128/aem.00202-10 · Cited by 243 · see citing works

Synthetic Biology in Cyanobacteria

Heidorn, T., Camsund, D., Huang, H.-H., Lindberg, P., Oliveira, P., Stensjö, K., & Lindblad, P. C. (2011) · Methods in enzymology on CD-ROM/Methods in enzymology · doi:10.1016/b978-0-12-385075-1.00024-x · Cited by 235 · see citing works

Molecular Nature of Two Nonconjugative Plasmids Carrying Drug Resistance Genes

Guerry, P., van Embden, J., & Falkow, S. (1974) · Journal of Bacteriology · doi:10.1128/jb.117.2.619-630.1974 · Cited by 233 · see citing works

CRISPR/Cas9 mediated targeted mutagenesis of the fast growing cyanobacterium Synechococcus elongatus UTEX 2973

Wendt, K. E., Ungerer, J., Cobb, R. E., Zhao, H., & Pakrasi, H. B. (2016) · Microbial Cell Factories · doi:10.1186/s12934-016-0514-7 · Cited by 232 · see citing works

CRISPR-Cas9 for the genome engineering of cyanobacteria and succinate production

Li, H., Shen, C. R., Huang, C.-H., Sung, L., Wu, M., & Hu, Y. (2016) · Metabolic Engineering · doi:10.1016/j.ymben.2016.09.006 · Cited by 232 · see citing works

Genetic tools for cyanobacteria

Koksharova, O. A. (2002) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-001-0864-9 · Cited by 228 · see citing works

New applications of synthetic biology tools for cyanobacterial metabolic engineering

Santos-Merino, M., Singh, A. K., & Ducat, D. C. (2019) · Frontiers in Bioengineering and Biotechnology · doi:10.3389/fbioe.2019.00033 · Cited by 222 · see citing works

Expression of the Anabaena hetR gene from a copper-regulated promoter leads to heterocyst differentiation under repressing conditions

Buikema, W. J., & Haselkorn, R. (2001) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.051624898 · Cited by 221 · see citing works

Global metabolic rewiring for improved CO2 fixation and chemical production in cyanobacteria

Kanno, M., Carroll, A. L., & Atsumi, S. (2017) · Nature Communications · doi:10.1038/ncomms14724 · Cited by 220 · see citing works

Transformation in <i>Agmenellum quadruplicatum</i>

Stevens, S. E., & Porter, R. D. (1980) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.77.10.6052 · Cited by 212 · see citing works

Synthetic Biology Toolbox for Controlling Gene Expression in the Cyanobacterium Synechococcus sp. strain PCC 7002

Markley, A. L., Begemann, M. B., Clarke, R., Gordon, G. C., & Pfleger, B. F. (2014) · ACS Synthetic Biology · doi:10.1021/sb500260k · Cited by 212 · see citing works

Application of synthetic biology in cyanobacteria and algae

Wang, B., Wang, J., Zhang, W., & Meldrum, D. R. (2012) · Frontiers in Microbiology · doi:10.3389/fmicb.2012.00344 · Cited by 211 · see citing works

Evidence for genetic transformation in blue-green alga Anacystis nidulans

Shestakov, S. V., & Khyen, N. T. (1970) · Molecular and General Genetics MGG · doi:10.1007/bf00441199 · Cited by 208 · see citing works

Photosynthesis driven conversion of carbon dioxide to fatty alcohols and hydrocarbons in cyanobacteria

Tan, X., Yao, L., Gao, Q., Wang, W., Qi, F., & Lü, X. (2011) · Metabolic Engineering · doi:10.1016/j.ymben.2011.01.001 · Cited by 202 · see citing works

Synthetic Riboswitches That Induce Gene Expression in Diverse Bacterial Species

Topp, S., Reynoso, C. M. K., Seeliger, J. C., Goldlust, I. S., Desai, S. K., Murat, D., Shen, A., Puri, A. W., Komeili, A., Bertozzi, C. R., et al. (2010) · Applied and Environmental Microbiology · doi:10.1128/aem.01537-10 · Cited by 197 · see citing works

AQUA Cloning: A Versatile and Simple Enzyme-Free Cloning Approach

Beyer, H. M., Gonschorek, P., Samodelov, S. L., Meier, M., Weber, W., & Zurbriggen, M. D. (2015) · PLoS ONE · doi:10.1371/journal.pone.0137652 · Cited by 193 · see citing works

Newly discovered Synechococcus sp. PCC 11901 is a robust cyanobacterial strain for high biomass production

Włodarczyk, A., Selão, T. T., Norling, B., & Nixon, P. J. (2020) · Communications Biology · doi:10.1038/s42003-020-0910-8 · Cited by 192 · see citing works

CRISPR interference as a titratable, trans-acting regulatory tool for metabolic engineering in the cyanobacterium Synechococcus sp. strain PCC 7002

Gordon, G. C., Korosh, T. C., Cameron, J. C., Markley, A. L., Begemann, M. B., & Pfleger, B. F. (2016) · Metabolic Engineering · doi:10.1016/j.ymben.2016.07.007 · Cited by 189 · see citing works

Cyanogate: a modular cloning suite for engineering cyanobacteria based on the plant moclo syntax

Vasudevan, R., Gale, G. A. R., Schiavon, A. A., Puzorjov, A., Malin, J., Gillespie, M. D., Vavitsas, K., Zulkower, V., Wang, B., Howe, C. J., et al. (2019) · Plant Physiology · doi:10.1104/pp.18.01401 · Cited by 189 · see citing works

Broad-host-range vector system for synthetic biology and biotechnology in cyanobacteria

Taton, A., Unglaub, F., Wright, N. E., Zeng, W. Y., Paz-Yepes, J., Brahamsha, B., Palenik, B., Peterson, T. C., Haerizadeh, F., Golden, S. S., et al. (2014) · Nucleic Acids Research · doi:10.1093/nar/gku673 · Cited by 186 · see citing works

Mutational Analysis of Genes Involved in Pilus Structure, Motilityand Transformation Competency in the Unicellular Motile CyanobacteriumSynechocystis sp. PCC6803

Yoshihara, S., Geng, X., Okamoto, S., Yura, K., Murata, T., Gō, M., Ohmori, M., & Ikeuchi, M. (2001) · Plant and Cell Physiology · doi:10.1093/pcp/pce007 · Cited by 186 · see citing works

