The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Cloning and expression of the Mycobacterium bovis BCG gene for extracellular alpha antigen
Open accessAbstract
The gene for the extracellular alpha antigen of Mycobacterium bovis BCG was cloned by using a single probe restricted to G or C in the third position. This technique should have great potential for the isolation of mycobacterial antigen genes. The gene analysis revealed that the alpha antigen gene encoded 323 amino acid residues, including 40 amino acids for signal peptide followed by 283 amino acids for mature protein. This is the first report on the structure of the mycobacterial signal peptide. The promoter-like sequence and ribosome-binding site were observed upstream of the open reading frame. In the coding region, the third position of the codon showed high G + C content (86%). The gene was expressed as an unfused protein in Escherichia coli by using an E. coli expression vector. This protein, which reacted with polyclonal antibody raised against alpha antigen from Mycobacterium tuberculosis, would be applicable to the immunodiagnosis of tuberculosis.
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Citations
Cites
Introduction of foreign DNA into mycobacteria using a shuttle phasmid
Jacobs, W. R., Tuckman, M., & Bloom, B. R. (1987) · Nature · doi:10.1038/327532a0 · Cited by 314 · cited 1×
“bovis BCG with mycobacteriophage has recently been reported (15).”
Background
Cited by
MSCRAMM-MEDIATED ADHERENCE OF MICROORGANISMS TO HOST TISSUES
Patti, J. M., Allen, B. L., McGavin, M. J., & Höök, M. (1994) · Annual Review of Microbiology · doi:10.1146/annurev.mi.48.100194.003101 · Cited by 1,191
Sørensen, A. L., Nagai, S., Houen, G., Andersen, P., & Andersen, A. B. (1995) · Infection and Immunity · doi:10.1128/iai.63.5.1710-1717.1995 · Cited by 586
Harboe, M., Oettinger, T., Wiker, H. G., Rosenkrands, I., & Andersen, P. (1996) · Infection and Immunity · doi:10.1128/iai.64.1.16-22.1996 · Cited by 465
Bacterial proteins binding to the mammalian extracellular matrix
Westerlund, B., & Korhonen, T. K. (1993) · Molecular Microbiology · doi:10.1111/j.1365-2958.1993.tb01729.x · Cited by 313
Role of fibronectin-binding MSCRAMMs in bacterial adherence and entry into mammalian cells
Joh, D., Wann, E. R., Kreikemeyer, B., Speziale, P., & Höök, M. (1999) · Matrix Biology · doi:10.1016/s0945-053x(99)00025-6 · Cited by 278
Metadata
- Authors
- Matsuo, K., Yamaguchi, R., Yamazaki, A., Tasaka, H., & Yamada, T.
- Venue
- Journal of Bacteriology
- Publisher
- American Society for Microbiology
- Published
- 1988-09
- Volume
- 170
- Issue
- 9
- Pages
- 3847-3854
- ISSN
- 0021-9193 (ISSN-L)
- 1098-5530
- OpenAlex
- W2154213475
- Cited by
- 168
- References
- 1
- FWCI
- 12.62
- Type
- journal article
- Language
- en
- Open access
- green
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- Matsuo, K., Yamaguchi, R., Yamazaki, A., Tasaka, H., & Yamada, T. (1988). Cloning and expression of the Mycobacterium bovis BCG gene for extracellular alpha antigen. Journal of Bacteriology. https://doi.org/10.1128/jb.170.9.3847-3854.1988
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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