The DOI matches its Crossref record; 29 of the 29 references it cites were found in a registry or an index.
Evidence for occurrence of the ESAT-6 protein in Mycobacterium tuberculosis and virulent Mycobacterium bovis and for its absence in Mycobacterium bovis BCG
Open accessAbstract
ESAT-6 is a secreted protein present in the short-term culture filtrate of Mycobacterium tuberculosis after growth on a synthetic Sauton medium. ESAT-6 has recently been demonstrated to induce strong T-cell responses in a mouse model of memory immunity after infection with M. tuberculosis. In Western blotting (immunoblotting), the monoclonal antibody HYB76-8, reacting with ESAT-6, gave a 6-kDa region was observed in filtrates from four of eight substrains of M. bovis BCG that produced high levels of MPB64, while no band occurred in the 6-kDa region with any of these BCG substrains. Southern blotting and PCR experiments with genomic mycobacterial DNA showed the presence of the esat-6 gene in reference strains and clinical isolates of M. tuberculosis as well as in virulent M. bovis. The esat-6 gene could not be demonstrated in any of the eight substrains of M. bovis BCG tested by these techniques. Two gene deletions that distinguish M. bovis BCG from virulent M. bovis have thus now been demonstrated. Deletion of mpb64 affects four of the eight substrains tested; deletion of esat-6 affects all of them. The reaction of HYB76-8 AT 26 kDa with four of the BCG substrains was demonstrated to result from cross-reactivity with MPB64. HYB76-8 was also shown to cross-react with the A, B, and C components of the antigen 85 complex and MPT51.
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Cites
Sørensen, A. L., Nagai, S., Houen, G., Andersen, P., & Andersen, A. B. (1995) · Infection and Immunity · doi:10.1128/iai.63.5.1710-1717.1995 · Cited by 586
Self-citationThe antigen 85 complex: a major secretion product of Mycobacterium tuberculosis
Wiker, H. G., & Harboe, M. (1992) · Microbiological Reviews · doi:10.1128/mmbr.56.4.648-661.1992 · Cited by 491
Self-citationAndersen, P. (1994) · Infection and Immunity · doi:10.1128/iai.62.6.2536-2544.1994 · Cited by 416
Self-citationRecall of long-lived immunity to Mycobacterium tuberculosis infection in mice.
Andersen, P., Andersen, A. B., Sørensen, A. L., & Nagai, S. (1995) · The Journal of Immunology · doi:10.4049/jimmunol.154.7.3359 · Cited by 351
Self-citationNagai, S., Wiker, H. G., Harboe, M., & Kinomoto, M. (1991) · Infection and Immunity · doi:10.1128/iai.59.1.372-382.1991 · Cited by 319
Self-citationProteins released from Mycobacterium tuberculosis during growth
Andersen, P., Askgaard, D., Ljungqvist, L., Bennedsen, J., & Heron, I. (1991) · Infection and Immunity · doi:10.1128/iai.59.6.1905-1910.1991 · Cited by 305
Self-citationProperties of proteins MPB64, MPB70, and MPB80 of Mycobacterium bovis BCG
Harboe, M., Nagai, S., Patarroyo, M. E., Torres, M. L., Ramirez, C., & Cruz, N. (1986) · Infection and Immunity · doi:10.1128/iai.52.1.293-302.1986 · Cited by 207
Self-citationAndersen, A. B., & Hansen, E. B. (1989) · Infection and Immunity · doi:10.1128/iai.57.8.2481-2488.1989 · Cited by 183
Cloning and expression of the Mycobacterium bovis BCG gene for extracellular alpha antigen
Matsuo, K., Yamaguchi, R., Yamazaki, A., Tasaka, H., & Yamada, T. (1988) · Journal of Bacteriology · doi:10.1128/jb.170.9.3847-3854.1988 · Cited by 168
Specific skin-reactive protein from culture filtrate of Mycobacterium bovis BCG
Nagai, S., Matsumoto, J., & Nagasuga, T. (1981) · Infection and Immunity · doi:10.1128/iai.31.3.1152-1160.1981 · Cited by 129
Cited by
Lewinsohn, D. M., Leonard, M. K., LoBue, P. A., Cohn, D. L., Daley, C. L., Desmond, E., Keane, J., Lewinsohn, D. A., Loeffler, A. M., Mazurek, G. H., et al. (2017) · Clinical Infectious Diseases · doi:10.1093/cid/ciw694 · Cited by 992
“bovis (BCG strains) and most nontuberculous mycobacteria with the important exceptions of Mycobacterium kansasii, Mycobacterium szulgai, Mycobacterium marinum [32, 38], and Mycobacterium leprae [39, 40].”
BackgroundM. tuberculosis and M. leprae Translocate from the Phagolysosome to the Cytosol in Myeloid Cells
van der Wel, N., Hava, D., Houben, D., Fluitsma, D., van Zon, M., Pierson, J., Brenner, M., & Peters, P. J. (2007) · Cell · doi:10.1016/j.cell.2007.05.059 · Cited by 960
Specific immune-based diagnosis of tuberculosis
Andersen, P., Munk, M., Pollock, J., & Doherty, T. (2000) · The Lancet · doi:10.1016/s0140-6736(00)02742-2 · Cited by 924
Self-citationA systematic review of rapid diagnostic tests for the detection of tuberculosis infection
Dinnes, J., Deeks, J., Kunst, H., Gibson, A., Cummins, E., Waugh, N., Drobniewski, F., & Lalvani, A. (2007) · Health Technology Assessment · doi:10.3310/hta11030 · Cited by 666
Recombinant BCG exporting ESAT-6 confers enhanced protection against tuberculosis
Pym, A. S., Brodin, P., Majlessi, L., Brosch, R., Demangel, C., Williams, A., Griffiths, K. E., Marchal, G., Leclerc, C., & Cole, S. T. (2003) · Nature Medicine · doi:10.1038/nm859 · Cited by 644
Metadata
- Authors
- Harboe, M., Oettinger, T., Wiker, H. G., Rosenkrands, I., & Andersen, P.
- Venue
- Infection and Immunity
- Publisher
- American Society for Microbiology
- Published
- 1996-01
- Volume
- 64
- Issue
- 1
- Pages
- 16-22
- ISSN
- 0019-9567 (ISSN-L)
- 1098-5522
- OpenAlex
- W1874659400
- Cited by
- 465
- References
- 29
- FWCI
- 13.52
- Type
- journal article
- Language
- en
- Open access
- green
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- CoChat. (2026). Citation check: Evidence for occurrence of the ESAT-6 protein in Mycobacterium tuberculosis and virulent Mycobacterium bovis and for its absence in Mycobacterium bovis BCG. https://app.cochat.ai/papers/10.1128/iai.64.1.16-22.1996
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- Harboe, M., Oettinger, T., Wiker, H. G., Rosenkrands, I., & Andersen, P. (1996). Evidence for occurrence of the ESAT-6 protein in Mycobacterium tuberculosis and virulent Mycobacterium bovis and for its absence in Mycobacterium bovis BCG. Infection and Immunity. https://doi.org/10.1128/iai.64.1.16-22.1996
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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