Ethylene Synthesis and Regulated Expression of Recombinant Protein in Synechocystis sp. PCC 6803

Guerrero, F., Carbonell, V., Cossu, M., Correddu, D., & Jones, P. R. (2012) · PLoS ONE · doi:10.1371/journal.pone.0050470 · Cited by 183 · see citing works

Wide-dynamic-range promoters engineered for cyanobacteria

Huang, H.-H., & Lindblad, P. C. (2013) · Journal of Biological Engineering · doi:10.1186/1754-1611-7-10 · Cited by 172 · see citing works

Genetic evidence of a major role for glucose-6-phosphate dehydrogenase in nitrogen fixation and dark growth of the cyanobacterium Nostoc sp. strain ATCC 29133

Summers, M. L., Wallis, J. G., Campbell, E. L., & Meeks, J. C. (1995) · Journal of Bacteriology · doi:10.1128/jb.177.21.6184-6194.1995 · Cited by 172 · see citing works

Engineering cyanobacteria for photosynthetic production of 3-hydroxybutyrate directly from CO2

Wang, B., Pugh, S., Nielsen, D. R., Zhang, W., & Meldrum, D. R. (2013) · Metabolic Engineering · doi:10.1016/j.ymben.2013.01.001 · Cited by 170 · see citing works

Discovery of a super-strong promoter enables efficient production of heterologous proteins in cyanobacteria

Zhou, J., Zhang, H., Meng, H., Zhu, Y., Bao, G., Zhang, Y., Li, Y., & Ma, Y. (2014) · Scientific Reports · doi:10.1038/srep04500 · Cited by 169 · see citing works

Replication and conjugative mobilization of broad host-range IncQ plasmids

Meyer, R. J. (2009) · Plasmid · doi:10.1016/j.plasmid.2009.05.001 · Cited by 167 · see citing works

Modular engineering for efficient photosynthetic biosynthesis of 1-butanol from CO ₂ in cyanobacteria

Liu, X., Miao, R., Lindberg, P., & Lindblad, P. (2019) · Energy &amp; Environmental Science · doi:10.1039/c9ee01214a · Cited by 167 · see citing works

Evaluation of promoters and ribosome binding sites for biotechnological applications in the unicellular cyanobacterium Synechocystis sp. PCC 6803

Englund, E., Liang, F., & Lindberg, P. (2016) · Scientific Reports · doi:10.1038/srep36640 · Cited by 166 · see citing works

CRISPR interference (CRISPRi) for gene regulation and succinate production in cyanobacterium S. elongatus PCC 7942

Huang, C.-H., Shen, C. R., Li, H., Sung, L.-Y., Wu, M.-Y., & Hu, Y.-C. (2016) · Microbial Cell Factories · doi:10.1186/s12934-016-0595-3 · Cited by 164 · see citing works

The role of HetN in maintenance of the heterocyst pattern in Anabaena sp. PCC 7120

Callahan, S. M., & Buikema, W. J. (2001) · Molecular Microbiology · doi:10.1046/j.1365-2958.2001.02437.x · Cited by 162 · see citing works

RBSDesigner : software for designing synthetic ribosome binding sites that yields a desired level of protein expression

Na, D., & Lee, D. (2010) · Bioinformatics · doi:10.1093/bioinformatics/btq458 · Cited by 162 · see citing works

Pooled CRISPRi screening of the cyanobacterium Synechocystis sp PCC 6803 for enhanced industrial phenotypes

Yao, L., Shabestary, K., Björk, S., Asplund‐Samuelsson, J., Joensson, H. N., Jahn, M., & Hudson, E. P. (2020) · Nature Communications · doi:10.1038/s41467-020-15491-7 · Cited by 158 · see citing works

Maximizing PHB content in Synechocystis sp. PCC 6803: a new metabolic engineering strategy based on the regulator PirC

Koch, M., Bruckmoser, J., Scholl, J., Hauf, W., Rieger, B., & Forchhammer, K. (2020) · Microbial Cell Factories · doi:10.1186/s12934-020-01491-1 · Cited by 156 · see citing works

Specialized Techniques for Site-Directed Mutagenesis in Cyanobacteria

Clérico, E. M., Ditty, J. L., & Golden, S. S. (2007) · Methods in molecular biology · doi:10.1007/978-1-59745-257-1_11 · Cited by 154 · see citing works

Engineering of photosynthetic mannitol biosynthesis from CO2 in a cyanobacterium

Jacobsen, J. H., & Frigaard, N. (2013) · Metabolic Engineering · doi:10.1016/j.ymben.2013.11.004 · Cited by 153 · see citing works

Metabolic Engineering of Synechocystis sp. PCC 6803 for Production of the Plant Diterpenoid Manoyl Oxide

Englund, E., Andersen‐Ranberg, J., Miao, R., Hamberger, B. R., & Lindberg, P. (2015) · ACS Synthetic Biology · doi:10.1021/acssynbio.5b00070 · Cited by 152 · see citing works

Theophylline-Dependent Riboswitch as a Novel Genetic Tool for Strict Regulation of Protein Expression in Cyanobacterium Synechococcus elongatus PCC 7942

Nakahira, Y., Ogawa, A., Asano, H., Oyama, T., & Tozawa, Y. (2013) · Plant and Cell Physiology · doi:10.1093/pcp/pct115 · Cited by 143 · see citing works

Application of bioluminescence to the study of circadian rhythms in cyanobacteria

Andersson, C. R., Tsinoremas, N. F., Shelton, J. L., Lebedeva, N. V., Yarrow, J., Min, H., & Golden, S. S. (2000) · Methods in enzymology on CD-ROM/Methods in enzymology · doi:10.1016/s0076-6879(00)05511-7 · Cited by 142 · see citing works

Enhancing power density of biophotovoltaics by decoupling storage and power delivery

Saar, K. L., Bombelli, P., Lea‐Smith, D. J., Call, T. P., Aro, E., Müller, T., Howe, C. J., & Knowles, T. P. J. (2018) · Nature Energy · doi:10.1038/s41560-017-0073-0 · Cited by 140 · see citing works

Isolation and complementation of mutants of Anabaena sp. strain PCC 7120 unable to grow aerobically on dinitrogen

Wölk, C. P., Cai, Y., Cardemil, L., Flores, E., Höhn, B., Murry, M. A., Schmetterer, G., Schrautemeier, B., & Wilson, R. (1988) · Journal of Bacteriology · doi:10.1128/jb.170.3.1239-1244.1988 · Cited by 139 · see citing works

CRISPR-Based Technologies for Metabolic Engineering in Cyanobacteria

Behler, J., Vijay, D., Hess, W. R., & Akhtar, M. K. (2018) · Trends in biotechnology · doi:10.1016/j.tibtech.2018.05.011 · Cited by 138 · see citing works

A Critical Review of Genome Editing and Synthetic Biology Applications in Metabolic Engineering of Microalgae and Cyanobacteria

Ng, I., Keskin, B. B., & Tan, S. (2020) · Biotechnology Journal · doi:10.1002/biot.201900228 · Cited by 135 · see citing works

Construction and Analysis of a Recombinant Cyanobacterium Expressing a Chromosomally Inserted Gene for an Ethylene-Forming Enzyme at the psbAI Locus

Takahama, K., Matsuoka, M., Nagahama, K., & Ogawa, T. (2003) · Journal of Bioscience and Bioengineering · doi:10.1016/s1389-1723(03)80034-8 · Cited by 132 · see citing works

Molecular Characterization of the R Factors Implicated in the Carbenicillin Resistance of a Sequence of Pseudomonas aeruginosa Strains Isolated from Burns

Ingram, L. C., Richmond, M. H., & SYKES, R. B. (1973) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.3.2.279 · Cited by 131 · see citing works

Rewiring hydrogenase-dependent redox circuits in cyanobacteria

Ducat, D. C., Sachdeva, G., & Silver, P. A. (2011) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1016026108 · Cited by 130 · see citing works

A two‐component signal transduction system involved in nickel sensing in the cyanobacterium Synechocystis sp. PCC 6803

López‐Maury, L., García‐Domínguez, M., Florencio, F. J., & Reyes, J. C. (2002) · Molecular Microbiology · doi:10.1046/j.1365-2958.2002.02741.x · Cited by 129 · see citing works

Comparative genomics reveals the molecular determinants of rapid growth of the cyanobacterium Synechococcus elongatus UTEX 2973

Ungerer, J. L., Wendt, K. E., Hendry, J. I., Maranas, C. D., & Pakrasi, H. B. (2018) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1814912115 · Cited by 128 · see citing works

Regulation of Gene Expression in Diverse Cyanobacterial Species by Using Theophylline-Responsive Riboswitches

Ma, A. T., Schmidt, C. M., & Golden, J. W. (2014) · Applied and Environmental Microbiology · doi:10.1128/aem.01697-14 · Cited by 126 · see citing works

High-Throughput Functional Analysis of the Synechococcus elongatus PCC 7942 Genome

Holtman, C. K. (2005) · DNA Research · doi:10.1093/dnares/12.2.103 · Cited by 121 · see citing works

Genome Features and Biochemical Characteristics of a Robust, Fast Growing and Naturally Transformable Cyanobacterium Synechococcus elongatus PCC 11801 Isolated from India

Jaiswal, D., Sengupta, A., Sohoni, S. V., Sengupta, S., Phadnavis, A. G., Pakrasi, H. B., & Wangikar, P. P. (2018) · Scientific Reports · doi:10.1038/s41598-018-34872-z · Cited by 120 · see citing works

Isolation and complementation of nitrogen fixation mutants of the cyanobacterium Anabaena sp. strain PCC 7120

Buikema, W. J., & Haselkorn, R. (1991) · Journal of Bacteriology · doi:10.1128/jb.173.6.1879-1885.1991 · Cited by 119 · see citing works

Expanding the Potential of CRISPR-Cpf1-Based Genome Editing Technology in the Cyanobacterium Anabaena PCC 7120

Niu, T., Lin, G., Xie, L.-R., Wang, Z.-Q., Xing, W., Zhang, J., & Zhang, C. (2018) · ACS Synthetic Biology · doi:10.1021/acssynbio.8b00437 · Cited by 119 · see citing works

Combinatorial optimization of cyanobacterial 2,3-butanediol production

Oliver, J. W. K., Machado, I. M. P., Yoneda, H., & Atsumi, S. (2014) · Metabolic Engineering · doi:10.1016/j.ymben.2014.01.001 · Cited by 116 · see citing works

Toolboxes for cyanobacteria: recent advances and future direction

Sun, T., Li, S., Song, X., Diao, J., Chen, L., & Zhang, W. (2018) · Biotechnology Advances · doi:10.1016/j.biotechadv.2018.04.007 · Cited by 116 · see citing works

Construction of promoter probe vectors for Synechocystis sp. PCC 6803 using the light-emitting reporter systems Gfp and LuxAB

Kunert, A., Hagemann, M., & Erdmann, N. (2000) · Journal of Microbiological Methods · doi:10.1016/s0167-7012(00)00162-7 · Cited by 113 · see citing works

Development and optimization of genetic toolboxes for a fast-growing cyanobacterium Synechococcus elongatus UTEX 2973

Li, S., Sun, T., Xu, C., Chen, L., & Zhang, W. (2018) · Metabolic Engineering · doi:10.1016/j.ymben.2018.06.002 · Cited by 112 · see citing works

Light-regulated expression of the psbD gene family in Synecbococcus sp. strain PCC 7942: evidence for the role of duplicated psbD genes in cyanobacteria

Bustos, S. A., & Golden, S. S. (1992) · Molecular and General Genetics MGG · doi:10.1007/bf00280000 · Cited by 111 · see citing works

The sRNA NsiR4 is involved in nitrogen assimilation control in cyanobacteria by targeting glutamine synthetase inactivating factor IF7

Klähn, S., Schaal, C. A. M. V. D., Georg, J., Baumgartner, D., Knippen, G., Hagemann, M., Muro-Pastor, A. M., & Hess, W. R. (2015) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1508412112 · Cited by 111 · see citing works

The circadian clock and darkness control natural competence in cyanobacteria

Taton, A., Erikson, C., Yang, Y., Rubin, B. E., Rifkin, S. A., Golden, J. W., & Golden, S. S. (2020) · Nature Communications · doi:10.1038/s41467-020-15384-9 · Cited by 110 · see citing works

Insertion sequence IS10 anti-sense pairing initiates by an interaction between the 5′ end of the target RNA and a loop in the anti-sense RNA

Kittle, J. D., Simons, R. W., Lee, J. S., & Kleckner, N. E. (1989) · Journal of Molecular Biology · doi:10.1016/0022-2836(89)90132-0 · Cited by 107 · see citing works

A high resolution map of a cyanobacterial transcriptome

Vijayan, V., Jain, I. H., & O’Shea, E. K. (2011) · Genome biology · doi:10.1186/gb-2011-12-5-r47 · Cited by 107 · see citing works

Paired termini stabilize antisense RNAs and enhance conditional gene silencing in Escherichia coli

Nakashima, N., Tamura, T., & Good, L. (2006) · Nucleic Acids Research · doi:10.1093/nar/gkl697 · Cited by 107 · see citing works

MIT Artificial Intelligence Laboratory

Horn, B. K. P., Marr, D., Hollerbach, J. M., et al. (1980) · AI Magazine · doi:10.1609/aimag.v1i1.87 · Cited by 103 · see citing works

SEVA 3.0: an update of the Standard European Vector Architecture for enabling portability of genetic constructs among diverse bacterial hosts

Martínez‐García, E., Goñi‐Moreno, Á., Bartley, B., McLaughlin, J. A., Sánchez-Sampedro, L., del Pozo, H. P., Hernández, C. P., Marletta, A. S., Lucrezia, D. D., Sánchez-Fernández, G., et al. (2019) · Nucleic Acids Research · doi:10.1093/nar/gkz1024 · Cited by 103 · see citing works

Design and analysis of LacI-repressed promoters and DNA-looping in a cyanobacterium

Camsund, D., Heidorn, T., & Lindblad, P. C. (2014) · Journal of Biological Engineering · doi:10.1186/1754-1611-8-4 · Cited by 102 · see citing works

A Genetic Toolbox for Modulating the Expression of Heterologous Genes in the Cyanobacterium Synechocystis sp. PCC 6803

Wang, B., Eckert, C. A., Maness, P., & Yu, J. (2017) · ACS Synthetic Biology · doi:10.1021/acssynbio.7b00297 · Cited by 99 · see citing works

Construction of Shuttle Plasmids Which Can Be Efficiently Mobilized from Escherichia coli into the Chromatically Adapting Cyanobacterium, Fremyella diplosiphon

Cobley, J. G., Zerweck, E. H., Reyes, R., et al. (1993) · Plasmid · doi:10.1006/plas.1993.1037 · Cited by 98 · see citing works

Exploring native genetic elements as plug-in tools for synthetic biology in the cyanobacterium Synechocystis sp. PCC 6803

Liu, D., & Pakrasi, H. B. (2018) · Microbial Cell Factories · doi:10.1186/s12934-018-0897-8 · Cited by 98 · see citing works

Bacteriophage T7 transcription system: an enabling tool in synthetic biology

Wang, W., Li, Y., Wang, Y., et al. (2018) · Biotechnology Advances · doi:10.1016/j.biotechadv.2018.10.001 · Cited by 97 · see citing works

Evidence for the widespread distribution of CRISPR-Cas system in the PhylumCyanobacteria

Cai, F., Axen, S. D., & Kerfeld, C. A. (2013) · RNA Biology · doi:10.4161/rna.24571 · Cited by 96 · see citing works

Expression of the Escherichia coli lacZ Gene on a Plasmid Vector in a Cyanobacterium

Buzby, J. S., Porter, R. D., & Stevens, S. E. (1985) · Science · doi:10.1126/science.2997920 · Cited by 96 · see citing works

Synthetic tolerance: three noncoding small RNAs, DsrA, ArcZ and RprA, acting supra-additively against acid stress

Gaida, S. M., Al-Hinai, M. A., Indurthi, D. C., Nicolaou, S., & Papoutsakis, E. T. (2013) · Nucleic Acids Research · doi:10.1093/nar/gkt651 · Cited by 96 · see citing works

Transformation of the cyanobacterium Synechocystis sp. PCC 6803 as a tool for genetic mapping: optimization of efficiency

Kufryk, G. I., Sachet, M., Schmetterer, G., & Vermaas, W. F. J. (2002) · FEMS Microbiology Letters · doi:10.1111/j.1574-6968.2002.tb11012.x · Cited by 95 · see citing works

A role for the signal transduction protein PII in the control of nitrate/nitrite uptake in a cyanobacterium

Lee, H.-M., Flores, E., Herrero, A., et al. (1998) · FEBS Letters · doi:10.1016/s0014-5793(98)00451-7 · Cited by 93 · see citing works

Transformation of a filamentous cyanobacterium by electroporation

Thiel, T., & Poo, H. (1989) · Journal of Bacteriology · doi:10.1128/jb.171.10.5743-5746.1989 · Cited by 90 · see citing works

Cyanobacteria as Chassis for Industrial Biotechnology: Progress and Prospects

Al‐Haj, L., Lui, Y. T., Abed, R. M. M., Gomaa, M., & Purton, S. (2016) · Life · doi:10.3390/life6040042 · Cited by 90 · see citing works

Photosynthetic conversion of carbon dioxide to ethylene by the recombinant cyanobacterium, Synechococcus sp. PCC 7942, which harbors a gene for the ethylene-forming enzyme of Pseudomonas syringae

Sakai, M., Ogawa, T., Matsuoka, M., & Fukuda, H. (1997) · Journal of Fermentation and Bioengineering · doi:10.1016/s0922-338x(97)82004-1 · Cited by 89 · see citing works

A conjugative plasmid vector for promoter analysis in several cyanobacteria of the genera Synechococcus and Synechocystis

Marraccini, P., Bulteau, S., Cassier‐Chauvat, C., Mermet-Bouvier, P., & Chauvat, F. (1993) · Plant Molecular Biology · doi:10.1007/bf00021546 · Cited by 87 · see citing works

Transformation in Cyanobacteria

Porter, R. D. (1986) · CRC Critical Reviews in Microbiology · doi:10.3109/10408418609108736 · Cited by 83 · see citing works

Fine-Tuning of Photoautotrophic Protein Production by Combining Promoters and Neutral Sites in the Cyanobacterium Synechocystis sp. Strain PCC 6803

Ng, A. H., Berla, B. M., & Pakrasi, H. B. (2015) · Applied and Environmental Microbiology · doi:10.1128/aem.01349-15 · Cited by 83 · see citing works

Translation efficiency of heterologous proteins is significantly affected by the genetic context of RBS sequences in engineered cyanobacterium Synechocystis sp. PCC 6803

Thiel, K., Mulaku, E., Dandapani, H., Nagy, C. I., Aro, E., & Kallio, P. (2018) · Microbial Cell Factories · doi:10.1186/s12934-018-0882-2 · Cited by 83 · see citing works

Gene Transfer to the Desiccation-Tolerant Cyanobacterium Chroococcidiopsis

Billi, D., Friedmann, E. I., Helm, R. F., & Potts, M. (2001) · Journal of Bacteriology · doi:10.1128/jb.183.7.2298-2305.2001 · Cited by 82 · see citing works

Strong and regulated promoters in the cyanobacteriumAnabaenaPCC 7120

Elhai, J. (1993) · FEMS Microbiology Letters · doi:10.1111/j.1574-6968.1993.tb06570.x · Cited by 82 · see citing works

Processes at the nick region link conjugation, T‐DNA transfer and rolling circle replication

Waters, V. L., & Guiney, D. G. (1993) · Molecular Microbiology · doi:10.1111/j.1365-2958.1993.tb01242.x · Cited by 82 · see citing works

Ectopic expression of hetP can partially bypass the need for hetR in heterocyst differentiation by Anabaena sp. strain PCC 7120

Higa, K. C., & Callahan, S. M. (2010) · Molecular Microbiology · doi:10.1111/j.1365-2958.2010.07257.x · Cited by 81 · see citing works

Improved Genetic Transformation of the Thermophilic Cyanobacterium, Thermosynechococcus elongatus BP-1

Iwai, M., Katoh, H., Katayama, M., & Ikeuchi, M. (2004) · Plant and Cell Physiology · doi:10.1093/pcp/pch015 · Cited by 80 · see citing works

A Rhamnose-Inducible System for Precise and Temporal Control of Gene Expression in Cyanobacteria

Kelly, C. L., Taylor, G. M., Hitchcock, A., Torres-Méndez, A., & Heap, J. T. (2018) · ACS Synthetic Biology · doi:10.1021/acssynbio.7b00435 · Cited by 80 · see citing works

Re-direction of carbon flux to key precursor malonyl-CoA via artificial small RNAs in photosynthetic Synechocystis sp. PCC 6803

Sun, T., Li, S., Song, X., Pei, G., Diao, J., Cui, J., Shi, M., Chen, L., & Zhang, W. (2018) · Biotechnology for Biofuels · doi:10.1186/s13068-018-1032-0 · Cited by 80 · see citing works

Construction of new synthetic biology tools for the control of gene expression in the cyanobacterium Synechococcus sp. strain PCC 7002

Zess, E. K., Begemann, M. B., & Pfleger, B. F. (2015) · Biotechnology and Bioengineering · doi:10.1002/bit.25713 · Cited by 79 · see citing works

Predictive combinatorial design of mRNA translation initiation regions for systematic optimization of gene expression levels

Seo, S. W., Yang, J., Cho, H., Yang, J., Kim, S., Park, J. M., Kim, S., & Jung, G. Y. (2014) · Scientific Reports · doi:10.1038/srep04515 · Cited by 78 · see citing works

Engineering of a green‐light inducible gene expression system in S ynechocystis sp. PCC 6803

Abe, K., Miyake, K., Nakamura, M., Kojima, K., Ferri, S. R., Ikebukuro, K., & Sode, K. (2013) · Microbial Biotechnology · doi:10.1111/1751-7915.12098 · Cited by 76 · see citing works

Engineering Isoprene Synthase Expression and Activity in Cyanobacteria

Chaves, J. E., Rueda-Romero, P., Kirst, H., & Melis, A. (2017) · ACS Synthetic Biology · doi:10.1021/acssynbio.7b00214 · Cited by 76 · see citing works

CRISPRi-dCas12a: A dCas12a-Mediated CRISPR Interference for Repression of Multiple Genes and Metabolic Engineering in Cyanobacteria

Choi, S. Y., & Woo, H. M. (2020) · ACS Synthetic Biology · doi:10.1021/acssynbio.0c00091 · Cited by 76 · see citing works

Phosphorylation of the signal transducer PII protein and an additional effector are required for the PII‐mediated regulation of nitrate and nitrite uptake in the cyanobacterium Synechococcus sp. PCC 7942

Lee, H., Flores, E., Forchhammer, K., et al. (2000) · European Journal of Biochemistry · doi:10.1046/j.1432-1327.2000.01043.x · Cited by 75 · see citing works

An AbrB-Like Protein Regulates the Expression of the Bidirectional Hydrogenase in Synechocystis sp. Strain PCC 6803

Oliveira, P., & Lindblad, P. C. (2007) · Journal of Bacteriology · doi:10.1128/jb.01605-07 · Cited by 75 · see citing works

A glutamine riboswitch is a key element for the regulation of glutamine synthetase in cyanobacteria

Klähn, S., Bolay, P., Wright, P. R., Atilho, R. M., Brewer, K. I., Hagemann, M., Breaker, R. R., & Hess, W. R. (2018) · Nucleic Acids Research · doi:10.1093/nar/gky709 · Cited by 75 · see citing works

SEVA 3.0: an update of the Standard European Vector Architecture for enabling portability of genetic constructs among diverse bacterial hosts

Martínez‐García, E., Goñi‐Moreno, Á., Bartley, B., McLaughlin, J. A., Sánchez-Sampedro, L., del Pozo, H. P., Hernández, C. P., Marletta, A. S., Lucrezia, D. D., Sánchez-Fernández, G., et al. (2020) · Nucleic Acids Research · doi:10.1093/nar/gkaa114 · Cited by 75 · see citing works

An Organic Acid Based Counter Selection System for Cyanobacteria

Begemann, M. B., Zess, E. K., Walters, E. M., Schmitt, E. F., Markley, A. L., & Pfleger, B. F. (2013) · PLoS ONE · doi:10.1371/journal.pone.0076594 · Cited by 72 · see citing works

Construction of bioluminescent cyanobacterial reporter strains for detection of nickel, cobalt and zinc

Peca, L., Kós, P. B., Máté, Z., Farsang, A., & Vass, I. (2008) · FEMS Microbiology Letters · doi:10.1111/j.1574-6968.2008.01393.x · Cited by 72 · see citing works

Evolution of the ssrA degradation tag in Mycoplasma : Specificity switch to a different protease

Gur, E., & Sauer, R. T. (2008) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0808802105 · Cited by 71 · see citing works

Amplified expression of a transcriptional pattern formed during development of Anabaena

Wölk, C. P., Elhai, J., Kuritz, T., & Holland, D. (1993) · Molecular Microbiology · doi:10.1111/j.1365-2958.1993.tb01135.x · Cited by 71 · see citing works

Construction and analysis of an artificial consortium based on the fast-growing cyanobacterium Synechococcus elongatus UTEX 2973 to produce the platform chemical 3-hydroxypropionic acid from CO2

Zhang, L., Chen, L., Diao, J., Song, X., Shi, M., & Zhang, W. (2020) · Biotechnology for Biofuels · doi:10.1186/s13068-020-01720-0 · Cited by 71 · see citing works

Development of SyneBrick Vectors As a Synthetic Biology Platform for Gene Expression in Synechococcus elongatus PCC 7942

Kim, W. J., Lee, S.-M., Um, Y., Sim, S. J., & Woo, H. M. (2017) · Frontiers in Plant Science · doi:10.3389/fpls.2017.00293 · Cited by 70 · see citing works

Gene Delivery Technologies with Applications in Microalgal Genetic Engineering

Gutiérrez, S., & Lauersen, K. J. (2021) · Biology · doi:10.3390/biology10040265 · Cited by 68 · see citing works

Comparative Dose–Response Analysis of Inducible Promoters in Cyanobacteria

Behle, A., Saake, P., Germann, A. T., Dienst, D., & Axmann, I. M. (2020) · ACS Synthetic Biology · doi:10.1021/acssynbio.9b00505 · Cited by 67 · see citing works

Application of CRISPR Interference for Metabolic Engineering of the Heterocyst-Forming Multicellular Cyanobacterium Anabaena sp. PCC 7120

Higo, A., Isu, A., Fukaya, Y., Ehira, S., & Hisabori, T. (2017) · Plant and Cell Physiology · doi:10.1093/pcp/pcx166 · Cited by 65 · see citing works

Improving aSynechocystis-based photoautotrophic chassis through systematic genome mapping and validation of neutral sites

Pinto, F., Pacheco, C. C., Oliveira, P., Montagud, A., Landels, A. R., Couto, N. A. S., Wright, P. C., Urchueguía, J. F., & Tamagnini, P. (2015) · DNA Research · doi:10.1093/dnares/dsv024 · Cited by 63 · see citing works

Biocatalysis in Green and Blue: Cyanobacteria

Jodlbauer, J., Rohr, T., Oliver Spadiut, A., Mihovilovič, M. D., & Rudroff, F. (2021) · Trends in biotechnology · doi:10.1016/j.tibtech.2020.12.009 · Cited by 63 · see citing works

Self-replicating shuttle vectors based on pANS, a small endogenous plasmid of the unicellular cyanobacterium Synechococcus elongatus PCC 7942

Chen, Y., Taton, A., Go, M. C., London, R. E., Pieper, L. M., Golden, S. S., & Golden, J. W. (2016) · Microbiology · doi:10.1099/mic.0.000377 · Cited by 62 · see citing works

Thermosynechococcus as a thermophilic photosynthetic microbial cell factory for CO2 utilisation

Liang, Y., Tang, J., Luo, Y., Kaczmarek, M. B., Li, X., & Daroch, M. (2019) · Bioresource Technology · doi:10.1016/j.biortech.2019.01.089 · Cited by 62 · see citing works

Increased ethylene production by overexpressing phosphoenolpyruvate carboxylase in the cyanobacterium Synechocystis PCC 6803

Durall, C., Lindberg, P., Yu, J., & Lindblad, P. C. (2020) · Biotechnology for Biofuels · doi:10.1186/s13068-020-1653-y · Cited by 62 · see citing works

Emerging Species and Genome Editing Tools: Future Prospects in Cyanobacterial Synthetic Biology

Gale, G. A. R., Schiavon Osorio, A. A., Mills, L. A., Wang, B., Lea-Smith, D. J., & McCormick, A. J. (2019) · Microorganisms · doi:10.3390/microorganisms7100409 · Cited by 61 · see citing works

A hybrid plasmid is a stable cloning vector for the cyanobacterium Anacystis nidulans R2

Golden, S. S., & Sherman, L. A. (1983) · Journal of Bacteriology · doi:10.1128/jb.155.3.966-972.1983 · Cited by 60 · see citing works

A novel counter‐selection method for markerless genetic modification in Synechocystis sp. PCC 6803

Cheah, Y. E., Albers, S. C., & Peebles, C. A. M. (2012) · Biotechnology Progress · doi:10.1002/btpr.1661 · Cited by 59 · see citing works

Heterocyst-Specific Expression of patB , a Gene Required for Nitrogen Fixation in Anabaena sp. Strain PCC 7120

Jones, K. M., Buikema, W. J., & Haselkorn, R. (2003) · Journal of Bacteriology · doi:10.1128/jb.185.7.2306-2314.2003 · Cited by 59 · see citing works

Switching of metabolic programs in response to light availability is an essential function of the cyanobacterial circadian output pathway

Puszynska, A. M., & O’Shea, E. K. (2017) · eLife · doi:10.7554/elife.23210 · Cited by 59 · see citing works

Expanding the toolbox for Synechocystis sp. PCC 6803: validation of replicative vectors and characterization of a novel set of promoters

Ferreira, E. A., Pacheco, C. C., Pinto, F., Pereira, J. A., Lamosa, P., Oliveira, P., Kirov, B., Jaramillo, A., & Tamagnini, P. (2018) · Synthetic Biology · doi:10.1093/synbio/ysy014 · Cited by 59 · see citing works

Tunable Repression of Key Photosynthetic Processes Using Cas12a CRISPR Interference in the Fast-Growing Cyanobacterium Synechococcus sp. UTEX 2973

Knoot, C. J., Biswas, S., & Pakrasi, H. B. (2019) · ACS Synthetic Biology · doi:10.1021/acssynbio.9b00417 · Cited by 59 · see citing works

Synthetic Biology Toolkits for Metabolic Engineering of Cyanobacteria

Xia, P., Ling, H., Foo, J. L., & Chang, M. W. (2019) · Biotechnology Journal · doi:10.1002/biot.201800496 · Cited by 58 · see citing works

Natural transformation of the thermophilic cyanobacterium Thermosynechococcus elongatus BP-1: a simple and efficient method for gene transfer

Onai, K., Morishita, M., Kaneko, T., Tabata, S., & Ishiura, M. (2003) · Molecular Genetics and Genomics · doi:10.1007/s00438-003-0953-9 · Cited by 57 · see citing works

Biotechnology of cyanobacterial isoprene production

Chaves, J. E., & Melis, A. (2018) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-018-9093-3 · Cited by 57 · see citing works

Conjugative transfer and autonomous replication of a promiscuous IncQ plasmid in the cyanobacterium Synechocystis PCC 6803

Kreps, S., Ferino, F., Mosrin, C., et al. (1990) · Molecular and General Genetics MGG · doi:10.1007/bf00280378 · Cited by 56 · see citing works

BioBrick Assembly Standards and Techniques and Associated Software Tools

Røkke, G. B., Korvald, E., Pahr, J., et al. (2013) · Methods in molecular biology · doi:10.1007/978-1-62703-764-8_1 · Cited by 56 · see citing works

Functional Elements of the Strong psbAI Promoter of Synechococcus elongatus PCC 7942

Nair, U., Thomas, C., & Golden, S. S. (2001) · Journal of Bacteriology · doi:10.1128/jb.183.5.1740-1747.2001 · Cited by 55 · see citing works

Modification of RSF1010-Based Broad-Host-Range Plasmids for Improved Conjugation and Cyanobacterial Bioprospecting

Bishé, B., Taton, A., & Golden, J. W. (2019) · iScience · doi:10.1016/j.isci.2019.09.002 · Cited by 55 · see citing works

Oxygen‐responsive genetic circuits constructed in Synechocystis sp. PCC 6803

Immethun, C. M., Ng, K. M., DeLorenzo, D. M., Waldron‐Feinstein, B., Lee, Y.-C. J., & Moon, T. S. (2015) · Biotechnology and Bioengineering · doi:10.1002/bit.25722 · Cited by 54 · see citing works

Regulatory systems for gene expression control in cyanobacteria

Till, P., Toepel, J., Bühler, B., Mach, R. L., & Mach-Aigner, A. R. (2020) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-019-10344-w · Cited by 54 · see citing works

Cyanobacterial defense mechanisms against foreign DNA transfer and their impact on genetic engineering

Stucken, K., Koch, R., & Dagan, T. (2013) · Biological Research · doi:10.4067/s0716-97602013000400009 · Cited by 52 · see citing works

Repression by Fur is not the main mechanism controlling the iron-inducibleisiABoperon in the cyanobacteriumSynechocystissp. PCC 6803

Kunert, A., Vinnemeier, J., Erdmann, N., & Hagemann, M. (2003) · FEMS Microbiology Letters · doi:10.1016/s0378-1097(03)00689-x · Cited by 52 · see citing works

Photosynthetic Co-production of Succinate and Ethylene in a Fast-Growing Cyanobacterium, Synechococcus elongatus PCC 11801

Sengupta, A., Pritam, P., Jaiswal, D., Bandyopadhyay, A., Pakrasi, H. B., & Wangikar, P. P. (2020) · Metabolites · doi:10.3390/metabo10060250 · Cited by 52 · see citing works

Characterization of Stress Responses of Heavy Metal and Metalloid Inducible Promoters in Synechocystis PCC6803

Blasi, B. (2012) · Journal of Microbiology and Biotechnology · doi:10.4014/jmb.1106.06050 · Cited by 51 · see citing works

Chloroplast promoter driven expression of the chloramphenicol acetyl transferase gene in a cyanobacterium

Dzelzkalns, V. A., Owens, G. C., & Bogorad, L. (1984) · Nucleic Acids Research · doi:10.1093/nar/12.23.8917 · Cited by 50 · see citing works

The Flavodiiron Protein Flv3 Functions as a Homo-Oligomer During Stress Acclimation and is Distinct from the Flv1/Flv3 Hetero-Oligomer Specific to the O2Photoreduction Pathway

Mustila, H., Paananen, P., Battchikova, N., Santana‐Sánchez, A., Muth‐Pawlak, D., Hagemann, M., Aro, E., & Allahverdiyeva, Y. (2016) · Plant and Cell Physiology · doi:10.1093/pcp/pcw047 · Cited by 50 · see citing works

Cyanobacterial sigma factors: Current and future applications for biotechnological advances

Srivastava, A., Summers, M. L., & Sobotka, R. (2020) · Biotechnology Advances · doi:10.1016/j.biotechadv.2020.107517 · Cited by 49 · see citing works

Recent advances in synthetic biology of cyanobacteria for improved chemicals production

Wang, F., Gao, Y., & Yang, G. (2020) · Bioengineered · doi:10.1080/21655979.2020.1837458 · Cited by 49 · see citing works

Stability of the Synechococcus elongatus PCC 7942 circadian clock under directed anti-phase expression of the kai genes

Ditty, J. L., Canales, S. R., Anderson, B. E., Williams, S. B., & Golden, S. S. (2005) · Microbiology · doi:10.1099/mic.0.28030-0 · Cited by 48 · see citing works

A tightly inducible riboswitch system in Synechocystis sp. PCC 6803

Ohbayashi, R., Akai, H., Yoshikawa, H., Hess, W. R., & Watanabe, S. (2016) · The Journal of General and Applied Microbiology · doi:10.2323/jgam.2016.02.002 · Cited by 48 · see citing works

Paired cloning vectors for complementation of mutations in the cyanobacterium Anabaena sp. strain PCC 7120

Wölk, C. P., Fan, Q., Zhou, R., Huang, G., Lechno‐Yossef, S., Kuritz, T., & Wojciuch, E. (2007) · Archives of Microbiology · doi:10.1007/s00203-007-0276-z · Cited by 47 · see citing works

Transformation and Conjugal Transfer of Foreign Genes into the Filamentous Multicellular Cyanobacteria (Subsection V) Fischerella and Chlorogloeopsis

Stucken, K., Ilhan, J., Roettger, M., Dagan, T., & Martin, W. F. (2012) · Current Microbiology · doi:10.1007/s00284-012-0193-5 · Cited by 47 · see citing works

Genetic, Genomics, and Responses to Stresses in Cyanobacteria: Biotechnological Implications

Cassier‐Chauvat, C., Blanc-Garin, V., & Chauvat, F. (2021) · Genes · doi:10.3390/genes12040500 · Cited by 47 · see citing works

Construction of a Shuttle Vector Using an Endogenous Plasmid From the Cyanobacterium Synechocystis sp. PCC6803

Jin, H., Wang, Y., Idoine, A., & Bhaya, D. (2018) · Frontiers in Microbiology · doi:10.3389/fmicb.2018.01662 · Cited by 46 · see citing works

Improving heterologous protein expression in Synechocystis sp. PCC 6803 for alpha-bisabolene production

Sebesta, J., & Peebles, C. A. (2020) · Metabolic Engineering Communications · doi:10.1016/j.mec.2019.e00117 · Cited by 46 · see citing works

Construction of a hybrid plasmid capable of replication in the bacterium Escherichia coli and the cyanobacterium Anacystis nidulans

Sherman, L. A., & van de Putte, P. (1982) · Journal of Bacteriology · doi:10.1128/jb.150.1.410-413.1982 · Cited by 45 · see citing works

Construction and use of GFP reporter vectors for analysis of cell-type-specific gene expression in Nostoc punctiforme

Argueta, C., Yuksek, K., & Summers, M. L. (2004) · Journal of Microbiological Methods · doi:10.1016/j.mimet.2004.06.009 · Cited by 45 · see citing works

mRNA stability is regulated by a coding‐region element and the unique 5′ untranslated leader sequences of the three Synechococcus psbA transcripts

Kulkarni, R. D., & Golden, S. S. (1997) · Molecular Microbiology · doi:10.1046/j.1365-2958.1997.4201768.x · Cited by 45 · see citing works

Genomics Approaches to Deciphering Natural Transformation in Cyanobacteria

Wendt, K. E., & Pakrasi, H. B. (2019) · Frontiers in Microbiology · doi:10.3389/fmicb.2019.01259 · Cited by 45 · see citing works

A single vector-based strategy for marker-less gene replacement in Synechocystis sp. PCC 6803

Viola, S., Rühle, T., & Leister, D. (2014) · Microbial Cell Factories · doi:10.1186/1475-2859-13-4 · Cited by 44 · see citing works

Metabolic engineering of Synechocystis sp. PCC 6803 for the photoproduction of the sesquiterpene valencene

Dietsch, M., Behle, A., Westhoff, P., & Axmann, I. M. I. (2021) · Metabolic Engineering Communications · doi:10.1016/j.mec.2021.e00178 · Cited by 44 · see citing works

Synechocystis sp. PCC 6803 overexpressing genes involved in CBB cycle and free fatty acid cycling enhances the significant levels of intracellular lipids and secreted free fatty acids

Eungrasamee, K., Incharoensakdi, A., Lindblad, P. C., & Jantaro, S. (2020) · Scientific Reports · doi:10.1038/s41598-020-61100-4 · Cited by 43 · see citing works

Current understanding of the cyanobacterial CRISPR-Cas systems and development of the synthetic CRISPR-Cas systems for cyanobacteria

Pattharaprachayakul, N., Lee, M., Incharoensakdi, A., & Woo, H. M. (2020) · Enzyme and Microbial Technology · doi:10.1016/j.enzmictec.2020.109619 · Cited by 43 · see citing works

Structure and regulation of genes encoding phycocyanin and allophycocyanin from Anabaena variabilis ATCC 29413

Johnson, T. R., Haynes, J. I., Wealand, J. L., et al. (1988) · Journal of Bacteriology · doi:10.1128/jb.170.4.1858-1865.1988 · Cited by 42 · see citing works

Characterization of the activity of heavy metal-responsive promoters in the cyanobacteriumSynechocystisPCC 6803

Peca, L., Kós, P. B., & Vass, I. (2007) · Acta Biologica Hungarica · doi:10.1556/abiol.58.2007.suppl.2 · Cited by 41 · see citing works

Cyanobacterial multi-copy chromosomes and their replication

Watanabe, S. (2020) · Bioscience Biotechnology and Biochemistry · doi:10.1080/09168451.2020.1736983 · Cited by 41 · see citing works

Cyanobacteria as whole-cell factories: Current status and future prospectives

Toepel, J., Karande, R., Klähn, S., & Bühler, B. (2023) · Current Opinion in Biotechnology · doi:10.1016/j.copbio.2023.102892 · Cited by 52 · see citing works

Customized molecular tools to strengthen metabolic engineering of cyanobacteria

Klähn, S., Opel, F., & Hess, W. R. (2024) · Green Carbon · doi:10.1016/j.greenca.2024.05.002 · Cited by 27 · see citing works

Co-expression of auxiliary genes enhances the activity of a heterologous O2-tolerant hydrogenase in the cyanobacterium Synechocystis sp. PCC 6803

Lupacchini, S., Stauder, R., Opel, F., Klähn, S., Schmid, A., Bühler, B., & Toepel, J. (2025) · Biotechnology for Biofuels and Bioproducts · doi:10.1186/s13068-025-02634-5 · Cited by 12 · see citing works

Cyanobacteria as a Photosynthetic Chassis for Metabolic Pathway Engineering with Heterologous Gene Expression

Walshe, J., & Saha, S. K. (2026) · Current Issues in Molecular Biology · doi:10.3390/cimb48060638 · Cited by 1 · see citing works

Susquehanna Chorale Spring Concert "Roots and Wings"

Hawkins, D. (2017)

Using DataGrid Control to Realize DataBase of Querying in VB6.0

Hu, S. (2000) · Modern Computer

Study and Two Types of Typical Usage of DataGrid Web Server Control

Hai-tao, L. (2005) · Modern Computer

Achieving Parameter of DBSCAN Based on Datagrid

Xu, Q. (2010)

PACWON: A parallelizing compiler for workstations on a network

Das, D. K., & Das, P. P. (1998) · Journal of Systems Architecture · doi:10.1016/s1383-7621(97)00078-7